##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631628.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1868220
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61642793675263	34.0	31.0	34.0	31.0	34.0
2	32.789588485296164	34.0	31.0	34.0	31.0	34.0
3	32.87819796383724	34.0	31.0	34.0	31.0	34.0
4	36.28396388005695	37.0	37.0	37.0	35.0	37.0
5	36.21079958463136	37.0	35.0	37.0	35.0	37.0
6	36.269211334853495	37.0	37.0	37.0	35.0	37.0
7	36.263201871299955	37.0	37.0	37.0	35.0	37.0
8	36.244228195822764	37.0	37.0	37.0	35.0	37.0
9	38.07654451831155	39.0	38.0	39.0	37.0	39.0
10	38.01234330003961	39.0	38.0	39.0	35.0	39.0
11	38.080029118626285	39.0	38.0	39.0	37.0	39.0
12	38.0201202213872	39.0	38.0	39.0	35.0	39.0
13	38.069999785892456	39.0	38.0	39.0	37.0	39.0
14	39.46790741989702	40.0	39.0	41.0	37.0	41.0
15	39.46829548982454	41.0	39.0	41.0	37.0	41.0
16	39.42449979124514	40.0	39.0	41.0	37.0	41.0
17	39.39506267998416	40.0	39.0	41.0	37.0	41.0
18	39.42324351521769	41.0	39.0	41.0	37.0	41.0
19	39.44794242648082	41.0	39.0	41.0	37.0	41.0
20	39.44101979424265	41.0	39.0	41.0	37.0	41.0
21	39.40610688248707	40.0	39.0	41.0	37.0	41.0
22	39.38501943025982	40.0	39.0	41.0	37.0	41.0
23	39.32646369271285	40.0	39.0	41.0	36.0	41.0
24	39.339821862521546	40.0	39.0	41.0	36.0	41.0
25	39.287445804027364	40.0	39.0	41.0	36.0	41.0
26	39.228354262345974	40.0	39.0	41.0	36.0	41.0
27	39.119477363479675	40.0	39.0	41.0	36.0	41.0
28	39.11432593591761	40.0	39.0	41.0	36.0	41.0
29	39.10106893192451	40.0	39.0	41.0	35.0	41.0
30	39.05655757887187	40.0	39.0	41.0	35.0	41.0
31	38.9724106368629	40.0	38.0	41.0	35.0	41.0
32	38.926396784104654	40.0	38.0	41.0	35.0	41.0
33	38.874813458800354	40.0	38.0	41.0	35.0	41.0
34	38.84596032587169	40.0	38.0	41.0	35.0	41.0
35	38.77489267859246	40.0	38.0	41.0	35.0	41.0
36	38.72984070398561	40.0	38.0	41.0	35.0	41.0
37	38.69017192835962	40.0	38.0	41.0	35.0	41.0
38	38.64725942340838	40.0	38.0	41.0	35.0	41.0
39	38.57563830812217	40.0	38.0	41.0	35.0	41.0
40	38.26974660371905	40.0	38.0	41.0	34.0	41.0
41	38.34043046322168	40.0	38.0	41.0	34.0	41.0
42	38.32526201410969	40.0	38.0	41.0	34.0	41.0
43	37.860208647803795	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	7.0
12	2.0
13	1.0
14	1.0
15	2.0
16	4.0
17	8.0
18	16.0
19	18.0
20	72.0
21	143.0
22	259.0
23	538.0
24	894.0
25	1455.0
26	2368.0
27	3723.0
28	5619.0
29	8612.0
30	12181.0
31	16858.0
32	22530.0
33	31136.0
34	44934.0
35	67685.0
36	104525.0
37	192159.0
38	436191.0
39	916273.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.62902656004111	16.913853828778194	12.941570050636434	27.51554956054426
2	19.643885623748808	20.028208669214546	34.11322007044138	26.214685636595263
3	20.357345494641958	20.701523375191357	30.05711318795431	28.884017942212374
4	14.650576484568198	16.442067850681397	35.182794317585724	33.72456134716468
5	15.723576452452068	34.079069916819215	34.28332851591354	15.914025114815173
6	32.529145389729266	35.3436961385704	16.180481956086542	15.946676515613792
7	29.441125777478028	28.996906145957112	20.70077399877959	20.86119407778527
8	26.602594983460193	32.148301591889606	20.633704809926027	20.615398614724175
9	28.083041611801608	13.493432251019687	18.154500005352688	40.26902613182602
10	18.474911948271615	24.36720514714541	30.539015747610026	26.618867156972946
11	37.932042264829626	20.43394246930233	19.847180738885143	21.786834526982904
12	21.993983577951205	24.563060025050586	27.767821776878527	25.67513462011969
13	33.72349080943358	18.380544047274945	22.299461519521255	25.596503623770218
14	23.37513783173288	20.5581248461102	24.419875603515646	31.646861718641272
15	28.432946869212405	25.08093265247134	21.198413463082506	25.28770701523375
16	24.48319791031035	25.202920426930447	23.294526340580873	27.019355322178328
17	25.796587125713245	24.828499855477407	22.717078288424275	26.657834730385073
18	25.649548768346342	22.3972016143709	24.548340131247926	27.404909486034835
19	27.269325882390728	23.200158439584204	24.534209033197374	24.996306644827698
20	28.838948303732963	21.685133442528183	24.018798642558156	25.457119611180694
21	26.24353662844847	23.338686021988845	23.127897142734795	27.28988020682789
22	27.029686011283466	23.726702422626886	22.687049705066855	26.556561861022793
23	26.699371594351845	23.207384569269145	23.872402607829912	26.2208412285491
24	26.701833831133374	22.765680701416322	24.008093265247133	26.524392202203167
25	26.84571410219353	23.223442635235678	24.33225209022492	25.59859117234587
26	26.832760595647194	23.69736968879468	23.557450407339605	25.91241930821852
27	26.179197310809222	22.794585220156087	24.883793129288843	26.142424339745858
28	25.38036205586066	23.9632912612005	23.478872937876698	27.177473745062148
29	26.21827193799445	24.39605613899862	23.42807592253589	25.95759600047104
30	25.897003564890646	23.49503805761634	24.58875293059704	26.019205446895977
31	26.70756120799477	23.494556315637343	23.916027020372333	25.881855455995545
32	24.343278628855273	23.598237894894606	24.202663497874983	27.85581997837514
33	24.82833927481774	23.06350429820899	25.78577469462911	26.32238173234416
34	25.45594201967648	22.977754225947695	24.98597595572256	26.580327798653265
35	24.93196732718845	24.485338985772554	25.29803770433889	25.28465598270011
36	25.244724925329994	23.451360118187363	25.61015297984178	25.693761976640868
37	26.131023112909613	23.040969478969288	24.894605560372977	25.933401847748122
38	25.09666955711854	22.627206645898234	25.896735930457872	26.379387866525356
39	24.857832589309613	21.621757608846924	26.69144961514169	26.828960186701778
40	24.49690079326846	22.51469313035938	27.385586279988438	25.602819796383724
41	22.870218710858463	21.845018252668318	28.402168909443215	26.882594127030007
42	21.975463275203136	22.140861354658444	28.89590091102761	26.987774459110813
43	21.200286904111934	22.888953121152756	28.856398068749932	27.054361905985374
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	24.5
2	26.0
3	82.5
4	139.0
5	139.0
6	213.0
7	287.0
8	318.0
9	349.0
10	567.5
11	786.0
12	786.0
13	1446.5
14	2107.0
15	4427.5
16	6748.0
17	6623.5
18	6499.0
19	6499.0
20	7150.0
21	7801.0
22	7071.5
23	6342.0
24	7029.0
25	7716.0
26	7716.0
27	8792.5
28	9869.0
29	13428.5
30	16988.0
31	20151.0
32	23314.0
33	23314.0
34	28390.0
35	33466.0
36	36690.5
37	39915.0
38	49000.5
39	58086.0
40	58086.0
41	68438.0
42	78790.0
43	87339.0
44	95888.0
45	124057.0
46	152226.0
47	152226.0
48	164954.0
49	177682.0
50	195855.5
51	214029.0
52	216526.5
53	219024.0
54	219024.0
55	193335.5
56	167647.0
57	154551.5
58	141456.0
59	128653.0
60	115850.0
61	115850.0
62	105193.0
63	94536.0
64	77510.0
65	60484.0
66	52053.5
67	43623.0
68	43623.0
69	37156.5
70	30690.0
71	26199.0
72	21708.0
73	17581.5
74	13455.0
75	13455.0
76	10861.5
77	8268.0
78	6778.0
79	5288.0
80	4197.0
81	3106.0
82	3106.0
83	2486.0
84	1866.0
85	1461.5
86	1057.0
87	870.5
88	684.0
89	684.0
90	502.0
91	320.0
92	193.0
93	66.0
94	40.5
95	15.0
96	15.0
97	8.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1868220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.606306515220226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14051216062265	33.72101221496456
2	7.187459040827375	5.693374116731995
3	2.320980670206998	2.7577641552035885
4	1.1334708085846197	1.7957036908342783
5	0.7096471469383002	1.4053251209644921
6	0.5009599200147723	1.1904703286367166
7	0.3493110940205155	0.9684445581260415
8	0.287465294866151	0.9108350864765552
9	0.20619249658480449	0.7349870898788639
>10	1.667617892606128	13.348158988667835
>50	0.24212400345502597	6.810634176554112
>100	0.22318944250333253	17.300814858895023
>500	0.0200801932657656	5.4439516440116
>1k	0.010989835503560903	7.918523970054303
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	4136	0.22138720279196242	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	4060	0.21731915941377353	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	4039	0.21619509479611607	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3884	0.20789842738007303	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3835	0.2052756099388723	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	3610	0.19323206046397104	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	3324	0.1779233709092077	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	3118	0.16689683227885366	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	3104	0.16614745586708204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3091	0.16545160634186554	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2978	0.15940306816113733	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	2968	0.15886779929558617	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	2809	0.15035702433332263	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2731	0.14618192718202352	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	2669	0.1428632602156063	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	2395	0.12819689329950434	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	2332	0.12482469944653199	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	2290	0.1225765702112171	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	2086	0.1116570853539733	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	2064	0.11047949384976075	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	2042	0.10930190234554817	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1958	0.10480564387491838	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1888	0.1010587618160602	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1882	0.10073760049672952	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.3526886555116634E-5	0.0
5	0.0	0.0	0.0	5.3526886555116634E-5	0.0
6	0.0	0.0	0.0	5.3526886555116634E-5	0.0
7	0.0	0.0	0.0	5.3526886555116634E-5	0.0
8	0.0	0.0	0.0	5.3526886555116634E-5	0.0
9	0.0	0.0	0.0	5.3526886555116634E-5	0.0
10	0.0	0.0	0.0	5.3526886555116634E-5	0.0
11	0.0	0.0	0.0	5.3526886555116634E-5	0.0
12	0.0	0.0	0.0	1.0705377311023327E-4	0.0
13	0.0	0.0	0.0	1.6058065966534992E-4	0.0
14	1.0705377311023327E-4	0.0	0.0	2.6763443277558316E-4	0.0
15	1.0705377311023327E-4	0.0	0.0	6.958495252165162E-4	0.0
16	1.0705377311023327E-4	0.0	0.0	0.0012311183907676825	0.0
17	1.6058065966534992E-4	0.0	0.0	0.0027298712143109484	0.0
18	2.1410754622046654E-4	0.0	0.0	0.0031045594201967648	0.0
19	2.1410754622046654E-4	0.0	0.0	0.003960989605078631	0.0
20	2.1410754622046654E-4	0.0	0.0	0.0057273768613974796	0.0
21	2.6763443277558316E-4	0.0	0.0	0.008671355621928894	0.0
22	2.6763443277558316E-4	0.0	0.0	0.012632345227007525	0.0
23	2.6763443277558316E-4	0.0	0.0	0.01509458200854289	0.0
24	2.6763443277558316E-4	5.3526886555116634E-5	0.0	0.018841464067401056	0.0
25	2.6763443277558316E-4	5.3526886555116634E-5	0.0	0.02066137821027502	0.0
26	2.6763443277558316E-4	5.3526886555116634E-5	0.0	0.023765937630471787	0.0
27	2.6763443277558316E-4	5.3526886555116634E-5	0.0	0.03709413238269583	0.0
28	2.6763443277558316E-4	5.3526886555116634E-5	0.0	0.1039492136900365	0.0
29	2.6763443277558316E-4	5.3526886555116634E-5	0.0	0.22074488015330102	0.0
30	3.2116131933069983E-4	5.3526886555116634E-5	0.0	0.3732965068353834	0.0
31	3.2116131933069983E-4	5.3526886555116634E-5	0.0	0.854021474986886	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2430	0.0	29.386833	1
TCTAGTA	45	4.010022E-6	28.777779	2
ATACGGC	335	0.0	23.19403	29
TATACCG	40	0.0019316368	23.125	5
GCGGTAA	1645	0.0	20.018238	23
GTTAGAT	65	6.905082E-5	19.923079	27
CTCTAGT	75	9.2708615E-6	19.733334	1
TTAGACT	95	1.6775084E-7	19.473684	4
TTTTACG	105	2.2617314E-8	19.38095	4
CTTATAC	1830	0.0	19.308743	37
TACGGCT	405	0.0	19.185184	30
CTTAGGA	465	0.0	19.096775	2
CGCGGTA	1755	0.0	18.868946	22
CTCTATG	835	0.0	18.832335	1
CGTTATT	150	1.2732926E-11	18.5	10
TAGGACA	300	0.0	18.5	4
TTAGGAC	630	0.0	18.206348	3
TCTATGG	875	0.0	17.971428	2
TTTAGGA	145	1.546141E-10	17.862068	2
AACTCCG	1865	0.0	17.855228	5
>>END_MODULE