FastQCFastQC Report
Fri 10 Feb 2017
ERR1631624.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631624.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96701
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.2481877126399934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2380.24611948170132678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2320.23991478888532697No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1610.16649259056266225No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1150.11892327897333017No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1100.11375270162666364No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1020.1054797778719972No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA970.10030920052533066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1600.030.06251
GCCGTAC250.005471793529.59999812
CTTATAC1200.024.66666637
GTATCAA2400.020.0416662
TCGGGAG500.006989525618.49999820
TCTAAGT500.006989525618.4999983
ATCTGTC750.004068373214.836
TCTTATA2005.820766E-1114.79999936
CGCACTT1152.1686641E-514.47826128
GCACTTC1304.343263E-614.23076929
ATCAACG3450.013.9420284
TCAACGC3450.013.9420285
CAACGCA3500.013.7428576
AACGCAG3500.013.7428577
TATCAAC3700.013.53
TACGGGC1102.420092E-413.45454514
ACGCAGA3700.013.08
AGAGTAC3700.013.012
CGCAGAG3700.013.09
CAGAGTA3750.012.82666611
Produced by FastQC (version 0.11.5)