FastQCFastQC Report
Fri 10 Feb 2017
ERR1631622.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631622.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265648
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6270.23602662169487443No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6180.23263867975667046No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5760.21682828404505208No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5500.20704089622357405No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5400.20327651629223634No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4830.1818195506836114No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4800.18069023670421008No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4620.1739143528278022No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4460.16789134493766186No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4140.1558453291573812No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4060.15283382521231104No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3690.13890561946636149No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3550.13363548756248872No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2950.11104920797446245No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2930.1102963319881949No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2800.10540263807745588No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2780.10464976209118833No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2760.1038968861049208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2720.10239113413238572No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2690.1012618201529844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTC200.001839389437.03
CATAAGG200.001839389437.037
TAACTAG200.001839389437.05
GCTCATA200.001839389437.034
TTATTAC303.592097E-430.8333321
AACGATT250.005488831529.622
TCATAAG250.005488831529.636
GGTATCA4050.027.8641971
CTAAACT358.851375E-426.4285724
GCGGTAA2300.025.7391323
AACTCCG2350.024.4042575
TCCGTGC2450.024.1632678
CGCGGTA2500.023.6822
CGTGCCA2500.023.6810
AGCCGCG2600.023.4807719
CGGTAAT2550.023.21568724
CTAACTC2500.022.9399993
TAACTCC2500.022.9399994
CTCCGTG2600.022.769237
GGTAATA2700.022.61111325
Produced by FastQC (version 0.11.5)