FastQCFastQC Report
Fri 10 Feb 2017
ERR1631621.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631621.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349734
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13080.3739985245929764No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12200.3488365443451309No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11520.32939319597179567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.29365174675610606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9400.2687756981019861No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8390.23989660713570884No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8360.2390388123545323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7640.21845173760629508No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7490.21416276370041232No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7460.21330496891923573No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7450.21301903732551022No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7210.20615667907609783No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6770.19357568895217508No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6770.19357568895217508No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6590.18842892026511576No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6580.18814298867139026No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6530.18671333070276266No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6510.18614146751531163No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6470.18499774114040957No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6460.18471180954668406No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6390.18271028839060544No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6140.1755619985474675No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6130.17527606695374198No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6090.17413234057883992No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5890.1684137087043296No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5820.16641218754825096No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5780.1652684611733489No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5550.158692034517662No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5510.1575483081427599No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5430.15526085539295578No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5350.15297340264315165No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5330.15240153945570062No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5250.1501140867058965No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5230.14954222351844546No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5210.14897036033099442No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5100.14582511280001373No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT4980.14239393367530753No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4900.1401064809255034No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4850.13867682295687578No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4760.13610343861334614No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4740.1355315754258951No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4680.13381598586354201No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4610.13181446470746339No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4560.1303848067388358No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4540.12981294355138479No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4460.12752549080158063No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4340.12409431167687442No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4320.1235224484894234No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC4300.12295058530197235No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4190.11980533777099167No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT4070.11637415864628546No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC4030.1152304322713834No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA4020.11494450067765787No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4010.11465856908393235No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4000.11437263749020685No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3980.1138007743027558No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3970.11351484270903028No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3940.11265704792785373No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3800.10865400561569649No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3790.10836807402197098No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT3730.10665248445961786No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3690.1055087580847158No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG3660.10465096330353928No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3660.10465096330353928No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC3660.10465096330353928No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC3640.10407910011608823No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3640.10407910011608823No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT3530.10093385258510754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7950.033.9748421
ATACGGC950.031.15789629
CTAAGTC303.5944046E-430.83333234
CGTATGC250.005491179429.631
CTCGTAT250.005491179429.629
ACCTGCG250.005491179429.616
TGGCAGA250.005491179429.66
TCGTATG250.005491179429.630
AACAGGT250.005491179429.635
TACGGCT1050.028.19047530
TCCTCTC405.928497E-527.757
TCCTTGA358.857009E-426.4285725
ATATGCC701.9149775E-723.78571335
TTAGCTA551.8977547E-523.5454544
TATTAGC551.8977547E-523.5454542
GGAGGGC551.8977547E-523.54545433
ACGGCTG1350.023.29629731
ATGGGAA400.001928515723.12529
TAAGTCT400.001928515723.12535
TCTCCGA400.001928515723.1251
Produced by FastQC (version 0.11.5)