FastQCFastQC Report
Fri 10 Feb 2017
ERR1631625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16698
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1300.7785363516588814No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1040.622829081327105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT990.592885375494071No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA910.5449754461612168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT850.5090429991615762No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG830.49706551682836264No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA760.4551443286621153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT740.44316684632890163No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC700.41921188166247453No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA660.3952569169960474No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA610.3653132111630135No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT590.35333572882979997No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC510.30542579949694576No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT470.28147083483051866No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA470.28147083483051866No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT430.2575158701640915No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT420.25152712899748475No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG400.2395496466642712No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA400.2395496466642712No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA400.2395496466642712No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC390.23356090549766442No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA390.23356090549766442No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA380.22757216433105765No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC380.22757216433105765No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA380.22757216433105765No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT380.22757216433105765No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG370.22158342316445082No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC360.21559468199784404No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG360.21559468199784404No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC350.20960594083123726No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA320.19163971733141694No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA320.19163971733141694No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA310.18565097616481013No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC310.18565097616481013No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG300.17966223499820336No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC300.17966223499820336No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG290.17367349383159658No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.17367349383159658No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA280.1676847526649898No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC280.1676847526649898No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA270.16169601149838303No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC270.16169601149838303No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA270.16169601149838303No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC260.15570727033177625No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA250.14971852916516948No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC250.14971852916516948No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC250.14971852916516948No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC240.1437297879985627No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG240.1437297879985627No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC240.1437297879985627No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT230.13774104683195593No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA230.13774104683195593No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA230.13774104683195593No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC230.13774104683195593No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA230.13774104683195593No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA230.13774104683195593No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA220.13175230566534915No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG220.13175230566534915No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG220.13175230566534915No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC220.13175230566534915No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC220.13175230566534915No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC220.13175230566534915No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC220.13175230566534915No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC210.12576356449874238No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG210.12576356449874238No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAA210.12576356449874238No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT210.12576356449874238No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG210.12576356449874238No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA200.1197748233321356No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG200.1197748233321356No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG200.1197748233321356No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC200.1197748233321356No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA200.1197748233321356No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG200.1197748233321356No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC190.11378608216552882No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA190.11378608216552882No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC190.11378608216552882No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC190.11378608216552882No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA190.11378608216552882No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG190.11378608216552882No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT190.11378608216552882No Hit
GTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAG180.10779734099892202No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTC180.10779734099892202No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG180.10779734099892202No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC180.10779734099892202No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG180.10779734099892202No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG180.10779734099892202No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT170.10180859983231524No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA170.10180859983231524No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG170.10180859983231524No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT170.10180859983231524No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT170.10180859983231524No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT170.10180859983231524No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC170.10180859983231524No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA170.10180859983231524No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT170.10180859983231524No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGTGTGGACGCCAGTCTG170.10180859983231524No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT170.10180859983231524No Hit
GCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCT170.10180859983231524No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC170.10180859983231524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA307.830595E-637.01
CATATCA250.00534410429.610
GTATCAA451.248355E-424.6666662
TATCAAC852.4719899E-517.4117641
ATCAACG852.4719899E-517.4117642
TCAACGC904.0384242E-516.4444453
CAACGCA904.0384242E-516.4444454
AACGCAG956.4164735E-515.5789485
ACGCAGA1009.943526E-514.86
CGCAGAG1009.943526E-514.87
GCAGAGT1051.5066167E-414.0952388
CAGAGTA1102.2366268E-413.4545459
AGAGTAC1102.2366268E-413.45454510
Produced by FastQC (version 0.11.5)