FastQCFastQC Report
Fri 10 Feb 2017
ERR1631629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182717
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4100.2243907244536633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.20578271315750585No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3440.1882692907611224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3390.1855328185116875No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3380.1849855240618005No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3340.18279634626225255No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3170.17349234061417382No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3090.16911398501507796No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3030.16583021831575606No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG2900.15871539046722527No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2610.14284385142050274No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2560.14010737917106783No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2550.13956008472118084No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG2540.13901279027129385No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2500.13682361247174593No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC2490.13627631802185894No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2450.134087140222311No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2450.134087140222311No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC2390.1308033735229891No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT2260.12368854567445832No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA2180.11931019007536245No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA2130.11657371782592751No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2120.11602642337604054No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA2030.11110077332705769No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG2020.11055347887717071No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2020.11055347887717071No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC1940.10617512327807484No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA1910.10453323992841389No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG1890.10343865102863993No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1850.10124947322909197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACC200.001837887837.030
TTTTAAC303.5877438E-430.8333342
TTCAGAG250.005484400329.62
GGGCGTT250.005484400329.611
GCAGGTG250.005484400329.621
GGTATCA2800.028.4107131
GTGATTC604.2811735E-827.75000216
TTTAACC358.840755E-426.428573
TGATTCC659.301766E-825.61538517
GATTCCT601.3271474E-624.66666818
CAGTCGG701.9045729E-723.78571310
AGTCGGT701.9045729E-723.78571311
TCTATGG701.9045729E-723.7857132
CTCACGG400.001925010623.1253
CTCCGCT400.001925010623.12523
GCATTAG400.001925010623.12534
CTGTGCT400.001925010623.1259
GCAGTCG753.7052632E-722.29
GTCGGTG753.7052632E-722.212
TAAGTCA603.7042526E-521.58333437
Produced by FastQC (version 0.11.5)