##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631631.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19818
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57180341104047	34.0	31.0	34.0	31.0	34.0
2	32.744171964880415	34.0	31.0	34.0	31.0	34.0
3	32.81400746795842	34.0	31.0	34.0	31.0	34.0
4	36.26450701382581	37.0	37.0	37.0	35.0	37.0
5	36.197345847209604	37.0	35.0	37.0	35.0	37.0
6	36.25315369865778	37.0	37.0	37.0	35.0	37.0
7	36.23932788374205	37.0	37.0	37.0	35.0	37.0
8	36.22615803814714	37.0	36.0	37.0	35.0	37.0
9	38.06620244222424	39.0	38.0	39.0	35.0	39.0
10	37.95443536179231	39.0	38.0	39.0	35.0	39.0
11	38.02457361994147	39.0	38.0	39.0	35.0	39.0
12	37.96997678877788	39.0	38.0	39.0	35.0	39.0
13	38.02048642648098	39.0	38.0	39.0	35.0	39.0
14	39.37365021697447	40.0	39.0	41.0	37.0	41.0
15	39.39822383691593	40.0	39.0	41.0	37.0	41.0
16	39.361792310021194	40.0	39.0	41.0	36.0	41.0
17	39.31658088606317	40.0	39.0	41.0	36.0	41.0
18	39.32561307901907	40.0	39.0	41.0	36.0	41.0
19	39.39696235745282	40.0	39.0	41.0	37.0	41.0
20	39.38121909375315	40.0	39.0	41.0	36.0	41.0
21	39.36370975880513	40.0	39.0	41.0	36.0	41.0
22	39.315268947421536	40.0	39.0	41.0	36.0	41.0
23	39.26168129982844	40.0	39.0	41.0	36.0	41.0
24	39.252951861943686	40.0	39.0	41.0	36.0	41.0
25	39.209758805126654	40.0	39.0	41.0	36.0	41.0
26	39.123271773135535	40.0	39.0	41.0	36.0	41.0
27	39.025885558583106	40.0	39.0	41.0	35.0	41.0
28	39.00736704006459	40.0	39.0	41.0	35.0	41.0
29	39.00938540720557	40.0	39.0	41.0	35.0	41.0
30	38.97729336966394	40.0	38.0	41.0	35.0	41.0
31	38.845695832071854	40.0	38.0	41.0	35.0	41.0
32	38.81219093753154	40.0	38.0	41.0	35.0	41.0
33	38.77278231910385	40.0	38.0	41.0	35.0	41.0
34	38.783883338379255	40.0	38.0	41.0	35.0	41.0
35	38.71308911090927	40.0	38.0	41.0	35.0	41.0
36	38.67993743061863	40.0	38.0	41.0	35.0	41.0
37	38.606721162579476	40.0	38.0	41.0	35.0	41.0
38	38.57760621657079	40.0	38.0	41.0	35.0	41.0
39	38.52416994651327	40.0	38.0	41.0	34.0	41.0
40	38.22873145625189	40.0	38.0	41.0	34.0	41.0
41	38.293218286406294	40.0	38.0	41.0	34.0	41.0
42	38.29054394994449	40.0	37.0	41.0	34.0	41.0
43	37.84862246442628	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	6.0
23	2.0
24	11.0
25	16.0
26	33.0
27	43.0
28	70.0
29	90.0
30	141.0
31	166.0
32	267.0
33	335.0
34	506.0
35	755.0
36	1140.0
37	2106.0
38	4812.0
39	9318.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.8457967504289	17.024926834191138	13.321223130487436	27.80805328489252
2	20.703400948632556	19.820365324452517	32.9347058229892	26.541527903925726
3	20.269452013321224	19.810273488747605	31.002119285498033	28.918155212433145
4	15.415279039257241	16.348773841961854	34.51912402866081	33.71682309012009
5	16.50519729538803	33.61590473307095	34.18609345039863	15.692804521142396
6	33.802603693611864	33.787465940054496	16.01069734584721	16.399233020486427
7	29.634675547482086	28.428701180744774	20.44605913815723	21.490564133615905
8	26.793823796548594	32.80351195882531	20.17862549197699	20.224038752649108
9	28.25209405590877	12.912503784438389	17.796952265617115	41.03844989403573
10	19.235038853567463	23.806640427893832	30.361287718235946	26.597033000302755
11	39.00494499949541	19.653850035321422	18.735492986174187	22.605711979008984
12	22.600666061156524	24.49793117368049	26.88465031789282	26.016752447270157
13	35.61913412049652	17.287314562518922	21.107074376829146	25.986476940155413
14	23.958017963467555	20.668079523665355	23.281864971238267	32.09203754162882
15	29.140175597941266	24.911696437581995	20.516701988091633	25.431425976385103
16	24.841053587647593	24.38692098092643	22.963972146533454	27.80805328489252
17	26.415379957614288	25.068119891008173	21.793319204763346	26.72318094661419
18	26.14794631143405	21.853870218992835	23.438288424664446	28.559895044908668
19	28.494298112826723	22.696538500353213	22.847916035926936	25.961247350893128
20	30.2603693611868	21.03643152689474	22.746997678877786	25.956201433040672
21	27.364012513876272	22.479564032697546	22.232314057927137	27.92410939549904
22	28.605308305580785	23.519023110303763	21.016247855484913	26.85942072863054
23	27.75759410636795	22.030477343828842	23.256635381975983	26.955293167827225
24	27.26309415682713	22.09102835805833	23.589665960238168	27.05621152487637
25	27.136946210515696	22.484609950550006	23.413058835402158	26.965385003532145
26	26.83419113936825	23.3020486426481	22.948834392976085	26.91492582500757
27	26.86951256433545	22.161671207992732	24.42224240589363	26.54657382177818
28	24.866283176909878	24.2960944595822	22.651125239681097	28.186497123826825
29	27.01079826420426	23.917650620647894	22.863053789484308	26.208497325663537
30	26.829145221515795	22.923604803713797	23.81673226359875	26.430517711171664
31	26.88465031789282	23.478655767484106	23.463518013926734	26.17317590069634
32	25.13876274094258	23.080028257139972	23.251589464123523	28.529619537793927
33	25.290140276516297	22.83782420022202	24.931880108991823	26.940155414269856
34	25.91583409022101	21.78827328691089	25.628216772630942	26.66767585023716
35	24.94197194469674	23.120395599959632	25.81996165102432	26.117670804319303
36	25.466747401352308	22.504793621959834	25.951155515188212	26.077303461499646
37	25.290140276516297	22.22222222222222	25.713997376122716	26.773640125138765
38	24.346553638106773	21.182763144616008	27.364012513876272	27.106670703400948
39	23.85205368856595	20.0827530527803	28.458976687859522	27.606216570794228
40	22.777273185992534	21.15248763750126	29.437884751236247	26.632354425269956
41	21.88919164396004	20.14330406700979	30.664042789383387	27.303461499646787
42	20.0827530527803	21.127258048238975	31.572308002825718	27.21768089615501
43	19.169441921485518	22.040569179533755	30.770007064284993	28.019981834695727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	2.5
4	3.0
5	3.0
6	2.0
7	1.0
8	1.5
9	2.0
10	2.5
11	3.0
12	3.0
13	7.0
14	11.0
15	25.5
16	40.0
17	37.5
18	35.0
19	35.0
20	42.5
21	50.0
22	44.5
23	39.0
24	48.0
25	57.0
26	57.0
27	66.0
28	75.0
29	107.5
30	140.0
31	165.5
32	191.0
33	191.0
34	228.0
35	265.0
36	315.0
37	365.0
38	438.5
39	512.0
40	512.0
41	616.0
42	720.0
43	843.5
44	967.0
45	1289.0
46	1611.0
47	1611.0
48	1782.5
49	1954.0
50	2151.0
51	2348.0
52	2374.5
53	2401.0
54	2401.0
55	2178.0
56	1955.0
57	1791.5
58	1628.0
59	1490.5
60	1353.0
61	1353.0
62	1201.0
63	1049.0
64	869.5
65	690.0
66	585.5
67	481.0
68	481.0
69	408.5
70	336.0
71	268.0
72	200.0
73	160.5
74	121.0
75	121.0
76	105.5
77	90.0
78	72.5
79	55.0
80	43.0
81	31.0
82	31.0
83	26.5
84	22.0
85	14.5
86	7.0
87	5.0
88	3.0
89	3.0
90	3.5
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	19818.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.6273085074175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.79230870538653	68.03915632253506
2	6.124061635717108	9.385407205570694
3	2.087448966153036	4.798667877686951
4	1.0338469643092323	3.1688364113432232
5	0.5268010009219017	2.018367140982945
6	0.36876070064533123	1.6954283984256735
7	0.19755037534571318	1.0596427490160458
8	0.1382852627419992	0.8477141992128367
9	0.13170025023047544	0.9082652134423252
>10	0.5992361385486632	8.078514481784236
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	49	0.24724997477041075	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	44	0.22202038550812395	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	44	0.22202038550812395	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	39	0.19679079624583712	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	35	0.17660712483600768	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	35	0.17660712483600768	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	35	0.17660712483600768	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	32	0.1614693712786356	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	32	0.1614693712786356	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	30	0.15137753557372086	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	29	0.14633161772126352	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	27	0.13623978201634876	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	26	0.13119386416389142	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	25	0.12614794631143406	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	25	0.12614794631143406	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	24	0.12110202845897669	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	24	0.12110202845897669	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23	0.11605611060651934	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	23	0.11605611060651934	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	23	0.11605611060651934	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	23	0.11605611060651934	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	21	0.1059642749016046	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	21	0.1059642749016046	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	20	0.10091835704914726	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	20	0.10091835704914726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.005045917852457362	0.0
16	0.0	0.0	0.0	0.005045917852457362	0.0
17	0.0	0.0	0.0	0.010091835704914724	0.0
18	0.0	0.0	0.0	0.010091835704914724	0.0
19	0.0	0.0	0.0	0.010091835704914724	0.0
20	0.0	0.0	0.0	0.010091835704914724	0.0
21	0.0	0.0	0.0	0.010091835704914724	0.0
22	0.0	0.0	0.0	0.015137753557372086	0.0
23	0.0	0.0	0.0	0.02018367140982945	0.0
24	0.0	0.0	0.0	0.030275507114744173	0.0
25	0.0	0.0	0.0	0.030275507114744173	0.0
26	0.0	0.0	0.0	0.03532142496720153	0.0
27	0.0	0.0	0.0	0.07568876778686043	0.0
28	0.0	0.0	0.0	0.23715813906549602	0.0
29	0.0	0.0	0.0	0.4944999495408215	0.0
30	0.005045917852457362	0.0	0.0	0.8023009385407206	0.0
31	0.005045917852457362	0.0	0.0	1.7357957412453324	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTATA	50	8.51679E-6	25.900002	37
GGTATCA	40	0.001865331	23.125	1
ATCAACG	100	2.538236E-7	18.5	2
TCAACGC	105	4.2381544E-7	17.619047	3
TATCAAC	105	4.2381544E-7	17.619047	1
CAGAGTA	110	6.9020643E-7	16.818182	9
AGAGTAC	110	6.9020643E-7	16.818182	10
GCAGAGT	110	6.9020643E-7	16.818182	8
CGCAGAG	110	6.9020643E-7	16.818182	7
CAACGCA	110	6.9020643E-7	16.818182	4
CTCTTAT	80	3.1775446E-4	16.1875	36
ACGCAGA	115	1.0987551E-6	16.086956	6
AACGCAG	125	2.620418E-6	14.799999	5
AGTACGG	90	7.779485E-4	14.388889	12
GAGTACG	90	7.779485E-4	14.388889	11
TCTCTTA	130	3.9382794E-6	14.230769	37
GTACGGG	95	0.0011711787	13.631579	13
GTCTCTT	165	4.5843273E-5	11.212121	36
CCTGTCT	165	5.8292446E-4	10.090909	37
TGTCTCT	215	6.631839E-4	8.604651	35
>>END_MODULE