FastQCFastQC Report
Fri 10 Feb 2017
ERR1631631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19818
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA490.24724997477041075No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC440.22202038550812395No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA440.22202038550812395No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA390.19679079624583712No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA350.17660712483600768No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT350.17660712483600768No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA350.17660712483600768No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA320.1614693712786356No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA320.1614693712786356No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA300.15137753557372086No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG290.14633161772126352No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA270.13623978201634876No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC260.13119386416389142No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC250.12614794631143406No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA250.12614794631143406No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC240.12110202845897669No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT240.12110202845897669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230.11605611060651934No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC230.11605611060651934No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA230.11605611060651934No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA230.11605611060651934No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT210.1059642749016046No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG210.1059642749016046No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT200.10091835704914726No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT200.10091835704914726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA508.51679E-625.90000237
GGTATCA400.00186533123.1251
ATCAACG1002.538236E-718.52
TCAACGC1054.2381544E-717.6190473
TATCAAC1054.2381544E-717.6190471
CAGAGTA1106.9020643E-716.8181829
AGAGTAC1106.9020643E-716.81818210
GCAGAGT1106.9020643E-716.8181828
CGCAGAG1106.9020643E-716.8181827
CAACGCA1106.9020643E-716.8181824
CTCTTAT803.1775446E-416.187536
ACGCAGA1151.0987551E-616.0869566
AACGCAG1252.620418E-614.7999995
AGTACGG907.779485E-414.38888912
GAGTACG907.779485E-414.38888911
TCTCTTA1303.9382794E-614.23076937
GTACGGG950.001171178713.63157913
GTCTCTT1654.5843273E-511.21212136
CCTGTCT1655.8292446E-410.09090937
TGTCTCT2156.631839E-48.60465135
Produced by FastQC (version 0.11.5)