FastQCFastQC Report
Fri 10 Feb 2017
ERR1631630.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631630.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158591
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC4250.2679849423990012No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3660.23078232686596342No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3610.22762956283773986No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3510.22132403478129278No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3300.20808242586275388No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT3110.19610192255550443No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3040.19168805291599145No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2830.17844644399745258No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2660.16772704630149252No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2350.14817990932650654No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2330.14691880371521712No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG2230.14061327565877005No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2220.13998272285312532No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2200.13872161724183593No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2090.1317855363797441No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT1870.11791337465556052No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG1850.11665226904427112No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1850.11665226904427112No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1820.11476061062733699No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1630.10278010732008751No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC1630.10278010732008751No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT1620.10214955451444281No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT1610.1015190017087981No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1600.10088844890315338No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG1600.10088844890315338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTGC250.00548223929.61
GGTATCA1350.027.4074081
ACCCAGT509.026715E-625.90000232
GGTGCAA1002.0008883E-1124.05000137
AATACGG1400.023.78571528
GACAGGC1103.6379788E-1223.5454547
TTGAGTA551.8892813E-523.5454542
CGGAAAC400.001923893623.12529
AAACGGA400.001923893623.12532
AACGGAG400.001923893623.12533
AACGATT400.001923893623.12522
TCCAGGA1053.8198777E-1122.9047642
TTATACA652.658704E-622.7692337
CCAGGAC1005.2386895E-1022.23
ATCAGAA1005.2386895E-1022.217
TGAGTAT502.6878915E-422.23
ATACGGA1250.022.229
CATCAGA1005.2386895E-1022.216
TACGGAG1201.0913936E-1121.58333430
TAATACG1550.021.48387127
Produced by FastQC (version 0.11.5)