FastQCFastQC Report
Fri 10 Feb 2017
ERR1631627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619760
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20740.3346456692913386No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA19770.31899444946430877No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC17940.28946689040919066No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17640.28462630695753194No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17530.28285142635859045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16810.27123402607460956No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16740.27010455660255583No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG15720.2536465728669162No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14400.2323480056796179No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14130.2279914805731251No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14000.22589389441073962No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC13920.22460307215696398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13120.21169484961920745No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12760.20588614947721698No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12740.20556344391377307No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11370.18345811281786498No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA9860.1590938427778495No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9460.15263973150897123No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9280.149735381437976No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8820.14231315347876597No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8800.14199044791532206No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8780.14166774235187815No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8590.13860203949916097No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8540.1377952755905512No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8430.13602039499160964No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8430.13602039499160964No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT8340.13456821995611204No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7710.12440299470762876No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT7590.12246676132696528No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA7510.12117593907318963No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT7470.12053052794630179No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7390.11923970569252614No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG7360.11875564734736027No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT7300.11778753065702853No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7220.11649670840325288No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7170.1156899444946431No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7120.1148831805860333No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7070.1140764166774235No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA7050.1137537111139796No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC7010.11310829998709176No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6940.11197883051503807No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6730.10859042209887698No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6640.10713824706337938No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6560.10584742480960373No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6500.10487930811927197No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6380.10294307473860849No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC6320.10197495804827675No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6260.10100684135794501No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6220.10036143023105719No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG6200.10003872466761328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.028.0421071
ATTAGAG601.3357458E-624.6666663
TAATACT851.9717845E-923.9411754
ATACGGC1700.023.94117529
TTTTACG701.9199433E-723.7857134
TCTATCG551.900825E-523.54545431
TTTACTT400.00193018823.12500223
GTGCTAT400.00193018823.1250021
CTATATC652.679535E-622.769234
ATATCAG652.679535E-622.769235
TATCTAT652.679535E-622.769237
GCGGTAA7650.022.24836523
TATATCT705.095666E-621.1428575
TATTGCG705.095666E-621.14285711
ACTGTGC1059.804353E-1021.1428578
ACTATAT806.949467E-720.81253
CTTTTTA806.949467E-720.81252
CTAAGTC806.949467E-720.812534
GTACTAT806.949467E-720.81251
GCTTTTT806.949467E-720.81251
Produced by FastQC (version 0.11.5)