##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1631626.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 451965 Sequences flagged as poor quality 0 Sequence length 43 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64616065403295 34.0 31.0 34.0 31.0 34.0 2 32.79804188377419 34.0 31.0 34.0 31.0 34.0 3 32.85730310975407 34.0 31.0 34.0 31.0 34.0 4 36.2631420574602 37.0 37.0 37.0 35.0 37.0 5 36.21717168364807 37.0 35.0 37.0 35.0 37.0 6 36.306910933368734 37.0 37.0 37.0 35.0 37.0 7 36.29729956965694 37.0 37.0 37.0 35.0 37.0 8 36.28982775214895 37.0 37.0 37.0 35.0 37.0 9 38.110170035290345 39.0 38.0 39.0 37.0 39.0 10 38.058916066509575 39.0 38.0 39.0 35.0 39.0 11 38.13840230991338 39.0 38.0 39.0 37.0 39.0 12 38.06918013562997 39.0 38.0 39.0 37.0 39.0 13 38.122184239930085 39.0 38.0 39.0 37.0 39.0 14 39.45823238525107 40.0 39.0 41.0 37.0 41.0 15 39.479756175810074 41.0 39.0 41.0 37.0 41.0 16 39.41003838792827 40.0 39.0 41.0 37.0 41.0 17 39.42432489241423 40.0 39.0 41.0 37.0 41.0 18 39.49421304747049 41.0 39.0 41.0 37.0 41.0 19 39.49508922151052 41.0 39.0 41.0 37.0 41.0 20 39.51489385240008 41.0 39.0 41.0 37.0 41.0 21 39.466381246335445 41.0 39.0 41.0 37.0 41.0 22 39.41811423450931 41.0 39.0 41.0 36.0 41.0 23 39.34246014625026 41.0 39.0 41.0 36.0 41.0 24 39.33618532408483 41.0 39.0 41.0 36.0 41.0 25 39.2919230471386 40.0 39.0 41.0 36.0 41.0 26 39.18318896374719 40.0 39.0 41.0 35.0 41.0 27 39.015653867002975 40.0 39.0 41.0 35.0 41.0 28 38.99614350668747 40.0 38.0 41.0 35.0 41.0 29 38.940572831967074 40.0 38.0 41.0 35.0 41.0 30 38.81851692055801 40.0 38.0 41.0 35.0 41.0 31 38.63007533769208 40.0 38.0 41.0 35.0 41.0 32 38.49831956014293 40.0 38.0 41.0 35.0 41.0 33 38.365636719657495 40.0 38.0 41.0 35.0 41.0 34 38.28969942362794 40.0 37.0 41.0 35.0 41.0 35 38.149925326076136 40.0 37.0 41.0 35.0 41.0 36 38.03461329970241 40.0 37.0 41.0 34.0 41.0 37 37.92192315776664 40.0 37.0 41.0 34.0 41.0 38 37.82008120097795 40.0 37.0 41.0 34.0 41.0 39 37.6416492427511 40.0 36.0 41.0 33.0 41.0 40 37.24587965882314 40.0 36.0 41.0 33.0 41.0 41 37.18305178498335 40.0 36.0 41.0 33.0 41.0 42 37.0408748465036 40.0 35.0 41.0 33.0 41.0 43 36.51035810295045 39.0 35.0 41.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 1.0 11 0.0 12 1.0 13 1.0 14 0.0 15 4.0 16 1.0 17 4.0 18 4.0 19 8.0 20 20.0 21 47.0 22 69.0 23 169.0 24 278.0 25 504.0 26 824.0 27 1342.0 28 2100.0 29 3178.0 30 4256.0 31 5477.0 32 6795.0 33 8850.0 34 12385.0 35 18212.0 36 26956.0 37 58014.0 38 100947.0 39 201516.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.72860730366289 16.879625634728352 14.16923876848871 25.222528293120046 2 20.282986514442488 21.98134811323886 32.49300277676369 25.242662595554965 3 21.885986746761365 21.629993472945912 30.897967762990497 25.586052017302226 4 14.198444569822884 19.035544787760113 35.370437976391976 31.395572666025025 5 15.67488632969367 33.623400042038654 33.89952761828903 16.802186009978648 6 28.372772227938004 35.45186021041452 17.585211244233516 18.590156317413957 7 26.904074430542185 30.160742535373313 21.458520018143 21.4766630159415 8 26.863805825672344 29.94700917106413 21.49215094089144 21.697034062372087 9 28.863960704921844 12.75873131769053 19.49509364663193 38.8822143307557 10 20.428794265042647 24.08217450466297 28.21612292987289 27.272908300421495 11 36.440874846503604 21.05096633588884 19.346852079253924 23.161306738353634 12 21.004945073180444 26.47218258050955 28.059473631807773 24.46339871450223 13 36.407686435896586 18.158928235593464 22.008341353865895 23.425043974644055 14 22.84734437401126 23.231666168840505 26.929518878674237 26.991470578473997 15 30.2246855398095 24.630889560032305 22.572544334185167 22.57188056597303 16 21.262265883420177 25.266558251192016 27.002975894151092 26.46819997123671 17 24.5156151471906 25.2218645249079 24.289712698992176 25.972807628909315 18 25.11831668381401 20.612436803734802 26.771763300255554 27.497483212195633 19 25.821468476541327 23.40048455079486 28.589381921166463 22.18866505149735 20 28.611507528237805 20.49605611053953 27.22909959842023 23.663336762802427 21 25.08468576106557 22.867036164304757 26.852300509995242 25.195977564634433 22 26.087639529609596 23.6595754096003 26.426825086013295 23.825959974776808 23 25.366123483013066 22.47629794342482 27.57071897160178 24.586859601960327 24 24.925160134081178 22.5269655836182 28.464814753354794 24.083059528945824 25 24.59327602801102 23.41132609825982 28.41658092993927 23.578816943789896 26 24.70722290442844 24.430874072107354 27.51274988107486 23.349153142389344 27 24.06270397044019 22.900003318841062 30.22225172303165 22.815040987687098 28 23.52969809609151 24.772272189218192 26.35093425375859 25.34709546093171 29 24.13593972984634 25.223855829544323 26.919783611562842 23.720420829046496 30 23.91623245162789 22.938280619074487 28.905778102286682 24.239708827010944 31 25.22496210989789 23.66400053101457 27.448806876638677 23.662230482448862 32 22.328941400329672 24.057172568672353 27.769628179173168 25.84425785182481 33 22.424081510736453 23.441638179947564 29.633268062792474 24.501012246523516 34 22.880975296759704 24.032391888752446 28.325865940946755 24.760766873541094 35 22.07936455256491 25.85377186286549 28.857765534941866 23.209098049627734 36 22.922792694124546 24.001637294923277 29.104466053787352 23.971103957164825 37 23.717987012268647 24.41317358645028 27.927162501521135 23.941676899759937 38 22.833405241556314 23.68214352881307 28.428750013828502 25.055701215802106 39 22.86637239609262 22.3771752237452 29.62906419744892 25.12738818271326 40 22.52320423041607 24.058500105096634 29.54410186629496 23.87419379819234 41 20.23032756961269 23.509342537585876 30.575376411890304 25.684953480911133 42 18.696801743497836 24.322901109599197 30.882479838040556 26.097817308862414 43 18.00692531501333 25.715265562598873 29.91736085758853 26.360448264799263 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 16.0 2 27.0 3 106.5 4 186.0 5 186.0 6 315.5 7 445.0 8 505.5 9 566.0 10 892.5 11 1219.0 12 1219.0 13 2543.0 14 3867.0 15 6849.5 16 9832.0 17 9432.5 18 9033.0 19 9033.0 20 9887.0 21 10741.0 22 7653.0 23 4565.0 24 3832.5 25 3100.0 26 3100.0 27 2734.0 28 2368.0 29 2157.5 30 1947.0 31 1886.5 32 1826.0 33 1826.0 34 2409.0 35 2992.0 36 2874.5 37 2757.0 38 4310.0 39 5863.0 40 5863.0 41 9078.5 42 12294.0 43 15747.5 44 19201.0 45 31456.0 46 43711.0 47 43711.0 48 48634.5 49 53558.0 50 59977.0 51 66396.0 52 62649.5 53 58903.0 54 58903.0 55 49528.5 56 40154.0 57 35569.5 58 30985.0 59 27546.0 60 24107.0 61 24107.0 62 21096.0 63 18085.0 64 13534.5 65 8984.0 66 7474.0 67 5964.0 68 5964.0 69 4910.5 70 3857.0 71 3027.0 72 2197.0 73 1606.0 74 1015.0 75 1015.0 76 815.0 77 615.0 78 480.0 79 345.0 80 251.5 81 158.0 82 158.0 83 113.0 84 68.0 85 44.0 86 20.0 87 14.0 88 8.0 89 8.0 90 4.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 451965.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.25687925177219 #Duplication Level Percentage of deduplicated Percentage of total 1 76.71346136059692 20.142560919306852 2 9.113051379140876 4.785605793545961 3 3.7355684774405566 2.9425311134664964 4 2.000803584669485 2.101394325167185 5 1.3461837449821423 1.76732920213473 6 0.976109057093109 1.5377746589192987 7 0.6897253977766017 1.2677025540410545 8 0.5364474914516612 1.126834960636989 9 0.49854984399225694 1.17813267492251 >10 3.416580545885529 18.432636807391773 >50 0.5238139193389849 9.548884147516127 >100 0.3949658009163729 20.500559167388733 >500 0.04126508367783001 7.212078671623424 >1k 0.01179002390795143 5.054823351524144 >5k 0.0016842891297073471 2.4011516524147276 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5541 1.2259798878231722 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 5318 1.176639784054075 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 4286 0.9483035190778046 No Hit GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA 1995 0.4414058610733132 No Hit ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA 1784 0.3947208301527773 No Hit TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA 1750 0.38719812374852036 No Hit CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA 1536 0.33984932461584416 No Hit GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG 1515 0.3352029471308619 No Hit GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA 1353 0.2993594636752846 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1349 0.29847443939243085 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 1338 0.29604062261458297 No Hit TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT 1306 0.2889604283517529 No Hit CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC 1258 0.27834013695750776 No Hit GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC 1223 0.27059617448253737 No Hit GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT 1135 0.25112564025975465 No Hit CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA 1032 0.2283362649762703 No Hit CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA 969 0.21439713252132353 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 927 0.20510437755135907 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 922 0.20399809719779186 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 795 0.17589857621718497 No Hit GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC 788 0.1743497837221909 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 776 0.17169471087362959 No Hit CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA 772 0.17080968659077583 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 771 0.1705884305200624 No Hit GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA 770 0.17036717444934896 No Hit CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA 767 0.16970340623720861 No Hit TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC 765 0.16926089409578174 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 739 0.1635082362572323 No Hit GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT 727 0.16085316340867103 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 726 0.16063190733795757 No Hit GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC 725 0.16041065126724416 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 722 0.15974688305510382 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 715 0.15819809056010972 No Hit CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT 714 0.1579768344893963 No Hit GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC 708 0.15664929806511568 No Hit CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA 708 0.15664929806511568 No Hit AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA 705 0.15598552985297534 No Hit ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC 701 0.1551005055701216 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 694 0.1535517130751275 No Hit CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT 691 0.15288794486298718 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 648 0.14337393382230923 No Hit TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA 640 0.14160388525660172 No Hit GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC 631 0.13961258062018075 No Hit AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC 627 0.138727556337327 No Hit CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT 619 0.1369575077716195 No Hit ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG 618 0.13673625170090603 No Hit GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG 616 0.13629373955947915 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 596 0.13186861814521036 No Hit CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA 592 0.13098359386235658 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 591 0.13076233779164315 No Hit CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG 583 0.12899228922593564 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 579 0.12810726494308186 No Hit GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG 570 0.12611596030666092 No Hit GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA 560 0.12390339959952651 No Hit TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG 559 0.12368214352881307 No Hit CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC 544 0.12036330246811147 No Hit CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC 544 0.12036330246811147 No Hit GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA 541 0.11969953425597114 No Hit GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC 538 0.11903576604383082 No Hit CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT 537 0.11881450997311739 No Hit GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC 526 0.11638069319526954 No Hit CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA 522 0.11549566891241579 No Hit GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT 515 0.1139468764174217 No Hit TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG 512 0.1132831082052814 No Hit GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC 511 0.11306185213456794 No Hit AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG 500 0.1106280353567201 No Hit GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA 496 0.10974301107386635 No Hit CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC 496 0.10974301107386635 No Hit TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA 493 0.10907924286172602 No Hit CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT 490 0.1084154746495857 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 482 0.10664542608387817 No Hit CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT 477 0.10553914573031098 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA 470 0.1039903532353169 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.212560707134402E-4 0.0 4 0.0 0.0 0.0 2.212560707134402E-4 0.0 5 2.212560707134402E-4 0.0 0.0 2.212560707134402E-4 0.0 6 2.212560707134402E-4 0.0 0.0 2.212560707134402E-4 0.0 7 2.212560707134402E-4 0.0 0.0 2.212560707134402E-4 0.0 8 2.212560707134402E-4 0.0 0.0 2.212560707134402E-4 0.0 9 2.212560707134402E-4 0.0 0.0 2.212560707134402E-4 0.0 10 2.212560707134402E-4 0.0 0.0 2.212560707134402E-4 0.0 11 2.212560707134402E-4 0.0 0.0 2.212560707134402E-4 0.0 12 2.212560707134402E-4 0.0 0.0 4.425121414268804E-4 0.0 13 2.212560707134402E-4 0.0 0.0 4.425121414268804E-4 0.0 14 2.212560707134402E-4 0.0 0.0 4.425121414268804E-4 0.0 15 2.212560707134402E-4 0.0 0.0 0.0019913046364209617 0.0 16 4.425121414268804E-4 0.0 0.0 0.002876328919274723 0.0 17 4.425121414268804E-4 0.0 0.0 0.004203865343555364 0.0 18 6.637682121403206E-4 0.0 0.0 0.005310145697122565 0.0 19 6.637682121403206E-4 0.0 0.0 0.007080194262830086 0.0 20 6.637682121403206E-4 0.0 0.0 0.008186474616397288 0.0 21 6.637682121403206E-4 0.0 0.0 0.013275364242806412 0.0 22 6.637682121403206E-4 0.0 0.0 0.02057681457634994 0.0 23 6.637682121403206E-4 0.0 0.0 0.02433816777847842 0.0 24 6.637682121403206E-4 0.0 0.0 0.030090825617027868 0.0 25 6.637682121403206E-4 0.0 0.0 0.03606473952629075 0.0 26 6.637682121403206E-4 0.0 0.0 0.04380870200126116 0.0 27 6.637682121403206E-4 0.0 0.0 0.07987344152755191 0.0 28 6.637682121403206E-4 0.0 0.0 0.2212560707134402 0.0 29 6.637682121403206E-4 0.0 0.0 0.4688416138417798 0.0 30 6.637682121403206E-4 0.0 0.0 0.7823614660427245 0.0 31 6.637682121403206E-4 0.0 0.0 1.6483577268151295 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGTC 45 4.0011837E-6 28.777779 34 GGTATCA 1795 0.0 27.518106 1 GTTATCG 50 9.075158E-6 25.900002 11 ATACACA 125 0.0 25.160002 37 TAAGTCT 55 1.8993489E-5 23.545454 35 GATGGCA 40 0.0019293842 23.125 21 GCTTATT 105 9.786163E-10 21.142857 27 CTTATAC 695 0.0 20.76259 37 TTTACGA 45 0.00382227 20.555557 5 CTTTTTA 45 0.00382227 20.555557 2 ATCGTTA 55 5.1364605E-4 20.181818 14 CAGTCGG 275 0.0 20.181818 10 TATCGTT 55 5.1364605E-4 20.181818 13 GAGGGCA 55 5.1364605E-4 20.181818 9 GGGCAGT 290 0.0 19.775862 7 TGGGCAG 285 0.0 19.473684 6 CCGCTTA 105 2.2502718E-8 19.38095 25 GCAGTCG 290 0.0 19.13793 9 TACTGGT 450 0.0 18.911112 2 GTACTGG 460 0.0 18.902172 1 >>END_MODULE