FastQCFastQC Report
Fri 10 Feb 2017
ERR1631628.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631628.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868220
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA41360.22138720279196242No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA40600.21731915941377353No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA40390.21619509479611607No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA38840.20789842738007303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38350.2052756099388723No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC36100.19323206046397104No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA33240.1779233709092077No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG31180.16689683227885366No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA31040.16614745586708204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30910.16545160634186554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29780.15940306816113733No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT29680.15886779929558617No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC28090.15035702433332263No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA27310.14618192718202352No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA26690.1428632602156063No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT23950.12819689329950434No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG23320.12482469944653199No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC22900.1225765702112171No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT20860.1116570853539733No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA20640.11047949384976075No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA20420.10930190234554817No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA19580.10480564387491838No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18880.1010587618160602No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC18820.10073760049672952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24300.029.3868331
TCTAGTA454.010022E-628.7777792
ATACGGC3350.023.1940329
TATACCG400.001931636823.1255
GCGGTAA16450.020.01823823
GTTAGAT656.905082E-519.92307927
CTCTAGT759.2708615E-619.7333341
TTAGACT951.6775084E-719.4736844
TTTTACG1052.2617314E-819.380954
CTTATAC18300.019.30874337
TACGGCT4050.019.18518430
CTTAGGA4650.019.0967752
CGCGGTA17550.018.86894622
CTCTATG8350.018.8323351
CGTTATT1501.2732926E-1118.510
TAGGACA3000.018.54
TTAGGAC6300.018.2063483
TCTATGG8750.017.9714282
TTTAGGA1451.546141E-1017.8620682
AACTCCG18650.017.8552285
Produced by FastQC (version 0.11.5)