##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631630.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	158591
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.589068736561345	34.0	31.0	34.0	31.0	34.0
2	32.77370090358217	34.0	31.0	34.0	31.0	34.0
3	32.86380059398074	34.0	31.0	34.0	31.0	34.0
4	36.28196429810014	37.0	37.0	37.0	35.0	37.0
5	36.20218045160192	37.0	35.0	37.0	35.0	37.0
6	36.24746675410332	37.0	37.0	37.0	35.0	37.0
7	36.238784041969595	37.0	37.0	37.0	35.0	37.0
8	36.220088151282226	37.0	37.0	37.0	35.0	37.0
9	38.058527911419944	39.0	38.0	39.0	35.0	39.0
10	37.99885239389373	39.0	38.0	39.0	35.0	39.0
11	38.064820828420274	39.0	38.0	39.0	37.0	39.0
12	38.00269876600816	39.0	38.0	39.0	35.0	39.0
13	38.057468582706456	39.0	38.0	39.0	35.0	39.0
14	39.45660850867956	40.0	39.0	41.0	37.0	41.0
15	39.45126142088769	40.0	39.0	41.0	37.0	41.0
16	39.41246981228443	40.0	39.0	41.0	37.0	41.0
17	39.37113077034636	40.0	39.0	41.0	37.0	41.0
18	39.36947872199557	40.0	39.0	41.0	36.0	41.0
19	39.37399348008399	40.0	39.0	41.0	36.0	41.0
20	39.35911873939883	40.0	39.0	41.0	36.0	41.0
21	39.33080691842538	40.0	39.0	41.0	36.0	41.0
22	39.305351501661505	40.0	39.0	41.0	36.0	41.0
23	39.24950343966555	40.0	39.0	41.0	36.0	41.0
24	39.25797806937342	40.0	39.0	41.0	36.0	41.0
25	39.189273035670375	40.0	39.0	41.0	36.0	41.0
26	39.12018336475588	40.0	39.0	41.0	36.0	41.0
27	39.02005788474756	40.0	39.0	41.0	35.0	41.0
28	39.00687302558153	40.0	39.0	41.0	35.0	41.0
29	39.005000283748764	40.0	39.0	41.0	35.0	41.0
30	38.95937348273231	40.0	38.0	41.0	35.0	41.0
31	38.892175470234754	40.0	38.0	41.0	35.0	41.0
32	38.851876840426	40.0	38.0	41.0	35.0	41.0
33	38.78992502727141	40.0	38.0	41.0	35.0	41.0
34	38.74374333978599	40.0	38.0	41.0	35.0	41.0
35	38.68616125757452	40.0	38.0	41.0	35.0	41.0
36	38.656972968201224	40.0	38.0	41.0	35.0	41.0
37	38.61779041685846	40.0	38.0	41.0	35.0	41.0
38	38.591212616100535	40.0	38.0	41.0	35.0	41.0
39	38.531890208145484	40.0	38.0	41.0	35.0	41.0
40	38.209255254081256	40.0	38.0	41.0	34.0	41.0
41	38.26427729190181	40.0	37.0	41.0	34.0	41.0
42	38.25288950823187	40.0	37.0	41.0	34.0	41.0
43	37.80631309469012	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	1.0
16	0.0
17	1.0
18	0.0
19	2.0
20	4.0
21	15.0
22	28.0
23	42.0
24	82.0
25	135.0
26	203.0
27	330.0
28	506.0
29	777.0
30	1068.0
31	1472.0
32	2023.0
33	2772.0
34	4210.0
35	6537.0
36	9348.0
37	15828.0
38	36543.0
39	76661.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.33348046232132	16.02802176668285	11.889703703236627	28.748794067759203
2	21.20990472347107	18.509877609700425	32.04532413566974	28.234893531158768
3	21.08694692637035	19.596320093826257	28.782213366458375	30.53451961334502
4	15.522318416555795	15.17488382064556	33.78943319608301	35.51336456671564
5	16.79666563676375	32.98232560485778	33.399751562194574	16.821257196183893
6	35.30528214085288	33.67025871581615	14.857085206600626	16.167373936730332
7	30.738818722373907	27.47192463632867	19.989154491742912	21.800102149554515
8	28.134635635061255	30.299953969645188	19.862413377808323	21.702997017485227
9	28.63340290432622	12.549261937940992	16.76324633806458	42.0540888196682
10	19.880068856366375	22.89915568979324	29.07920373791703	28.14157171592335
11	39.878051087388314	18.75831541512444	18.36043659476263	23.003196902724618
12	24.288894073434182	23.00761077236413	25.48505274574219	27.2184424084595
13	34.57384088630502	17.08293661052645	20.472788493672404	27.870434009496126
14	24.040456268010164	19.149888707429806	22.81592271944814	33.99373230511189
15	29.994766411713147	23.119849171768887	19.776027643434997	27.10935677308296
16	26.177399726340084	23.368286977192906	21.17017989671545	29.28413339975156
17	27.162953761562765	23.688607802460417	20.984797371855908	28.163641064120913
18	26.712739058332442	20.936244805821264	23.169032290609177	29.18198384523712
19	28.629619587492357	21.467170268174108	22.30013052443077	27.603079619902772
20	29.41654948893695	20.424866480443406	22.59207647344427	27.566507557175374
21	29.06407047058156	21.33601528460001	21.313315383596798	28.286598861221634
22	29.951258268123667	22.572529336469284	20.139856612291997	27.336355783115057
23	28.789149447320465	22.24779464156226	20.95326973157367	28.009786179543607
24	28.9278710645623	21.18342150563399	21.334123626183075	28.554583803620638
25	28.392531732569942	21.374479005744337	22.465965912315326	27.767023349370394
26	29.02812896065981	21.84613250436658	22.19987262833326	26.92586590664035
27	28.709699793809236	21.094513560038088	22.8209671418933	27.374819504259385
28	26.871007812549262	22.00944568102856	21.96278477341085	29.156761733011333
29	28.357851328259486	22.592707026249915	22.04034276850515	27.009098876985455
30	27.273300502550583	20.851750729864875	23.588980459168553	28.28596830841599
31	27.628932284934205	22.32724429507349	22.554243305105587	27.48958011488672
32	25.82870402481856	21.761638428410187	22.896633478570664	29.513024068200593
33	26.98324621195402	22.02899281800354	23.674105087930588	27.31365588211185
34	27.029907119571728	22.0044012585834	23.081385450624563	27.88430617122031
35	26.88235776305087	23.33171491446551	23.82165444445145	25.96427287803217
36	26.956132441311297	22.06619543353658	23.50448638321216	27.47318574193996
37	26.322426871638367	22.29886941881948	23.783821276112768	27.594882433429387
38	26.85209122837992	21.20990472347107	24.59975660661702	27.338247441531994
39	25.367769923892276	21.020108328972007	25.500186013077663	28.111935734058047
40	25.70448512210655	21.156307734991266	26.63328940482121	26.505917738080974
41	23.64573021167658	20.310736422621712	27.469402425106093	28.574130940595623
42	22.1651922240228	21.32403478129276	28.351545800203038	28.1592271944814
43	22.212483684446156	20.53395211581994	28.482700783777137	28.77086341595677
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	1.5
4	3.0
5	3.0
6	5.5
7	8.0
8	8.5
9	9.0
10	15.0
11	21.0
12	21.0
13	48.0
14	75.0
15	127.5
16	180.0
17	175.0
18	170.0
19	170.0
20	193.0
21	216.0
22	180.0
23	144.0
24	147.5
25	151.0
26	151.0
27	209.5
28	268.0
29	370.5
30	473.0
31	705.0
32	937.0
33	937.0
34	1345.0
35	1753.0
36	2054.0
37	2355.0
38	3101.0
39	3847.0
40	3847.0
41	4460.5
42	5074.0
43	6169.0
44	7264.0
45	8995.5
46	10727.0
47	10727.0
48	11742.0
49	12757.0
50	14688.5
51	16620.0
52	17080.0
53	17540.0
54	17540.0
55	16651.5
56	15763.0
57	14780.5
58	13798.0
59	12788.5
60	11779.0
61	11779.0
62	11398.5
63	11018.0
64	8836.5
65	6655.0
66	5831.5
67	5008.0
68	5008.0
69	4211.5
70	3415.0
71	3247.0
72	3079.0
73	3322.5
74	3566.0
75	3566.0
76	2989.5
77	2413.0
78	1630.0
79	847.0
80	567.0
81	287.0
82	287.0
83	232.0
84	177.0
85	140.0
86	103.0
87	78.5
88	54.0
89	54.0
90	39.5
91	25.0
92	16.0
93	7.0
94	4.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	158591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.11186637324943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0910549899213	41.29868655850584
2	6.74567000911577	6.6258488817145995
3	2.6474251158729953	3.9005996557181684
4	1.3468229614698215	2.6457995724851977
5	0.8846148908033433	2.1722544154460217
6	0.6714856137738004	1.978674704113096
7	0.47119544981832656	1.6198901577012568
8	0.3761860130702171	1.4780157764311972
9	0.29144786678136275	1.28821938193214
>10	2.1261571250658005	20.74455675290527
>50	0.2503627049443425	8.609568008272852
>100	0.0975772593629232	7.637886134774356
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	425	0.2679849423990012	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	366	0.23078232686596342	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	361	0.22762956283773986	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	351	0.22132403478129278	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	330	0.20808242586275388	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	311	0.19610192255550443	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	304	0.19168805291599145	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	283	0.17844644399745258	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	266	0.16772704630149252	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	235	0.14817990932650654	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	233	0.14691880371521712	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	223	0.14061327565877005	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	222	0.13998272285312532	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	220	0.13872161724183593	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	209	0.1317855363797441	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	187	0.11791337465556052	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	185	0.11665226904427112	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	185	0.11665226904427112	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	182	0.11476061062733699	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	163	0.10278010732008751	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	163	0.10278010732008751	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	162	0.10214955451444281	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	161	0.1015190017087981	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	160	0.10088844890315338	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	160	0.10088844890315338	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	6.305528056447088E-4
4	0.0	0.0	0.0	0.0	6.305528056447088E-4
5	0.0	0.0	0.0	0.0	6.305528056447088E-4
6	0.0	0.0	0.0	0.0	6.305528056447088E-4
7	0.0	0.0	0.0	0.0	6.305528056447088E-4
8	0.0	0.0	0.0	0.0	6.305528056447088E-4
9	0.0	0.0	0.0	0.0	6.305528056447088E-4
10	0.0	0.0	0.0	0.0	6.305528056447088E-4
11	0.0	0.0	0.0	0.0	6.305528056447088E-4
12	0.0	0.0	0.0	0.0	6.305528056447088E-4
13	0.0	0.0	0.0	0.0	6.305528056447088E-4
14	0.0	0.0	0.0	6.305528056447088E-4	6.305528056447088E-4
15	0.0	0.0	0.0	6.305528056447088E-4	6.305528056447088E-4
16	0.0	0.0	0.0	6.305528056447088E-4	6.305528056447088E-4
17	0.0	0.0	0.0	0.0012611056112894175	6.305528056447088E-4
18	0.0	0.0	0.0	0.0012611056112894175	6.305528056447088E-4
19	0.0	0.0	0.0	0.0018916584169341262	6.305528056447088E-4
20	0.0	0.0	0.0	0.0037833168338682523	6.305528056447088E-4
21	6.305528056447088E-4	0.0	0.0	0.007566633667736505	6.305528056447088E-4
22	6.305528056447088E-4	0.0	0.0	0.01008884489031534	6.305528056447088E-4
23	6.305528056447088E-4	0.0	0.0	0.011980503307249465	6.305528056447088E-4
24	6.305528056447088E-4	0.0	0.0	0.01513326733547301	6.305528056447088E-4
25	6.305528056447088E-4	0.0	0.0	0.018286031363696553	6.305528056447088E-4
26	6.305528056447088E-4	0.0	0.0	0.020808242586275387	6.305528056447088E-4
27	6.305528056447088E-4	0.0	0.0	0.034680404310458976	6.305528056447088E-4
28	6.305528056447088E-4	0.0	0.0	0.11917448026684994	6.305528056447088E-4
29	6.305528056447088E-4	0.0	0.0	0.22952122125467397	6.305528056447088E-4
30	6.305528056447088E-4	0.0	0.0	0.4111204292803501	6.305528056447088E-4
31	6.305528056447088E-4	0.0	0.0	0.8310685978397261	6.305528056447088E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATTGC	25	0.005482239	29.6	1
GGTATCA	135	0.0	27.407408	1
ACCCAGT	50	9.026715E-6	25.900002	32
GGTGCAA	100	2.0008883E-11	24.050001	37
AATACGG	140	0.0	23.785715	28
GACAGGC	110	3.6379788E-12	23.545454	7
TTGAGTA	55	1.8892813E-5	23.545454	2
CGGAAAC	40	0.0019238936	23.125	29
AAACGGA	40	0.0019238936	23.125	32
AACGGAG	40	0.0019238936	23.125	33
AACGATT	40	0.0019238936	23.125	22
TCCAGGA	105	3.8198777E-11	22.904764	2
TTATACA	65	2.658704E-6	22.76923	37
CCAGGAC	100	5.2386895E-10	22.2	3
ATCAGAA	100	5.2386895E-10	22.2	17
TGAGTAT	50	2.6878915E-4	22.2	3
ATACGGA	125	0.0	22.2	29
CATCAGA	100	5.2386895E-10	22.2	16
TACGGAG	120	1.0913936E-11	21.583334	30
TAATACG	155	0.0	21.483871	27
>>END_MODULE