FastQCFastQC Report
Fri 10 Feb 2017
ERR1631626.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631626.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451965
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55411.2259798878231722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53181.176639784054075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42860.9483035190778046No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19950.4414058610733132No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17840.3947208301527773No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17500.38719812374852036No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15360.33984932461584416No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG15150.3352029471308619No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13530.2993594636752846No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13490.29847443939243085No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13380.29604062261458297No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13060.2889604283517529No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12580.27834013695750776No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12230.27059617448253737No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11350.25112564025975465No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10320.2283362649762703No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9690.21439713252132353No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9270.20510437755135907No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9220.20399809719779186No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7950.17589857621718497No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7880.1743497837221909No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7760.17169471087362959No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7720.17080968659077583No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7710.1705884305200624No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7700.17036717444934896No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7670.16970340623720861No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7650.16926089409578174No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7390.1635082362572323No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT7270.16085316340867103No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7260.16063190733795757No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7250.16041065126724416No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7220.15974688305510382No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7150.15819809056010972No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7140.1579768344893963No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7080.15664929806511568No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7080.15664929806511568No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA7050.15598552985297534No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC7010.1551005055701216No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6940.1535517130751275No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT6910.15288794486298718No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6480.14337393382230923No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA6400.14160388525660172No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6310.13961258062018075No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC6270.138727556337327No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6190.1369575077716195No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG6180.13673625170090603No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6160.13629373955947915No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.13186861814521036No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA5920.13098359386235658No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5910.13076233779164315No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5830.12899228922593564No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5790.12810726494308186No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG5700.12611596030666092No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5600.12390339959952651No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG5590.12368214352881307No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5440.12036330246811147No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5440.12036330246811147No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5410.11969953425597114No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC5380.11903576604383082No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT5370.11881450997311739No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC5260.11638069319526954No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5220.11549566891241579No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5150.1139468764174217No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG5120.1132831082052814No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5110.11306185213456794No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG5000.1106280353567201No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA4960.10974301107386635No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4960.10974301107386635No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4930.10907924286172602No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4900.1084154746495857No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4820.10664542608387817No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT4770.10553914573031098No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4700.1039903532353169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTC454.0011837E-628.77777934
GGTATCA17950.027.5181061
GTTATCG509.075158E-625.90000211
ATACACA1250.025.16000237
TAAGTCT551.8993489E-523.54545435
GATGGCA400.001929384223.12521
GCTTATT1059.786163E-1021.14285727
CTTATAC6950.020.7625937
TTTACGA450.0038222720.5555575
CTTTTTA450.0038222720.5555572
ATCGTTA555.1364605E-420.18181814
CAGTCGG2750.020.18181810
TATCGTT555.1364605E-420.18181813
GAGGGCA555.1364605E-420.1818189
GGGCAGT2900.019.7758627
TGGGCAG2850.019.4736846
CCGCTTA1052.2502718E-819.3809525
GCAGTCG2900.019.137939
TACTGGT4500.018.9111122
GTACTGG4600.018.9021721
Produced by FastQC (version 0.11.5)