##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631627.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619760
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.567203433587196	34.0	31.0	34.0	31.0	34.0
2	32.733125726087515	34.0	31.0	34.0	31.0	34.0
3	32.817121143668516	34.0	31.0	34.0	31.0	34.0
4	36.23449722473215	37.0	37.0	37.0	35.0	37.0
5	36.1726668387763	37.0	35.0	37.0	35.0	37.0
6	36.239152252484836	37.0	37.0	37.0	35.0	37.0
7	36.23446818123144	37.0	37.0	37.0	35.0	37.0
8	36.21218697560346	37.0	36.0	37.0	35.0	37.0
9	38.040097779785725	39.0	38.0	39.0	35.0	39.0
10	37.97183909900607	39.0	38.0	39.0	35.0	39.0
11	38.045790305924875	39.0	38.0	39.0	35.0	39.0
12	37.96990447915322	39.0	38.0	39.0	35.0	39.0
13	38.03486833613012	39.0	38.0	39.0	35.0	39.0
14	39.39290531818769	40.0	39.0	41.0	37.0	41.0
15	39.401915257519036	40.0	39.0	41.0	37.0	41.0
16	39.33904898670453	40.0	39.0	41.0	36.0	41.0
17	39.31944462372531	40.0	39.0	41.0	36.0	41.0
18	39.372174712792045	40.0	39.0	41.0	36.0	41.0
19	39.38677391248225	40.0	39.0	41.0	37.0	41.0
20	39.387963082483544	40.0	39.0	41.0	37.0	41.0
21	39.34822995998451	40.0	39.0	41.0	36.0	41.0
22	39.31480734477862	40.0	39.0	41.0	36.0	41.0
23	39.26436523815671	40.0	39.0	41.0	36.0	41.0
24	39.27463050212986	40.0	39.0	41.0	36.0	41.0
25	39.22379630824835	40.0	39.0	41.0	36.0	41.0
26	39.151444107396415	40.0	39.0	41.0	36.0	41.0
27	39.030984574674065	40.0	39.0	41.0	35.0	41.0
28	39.03869723764038	40.0	39.0	41.0	35.0	41.0
29	39.01806989802504	40.0	39.0	41.0	35.0	41.0
30	38.964434619852845	40.0	38.0	41.0	35.0	41.0
31	38.87759455273009	40.0	38.0	41.0	35.0	41.0
32	38.838437459661804	40.0	38.0	41.0	35.0	41.0
33	38.77215050987479	40.0	38.0	41.0	35.0	41.0
34	38.75191041693559	40.0	38.0	41.0	35.0	41.0
35	38.67276849102878	40.0	38.0	41.0	35.0	41.0
36	38.62948399380405	40.0	38.0	41.0	35.0	41.0
37	38.58808571059765	40.0	38.0	41.0	35.0	41.0
38	38.556044275203305	40.0	38.0	41.0	35.0	41.0
39	38.47360107138247	40.0	38.0	41.0	35.0	41.0
40	38.15328191558022	40.0	38.0	41.0	34.0	41.0
41	38.228845036788435	40.0	37.0	41.0	34.0	41.0
42	38.20041629017684	40.0	37.0	41.0	34.0	41.0
43	37.72961307602943	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	0.0
14	0.0
15	1.0
16	2.0
17	4.0
18	2.0
19	15.0
20	16.0
21	37.0
22	88.0
23	139.0
24	272.0
25	481.0
26	857.0
27	1301.0
28	2050.0
29	2946.0
30	4235.0
31	5851.0
32	7926.0
33	10954.0
34	15918.0
35	23754.0
36	36108.0
37	66992.0
38	152168.0
39	287638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.080966825868074	17.01852329934168	13.4266490254292	27.473860849361042
2	20.24977410610559	20.026623208984123	33.67013037304763	26.053472311862656
3	20.11375371111398	20.578611075254937	30.674938685942944	28.63269652768814
4	13.803730476313412	17.297663611720665	36.95607977281528	31.94252613915064
5	15.330773202530013	33.89925132309281	35.03210920356267	15.73786627081451
6	30.259293920227186	36.84442364786369	16.7019491416032	16.194333290305927
7	28.322415128436813	29.189524977410606	21.106880082612626	21.38117981153995
8	26.12236994965793	31.224828966051376	21.303407770749967	21.349393313540727
9	28.387278946689044	12.68555569898025	18.59897379630825	40.32819155802246
10	19.058667871434103	23.411320511165613	29.683425842261517	27.846585775138767
11	38.30934555311734	20.25461468955725	19.208241900090357	22.22779785723506
12	21.621434103523946	25.403704659868335	27.219730218148964	25.75513101845876
13	34.98709177746224	18.37808183813089	21.238705305279463	25.396121079127404
14	22.882567445462758	21.622563572995997	24.92593907318962	30.56892990835162
15	29.467858525880985	24.66535433070866	21.440718987995353	24.426068155415
16	23.404059635988126	25.316412804956755	24.108203175422744	27.171324383632374
17	25.763682715890024	24.787014328127015	22.42174390086485	27.02755905511811
18	25.825964889634694	21.7842390602814	24.24244868981541	28.147347360268494
19	27.187621014586295	22.959048663998967	24.585000645411125	25.268329676003614
20	29.93739512069188	20.732541629017685	23.940073576868464	25.38998967342197
21	26.256615464050597	23.020846779398475	23.041499935458887	27.681037821092037
22	27.729282302826903	23.851652252484833	22.348167032399637	26.070898412288628
23	27.221182393184456	22.864011875564735	23.653026978185103	26.261778753065702
24	26.591422486123662	22.364463663353558	24.304569510778364	26.73954433974442
25	26.744546275977797	22.684748935071642	24.802342842390605	25.76836194655996
26	26.635149089970312	23.982993416806504	23.752258938944106	25.629598554279077
27	25.70559571447012	22.657318962178906	26.067187298309026	25.569898025041955
28	24.903349683748548	24.09884471408287	23.45730605395637	27.54049954821221
29	26.43055376274687	24.584677939847683	23.59719891570931	25.38756938169614
30	25.84016393442623	22.4603072156964	25.30995869368788	26.38957015618949
31	26.81973667226023	23.471021040402736	24.195333677552604	25.51390860978443
32	24.149348134761844	23.1959145475668	24.345553117335744	28.309184200335615
33	24.26358590422099	22.53549761197883	26.882986962695238	26.317929521104944
34	25.12811410868723	22.57486769071899	25.883729185491156	26.41328901510262
35	24.171937524202917	24.778623983477473	26.16835549244869	24.88108299987092
36	24.851071382470632	23.50474377178263	26.361817477733318	25.282367368013425
37	25.33061184974829	23.292887569381694	25.895023880211692	25.481476700658316
38	24.687136956241126	22.093068284497225	26.506066864592746	26.713727894668903
39	24.048663998967342	21.072511940105848	27.988092164708924	26.89073189621789
40	23.74661159158384	22.488705305279463	28.504259713437456	25.260423389699238
41	21.991738737575837	21.329224215825484	29.536917516457983	27.1421195301407
42	20.20637020782238	22.216503162514524	30.769330063250287	26.807796566412804
43	19.613882793339357	22.876436039757326	30.093907318962177	27.41577384794114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	10.5
2	13.0
3	34.0
4	55.0
5	55.0
6	67.5
7	80.0
8	106.0
9	132.0
10	196.0
11	260.0
12	260.0
13	545.0
14	830.0
15	1798.5
16	2767.0
17	2639.5
18	2512.0
19	2512.0
20	2699.0
21	2886.0
22	2273.5
23	1661.0
24	1684.5
25	1708.0
26	1708.0
27	1886.5
28	2065.0
29	2543.0
30	3021.0
31	3928.5
32	4836.0
33	4836.0
34	6793.0
35	8750.0
36	9678.5
37	10607.0
38	13440.0
39	16273.0
40	16273.0
41	19616.5
42	22960.0
43	27759.0
44	32558.0
45	44965.5
46	57373.0
47	57373.0
48	63498.0
49	69623.0
50	76532.5
51	83442.0
52	81061.0
53	78680.0
54	78680.0
55	68576.5
56	58473.0
57	53025.0
58	47577.0
59	43240.0
60	38903.0
61	38903.0
62	33645.5
63	28388.0
64	21961.0
65	15534.0
66	12927.0
67	10320.0
68	10320.0
69	8626.0
70	6932.0
71	5732.0
72	4532.0
73	3441.0
74	2350.0
75	2350.0
76	1859.0
77	1368.0
78	1118.0
79	868.0
80	744.5
81	621.0
82	621.0
83	503.0
84	385.0
85	287.5
86	190.0
87	165.5
88	141.0
89	141.0
90	97.0
91	53.0
92	35.5
93	18.0
94	12.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	619760.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.16136587896955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32778613362174	28.947574102498297
2	7.0667237179506435	4.969513164249091
3	2.794255305406696	2.9474949945796993
4	1.5817102433001897	2.2246037031676766
5	1.0200257613637649	1.793274950064291
6	0.7343663127629956	1.5492793567349683
7	0.5321657626103489	1.309817256118233
8	0.4148092417141901	1.1668207614312442
9	0.32468779730155195	1.0274819793620915
>10	2.5634159870767714	17.90237450356573
>50	0.35702419427757454	8.70286385268687
>100	0.2501944159377893	17.259866926663662
>500	0.025435661509387083	6.144690195535894
>1k	0.007399465166367151	4.0543442533422365
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2074	0.3346456692913386	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1977	0.31899444946430877	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1794	0.28946689040919066	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1764	0.28462630695753194	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1753	0.28285142635859045	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1681	0.27123402607460956	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1674	0.27010455660255583	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1572	0.2536465728669162	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1440	0.2323480056796179	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1413	0.2279914805731251	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1400	0.22589389441073962	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1392	0.22460307215696398	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1312	0.21169484961920745	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1276	0.20588614947721698	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1274	0.20556344391377307	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1137	0.18345811281786498	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	986	0.1590938427778495	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	946	0.15263973150897123	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	928	0.149735381437976	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	882	0.14231315347876597	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	880	0.14199044791532206	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	878	0.14166774235187815	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	859	0.13860203949916097	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	854	0.1377952755905512	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	843	0.13602039499160964	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	843	0.13602039499160964	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	834	0.13456821995611204	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	771	0.12440299470762876	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	759	0.12246676132696528	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	751	0.12117593907318963	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	747	0.12053052794630179	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	739	0.11923970569252614	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	736	0.11875564734736027	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	730	0.11778753065702853	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	722	0.11649670840325288	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	717	0.1156899444946431	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	712	0.1148831805860333	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	707	0.1140764166774235	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	705	0.1137537111139796	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	701	0.11310829998709176	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	694	0.11197883051503807	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	673	0.10859042209887698	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	664	0.10713824706337938	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	656	0.10584742480960373	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	650	0.10487930811927197	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	638	0.10294307473860849	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	632	0.10197495804827675	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	626	0.10100684135794501	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	622	0.10036143023105719	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	620	0.10003872466761328	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.6135278172195688E-4
7	0.0	0.0	0.0	0.0	1.6135278172195688E-4
8	6.454111268878275E-4	0.0	0.0	0.0	1.6135278172195688E-4
9	6.454111268878275E-4	0.0	0.0	0.0	1.6135278172195688E-4
10	8.067639086097845E-4	0.0	0.0	0.0	1.6135278172195688E-4
11	8.067639086097845E-4	0.0	0.0	0.0	1.6135278172195688E-4
12	8.067639086097845E-4	0.0	0.0	0.0	1.6135278172195688E-4
13	8.067639086097845E-4	0.0	0.0	0.0	1.6135278172195688E-4
14	8.067639086097845E-4	0.0	0.0	1.6135278172195688E-4	1.6135278172195688E-4
15	8.067639086097845E-4	0.0	0.0	9.681166903317413E-4	1.6135278172195688E-4
16	8.067639086097845E-4	0.0	0.0	0.001290822253775655	1.6135278172195688E-4
17	8.067639086097845E-4	0.0	0.0	0.002904350070995224	1.6135278172195688E-4
18	9.681166903317413E-4	0.0	0.0	0.0035497611978830513	1.6135278172195688E-4
19	9.681166903317413E-4	0.0	0.0	0.004840583451658707	1.6135278172195688E-4
20	9.681166903317413E-4	0.0	0.0	0.007583580740931973	1.6135278172195688E-4
21	9.681166903317413E-4	0.0	0.0	0.016135278172195688	1.6135278172195688E-4
22	0.0011294694720536981	0.0	0.0	0.02242803665935201	1.6135278172195688E-4
23	0.0011294694720536981	0.0	0.0	0.027591325674454627	1.6135278172195688E-4
24	0.0011294694720536981	0.0	0.0	0.03388408416161095	3.2270556344391377E-4
25	0.0011294694720536981	0.0	0.0	0.03888602039499161	3.2270556344391377E-4
26	0.0011294694720536981	0.0	0.0	0.04485607331870402	3.2270556344391377E-4
27	0.0011294694720536981	0.0	0.0	0.061636762617787534	3.2270556344391377E-4
28	0.0011294694720536981	0.0	0.0	0.15893248999612752	3.2270556344391377E-4
29	0.0011294694720536981	0.0	0.0	0.33997031108816317	3.2270556344391377E-4
30	0.0011294694720536981	0.0	0.0	0.584097069833484	3.2270556344391377E-4
31	0.001290822253775655	0.0	0.0	1.328740157480315	3.2270556344391377E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1425	0.0	28.042107	1
ATTAGAG	60	1.3357458E-6	24.666666	3
TAATACT	85	1.9717845E-9	23.941175	4
ATACGGC	170	0.0	23.941175	29
TTTTACG	70	1.9199433E-7	23.785713	4
TCTATCG	55	1.900825E-5	23.545454	31
TTTACTT	40	0.001930188	23.125002	23
GTGCTAT	40	0.001930188	23.125002	1
CTATATC	65	2.679535E-6	22.76923	4
ATATCAG	65	2.679535E-6	22.76923	5
TATCTAT	65	2.679535E-6	22.76923	7
GCGGTAA	765	0.0	22.248365	23
TATATCT	70	5.095666E-6	21.142857	5
TATTGCG	70	5.095666E-6	21.142857	11
ACTGTGC	105	9.804353E-10	21.142857	8
ACTATAT	80	6.949467E-7	20.8125	3
CTTTTTA	80	6.949467E-7	20.8125	2
CTAAGTC	80	6.949467E-7	20.8125	34
GTACTAT	80	6.949467E-7	20.8125	1
GCTTTTT	80	6.949467E-7	20.8125	1
>>END_MODULE