##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631629.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	182717
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59587777820345	34.0	31.0	34.0	31.0	34.0
2	32.76114975618032	34.0	31.0	34.0	31.0	34.0
3	32.8624813235769	34.0	31.0	34.0	31.0	34.0
4	36.26786232260819	37.0	37.0	37.0	35.0	37.0
5	36.20648325005336	37.0	35.0	37.0	35.0	37.0
6	36.25055139915826	37.0	37.0	37.0	35.0	37.0
7	36.24525906182785	37.0	37.0	37.0	35.0	37.0
8	36.22295134005046	37.0	37.0	37.0	35.0	37.0
9	38.07156422226722	39.0	38.0	39.0	37.0	39.0
10	37.998117307092386	39.0	38.0	39.0	35.0	39.0
11	38.06725701494661	39.0	38.0	39.0	37.0	39.0
12	37.98964518900814	39.0	38.0	39.0	35.0	39.0
13	38.04609861151398	39.0	38.0	39.0	35.0	39.0
14	39.44168851283679	40.0	39.0	41.0	37.0	41.0
15	39.444512552198205	40.0	39.0	41.0	37.0	41.0
16	39.40899861534504	40.0	39.0	41.0	37.0	41.0
17	39.363321420557476	40.0	39.0	41.0	36.0	41.0
18	39.37947207977364	40.0	39.0	41.0	36.0	41.0
19	39.39670090905608	40.0	39.0	41.0	37.0	41.0
20	39.391687691895115	40.0	39.0	41.0	37.0	41.0
21	39.36548870657903	40.0	39.0	41.0	36.0	41.0
22	39.35914556390484	40.0	39.0	41.0	36.0	41.0
23	39.303803149132264	40.0	39.0	41.0	36.0	41.0
24	39.30901339229519	40.0	39.0	41.0	36.0	41.0
25	39.25820804851218	40.0	39.0	41.0	36.0	41.0
26	39.19815890147058	40.0	39.0	41.0	36.0	41.0
27	39.09660294334955	40.0	39.0	41.0	36.0	41.0
28	39.09214249358297	40.0	39.0	41.0	35.0	41.0
29	39.05339951947547	40.0	39.0	41.0	35.0	41.0
30	39.0058067941133	40.0	39.0	41.0	35.0	41.0
31	38.897863909762094	40.0	38.0	41.0	35.0	41.0
32	38.852613604645434	40.0	38.0	41.0	35.0	41.0
33	38.78682333882452	40.0	38.0	41.0	35.0	41.0
34	38.7443423436243	40.0	38.0	41.0	35.0	41.0
35	38.66895800609686	40.0	38.0	41.0	35.0	41.0
36	38.59568622514599	40.0	38.0	41.0	35.0	41.0
37	38.537563554567996	40.0	38.0	41.0	35.0	41.0
38	38.515316035180085	40.0	38.0	41.0	35.0	41.0
39	38.44379012352436	40.0	38.0	41.0	34.0	41.0
40	38.106716944783464	40.0	37.0	41.0	34.0	41.0
41	38.16364104051621	40.0	37.0	41.0	34.0	41.0
42	38.14168906013124	40.0	37.0	41.0	34.0	41.0
43	37.6358521648232	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	1.0
19	5.0
20	6.0
21	13.0
22	16.0
23	46.0
24	73.0
25	147.0
26	259.0
27	397.0
28	573.0
29	840.0
30	1285.0
31	1721.0
32	2472.0
33	3237.0
34	4568.0
35	6802.0
36	10608.0
37	19850.0
38	43382.0
39	86413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.606232589195315	16.469731880449	12.49801605761916	27.426019472736527
2	20.159591061587044	19.353973631353405	33.61482511205854	26.87161019500101
3	20.936201885976676	19.716829851628475	28.86649846483907	30.48046979755578
4	14.393844032027673	16.19827383330506	34.13639672280083	35.271485411866436
5	16.93438486840305	32.73094457549106	33.283164675427024	17.051505880678864
6	34.4281046645906	33.708412462989216	15.845268913127953	16.01821395929224
7	31.031048014142087	27.752754259319058	19.621053322898252	21.595144403640603
8	27.427661356086187	31.055676264387007	20.05177405495931	21.4648883245675
9	28.292386586907618	12.672602987133107	17.760799487732395	41.27421093822687
10	18.84717897075806	23.662822835313627	29.99337773715637	27.496620456771947
11	39.21638380665182	19.807680730309716	18.520444184175528	22.45549127886294
12	22.710530492510276	23.778849258689668	26.559652358565433	26.950967890234622
13	34.739515206576286	17.56924643027195	21.31438235084858	26.37685601230318
14	24.34475171987281	19.940125987182363	23.599336679126736	32.11578561381809
15	28.740620741365063	24.247333307792925	21.159498021530567	25.85254792931145
16	26.238937810931663	23.69620779675673	22.73187497605587	27.332979416255736
17	26.813049688863106	23.646951296266906	21.85729844513647	27.68270056973352
18	26.511490446975376	21.34831460674157	23.97696984954876	28.163225096734294
19	28.839681036794605	22.64430786407395	23.324047570833585	25.191963528297862
20	30.073282726839867	20.950978836123625	22.923975327966197	26.051763109070308
21	26.721104221282094	22.864867527378404	22.416633372920966	27.997394878418536
22	28.57205405079987	22.570423113339206	21.889041523229913	26.96848131263101
23	26.50930126917583	22.296775888395715	23.289020726040818	27.904902116387635
24	27.178095086937727	22.31593119414176	23.163690297016696	27.34228342190382
25	27.335715888505174	22.33016084983882	23.623964929371652	26.71015833228435
26	27.793801343060583	22.925069916865972	22.325782494239725	26.955346245833724
27	27.248148776523255	22.122188958881768	23.783227614288762	26.84643465030621
28	26.223613566334826	23.37166218797375	22.5917675968848	27.812956648806626
29	27.569410618606916	23.762430425193056	23.053684112589416	25.614474843610612
30	27.2125746372806	21.84799443948839	23.452661766557025	27.486769156673986
31	26.67622607639136	22.10358094758561	23.55828959538521	27.661903380637813
32	24.99822129303787	22.629530913927002	23.60645150697527	28.765796286059864
33	25.89961525200173	21.60663758708823	25.419638019450847	27.074109141459196
34	26.66254371514418	21.88247398983127	24.76343197403635	26.691550320988195
35	26.264660650076348	22.810685376839594	24.371021853467383	26.553632119616676
36	25.55317786522327	21.892325289929236	24.802289879978325	27.75220696486917
37	25.361624807762823	21.65917785427738	26.001959314130595	26.9772380238292
38	25.21494989519311	20.85848607409272	25.58875200446592	28.337812026248244
39	24.254995430091345	20.7752973177099	28.011624534115597	26.958082718083155
40	25.31948313512153	21.070289026198985	27.236655593075632	26.373572245603853
41	22.612564785980503	20.832763234948036	28.8987888373824	27.65588314168906
42	21.13103871013644	22.093182353037758	29.238658690762215	27.537120246063584
43	21.280450094955587	21.845257967238954	28.60543901224298	28.26885292556248
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	9.5
4	17.0
5	17.0
6	22.5
7	28.0
8	35.5
9	43.0
10	62.5
11	82.0
12	82.0
13	168.5
14	255.0
15	458.0
16	661.0
17	600.0
18	539.0
19	539.0
20	612.0
21	685.0
22	566.5
23	448.0
24	466.0
25	484.0
26	484.0
27	544.5
28	605.0
29	756.0
30	907.0
31	1162.5
32	1418.0
33	1418.0
34	2076.5
35	2735.0
36	3036.5
37	3338.0
38	3867.0
39	4396.0
40	4396.0
41	5188.5
42	5981.0
43	7197.0
44	8413.0
45	10991.5
46	13570.0
47	13570.0
48	14838.5
49	16107.0
50	17978.0
51	19849.0
52	20508.5
53	21168.0
54	21168.0
55	19317.0
56	17466.0
57	15819.5
58	14173.0
59	13778.0
60	13383.0
61	13383.0
62	12318.0
63	11253.0
64	8924.0
65	6595.0
66	5947.0
67	5299.0
68	5299.0
69	4706.0
70	4113.0
71	4173.0
72	4233.0
73	3498.0
74	2763.0
75	2763.0
76	1736.5
77	710.0
78	558.0
79	406.0
80	329.5
81	253.0
82	253.0
83	207.0
84	161.0
85	127.0
86	93.0
87	70.5
88	48.0
89	48.0
90	39.0
91	30.0
92	18.0
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	182717.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.31104385470427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.882202359366	44.067054516000155
2	6.047741963009578	6.206319061718395
3	2.330567229131557	3.5875151190091783
4	1.2447468907993258	2.55477049207244
5	0.7967660046504682	2.044144770327884
6	0.5642425923160612	1.7371125839412864
7	0.4159822514239392	1.4941138481914655
8	0.32425283187917314	1.3310201021251444
9	0.22719030654692066	1.0491634604333477
>10	1.8569874352027647	18.97415128313184
>50	0.1919918083495104	7.0026324863039555
>100	0.11732832732470082	9.952002276744912
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	410	0.2243907244536633	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	376	0.20578271315750585	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	344	0.1882692907611224	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	339	0.1855328185116875	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	338	0.1849855240618005	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	334	0.18279634626225255	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	317	0.17349234061417382	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	309	0.16911398501507796	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	303	0.16583021831575606	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	290	0.15871539046722527	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	261	0.14284385142050274	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	256	0.14010737917106783	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	255	0.13956008472118084	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	254	0.13901279027129385	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	250	0.13682361247174593	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	249	0.13627631802185894	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	245	0.134087140222311	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	245	0.134087140222311	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	239	0.1308033735229891	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	226	0.12368854567445832	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	218	0.11931019007536245	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	213	0.11657371782592751	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	212	0.11602642337604054	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	203	0.11110077332705769	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	202	0.11055347887717071	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	202	0.11055347887717071	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	194	0.10617512327807484	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	191	0.10453323992841389	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	189	0.10343865102863993	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	185	0.10124947322909197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.472944498869837E-4	0.0	0.0	0.0
6	0.0	5.472944498869837E-4	0.0	0.0	0.0
7	0.0	5.472944498869837E-4	0.0	0.0	0.0
8	0.0	5.472944498869837E-4	0.0	0.0	0.0
9	0.0	0.0010945888997739674	0.0	0.0	0.0
10	0.0	0.0010945888997739674	0.0	0.0	0.0
11	0.0	0.0010945888997739674	0.0	0.0	0.0
12	0.0	0.0010945888997739674	0.0	5.472944498869837E-4	0.0
13	0.0	0.0010945888997739674	0.0	5.472944498869837E-4	0.0
14	0.0	0.0010945888997739674	0.0	0.001641883349660951	0.0
15	0.0	0.0010945888997739674	0.0	0.002189177799547935	0.0
16	0.0	0.0010945888997739674	0.0	0.003831061149208886	0.0
17	0.0	0.0010945888997739674	0.0	0.006567533398643804	0.0
18	0.0	0.0010945888997739674	0.0	0.007662122298417772	0.0
19	0.0	0.0010945888997739674	0.0	0.007662122298417772	0.0
20	0.0	0.0010945888997739674	0.0	0.010398594547852691	0.0
21	0.0	0.0010945888997739674	0.0	0.016966127946496493	0.0
22	0.0	0.0010945888997739674	0.0	0.02517554469480125	0.0
23	0.0	0.0010945888997739674	0.0	0.029006605844010137	0.0
24	0.0	0.0010945888997739674	0.0	0.03557413924265394	0.0
25	0.0	0.0010945888997739674	0.0	0.04104708374152378	0.0
26	0.0	0.0010945888997739674	0.0	0.04433085044084568	0.0
27	0.0	0.0010945888997739674	0.0	0.06786451178598597	0.0
28	0.0	0.0010945888997739674	0.0	0.17622881286360875	0.0
29	0.0	0.0010945888997739674	0.0	0.3519103312773305	0.0
30	0.0	0.0010945888997739674	0.0	0.6239156728711615	0.0
31	0.0	0.0010945888997739674	0.0	1.5067016205388661	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGACC	20	0.0018378878	37.0	30
TTTTAAC	30	3.5877438E-4	30.833334	2
TTCAGAG	25	0.0054844003	29.6	2
GGGCGTT	25	0.0054844003	29.6	11
GCAGGTG	25	0.0054844003	29.6	21
GGTATCA	280	0.0	28.410713	1
GTGATTC	60	4.2811735E-8	27.750002	16
TTTAACC	35	8.840755E-4	26.42857	3
TGATTCC	65	9.301766E-8	25.615385	17
GATTCCT	60	1.3271474E-6	24.666668	18
CAGTCGG	70	1.9045729E-7	23.785713	10
AGTCGGT	70	1.9045729E-7	23.785713	11
TCTATGG	70	1.9045729E-7	23.785713	2
CTCACGG	40	0.0019250106	23.125	3
CTCCGCT	40	0.0019250106	23.125	23
GCATTAG	40	0.0019250106	23.125	34
CTGTGCT	40	0.0019250106	23.125	9
GCAGTCG	75	3.7052632E-7	22.2	9
GTCGGTG	75	3.7052632E-7	22.2	12
TAAGTCA	60	3.7042526E-5	21.583334	37
>>END_MODULE