##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631621.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	349734
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5907947182716	34.0	31.0	34.0	31.0	34.0
2	32.75746710357014	34.0	31.0	34.0	31.0	34.0
3	32.827345925760724	34.0	31.0	34.0	31.0	34.0
4	36.24002527635289	37.0	37.0	37.0	35.0	37.0
5	36.17614529899867	37.0	35.0	37.0	35.0	37.0
6	36.250601886004794	37.0	37.0	37.0	35.0	37.0
7	36.24872331543401	37.0	37.0	37.0	35.0	37.0
8	36.23785791487244	37.0	37.0	37.0	35.0	37.0
9	38.069132540730955	39.0	38.0	39.0	37.0	39.0
10	38.00110083663584	39.0	38.0	39.0	35.0	39.0
11	38.07658963669532	39.0	38.0	39.0	37.0	39.0
12	38.003851498567485	39.0	38.0	39.0	35.0	39.0
13	38.071462883219816	39.0	38.0	39.0	35.0	39.0
14	39.40217422383869	40.0	39.0	41.0	37.0	41.0
15	39.4349934521665	40.0	39.0	41.0	37.0	41.0
16	39.36023091835509	40.0	39.0	41.0	36.0	41.0
17	39.356613883694465	40.0	39.0	41.0	36.0	41.0
18	39.39975810187171	40.0	39.0	41.0	37.0	41.0
19	39.403672505389814	40.0	39.0	41.0	37.0	41.0
20	39.41398605797549	40.0	39.0	41.0	37.0	41.0
21	39.37035003745704	40.0	39.0	41.0	36.0	41.0
22	39.33011374358799	40.0	39.0	41.0	36.0	41.0
23	39.2701195765925	40.0	39.0	41.0	36.0	41.0
24	39.2758610829945	40.0	39.0	41.0	36.0	41.0
25	39.218491768029416	40.0	39.0	41.0	36.0	41.0
26	39.148166892552624	40.0	39.0	41.0	36.0	41.0
27	38.998658980825425	40.0	39.0	41.0	35.0	41.0
28	39.00295367336319	40.0	39.0	41.0	35.0	41.0
29	38.976610795633256	40.0	38.0	41.0	35.0	41.0
30	38.91632497841216	40.0	38.0	41.0	35.0	41.0
31	38.81880514905614	40.0	38.0	41.0	35.0	41.0
32	38.7605951952055	40.0	38.0	41.0	35.0	41.0
33	38.695282700566715	40.0	38.0	41.0	35.0	41.0
34	38.65863198888298	40.0	38.0	41.0	35.0	41.0
35	38.569561438121546	40.0	38.0	41.0	35.0	41.0
36	38.494984759846055	40.0	38.0	41.0	35.0	41.0
37	38.44657654102832	40.0	38.0	41.0	35.0	41.0
38	38.40697501529734	40.0	38.0	41.0	34.0	41.0
39	38.306404295836266	40.0	37.0	41.0	34.0	41.0
40	37.9792928339824	40.0	37.0	41.0	34.0	41.0
41	38.027332201044224	40.0	37.0	41.0	34.0	41.0
42	37.98178329816375	40.0	37.0	41.0	34.0	41.0
43	37.50637627454008	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	1.0
19	6.0
20	8.0
21	24.0
22	60.0
23	95.0
24	164.0
25	310.0
26	459.0
27	744.0
28	1193.0
29	1738.0
30	2512.0
31	3481.0
32	4617.0
33	6201.0
34	9271.0
35	13881.0
36	21068.0
37	39680.0
38	83408.0
39	160811.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.93091320832404	16.49939668433724	12.934115642173765	27.635574465164954
2	22.057335003173844	19.654365889504593	32.251368182675975	26.036930924645592
3	21.351083966671812	19.969748437383842	29.971921517496153	28.70724607844819
4	14.903898391348855	17.188205893622012	35.65509787438453	32.252797840644604
5	16.942876586205514	32.74002527635289	32.8689804251231	17.448117712318506
6	30.39252689186639	35.23649402117037	16.810204326716878	17.56077476024636
7	28.769579165880355	28.365843755539927	20.190201696146215	22.674375382433507
8	26.310853391434634	29.78921122910555	21.179810942030226	22.72012443742959
9	28.656636186358774	12.727387099910217	18.167807533725632	40.44816918000538
10	20.53246181383566	22.42418523792368	28.43189395369052	28.611458994550144
11	39.2180914637982	19.90913093951403	18.764546769830787	22.108230826856985
12	22.246907649813856	24.557806790303488	26.677989557778197	26.517296002104455
13	36.669869100516394	17.204218062870638	20.637112777139198	25.48880005947377
14	23.237374690479047	21.33850297654789	24.47088358581093	30.953238747162125
15	30.623845551190332	24.168082028055608	20.179050363990918	25.02902205676314
16	23.613374736228103	24.16922575443051	23.519017310298686	28.698382199042697
17	26.628237460469954	23.789508597963025	21.84260037628598	27.739653565281042
18	27.071717362338234	20.27683896904505	23.50472073061241	29.14672293800431
19	28.399583683599534	22.444772312671915	23.740900227029684	25.414743776698863
20	30.738790051867994	19.712410003030875	23.209639325887675	26.33916061921346
21	27.609268758542203	21.814579080100877	22.244620197064055	28.33153196429286
22	28.13824220693441	23.279692566350427	21.140638313689834	27.441426913025328
23	28.000995041946165	21.410557738166723	22.90769556291353	27.680751656973584
24	27.388529568186108	21.158366072500815	23.631960289820263	27.821144069492814
25	27.3996809003414	22.08106732545306	23.981368697352845	26.53788307685269
26	28.206865789428537	22.802758668016263	22.792465130642146	26.197910411913057
27	26.92331886519469	21.77740797291656	25.212304208341195	26.08696895354755
28	26.293697495811102	23.518445447111233	22.225748711878172	27.962108345199493
29	26.800082348298993	24.210399903926984	22.513681826759765	26.47583592101426
30	26.16188303110364	22.205447568723656	25.267489005930223	26.365180394242483
31	28.01843686916342	22.271497766874255	22.814767794952736	26.895297569009593
32	24.334780147197584	22.26921031412445	23.60336713044771	29.792642408230257
33	24.898065386836855	21.901502284593434	26.197624480319327	27.00280784825038
34	25.132815225285505	21.755391240199696	25.5171072872526	27.594686247262207
35	25.20572778168551	24.293891929294837	25.447625909977294	25.05275437904236
36	25.483081427599263	22.418180674455442	25.95915753115225	26.139580366793048
37	26.04693853042598	22.55171072872526	24.91407755608548	26.48727318476328
38	25.202296602560804	21.129201049940814	25.855078431036162	27.813423916462227
39	24.528069904556034	20.018070876723453	27.56380563513985	27.890053583580666
40	24.306186987825033	21.675044462362823	28.06847489806539	25.950293651746758
41	23.10927733649002	20.361474720787797	28.993749535361165	27.535498407361025
42	20.65026563045057	21.623862707085955	30.434558836144042	27.29131282631943
43	19.878250327391676	22.101654400201294	29.627945810244356	28.392149462162674
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	14.0
4	24.0
5	24.0
6	41.5
7	59.0
8	70.0
9	81.0
10	118.5
11	156.0
12	156.0
13	368.5
14	581.0
15	1177.5
16	1774.0
17	1621.0
18	1468.0
19	1468.0
20	1614.0
21	1760.0
22	1270.0
23	780.0
24	654.5
25	529.0
26	529.0
27	524.5
28	520.0
29	630.0
30	740.0
31	926.0
32	1112.0
33	1112.0
34	1844.0
35	2576.0
36	2767.0
37	2958.0
38	4152.5
39	5347.0
40	5347.0
41	7462.5
42	9578.0
43	12297.5
44	15017.0
45	22748.0
46	30479.0
47	30479.0
48	34563.0
49	38647.0
50	41824.5
51	45002.0
52	44519.0
53	44036.0
54	44036.0
55	39597.0
56	35158.0
57	33048.5
58	30939.0
59	28731.5
60	26524.0
61	26524.0
62	23386.5
63	20249.0
64	15917.5
65	11586.0
66	9891.5
67	8197.0
68	8197.0
69	6770.5
70	5344.0
71	4530.0
72	3716.0
73	2854.0
74	1992.0
75	1992.0
76	1596.0
77	1200.0
78	931.0
79	662.0
80	517.0
81	372.0
82	372.0
83	310.5
84	249.0
85	235.0
86	221.0
87	146.5
88	72.0
89	72.0
90	45.0
91	18.0
92	11.5
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	349734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.50158347539756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.03364518013576	22.13611346745137
2	10.009702819377184	5.906041665795565
3	3.9198241985273956	3.469230624052179
4	2.2340854253505946	2.636362306685985
5	1.5217386472256875	2.244684986443359
6	1.0044854738414675	1.7780347233814993
7	0.8147694001868371	1.6825891230968102
8	0.5970979775342959	1.4092268661775271
9	0.4662955603926245	1.2380811659217263
>10	3.5805907306511573	21.198494545784694
>50	0.4553121508795016	9.455134204668903
>100	0.3265067397754321	19.38305457665333
>500	0.03195172988628082	6.076406890582284
>1k	0.003993966235785102	1.3865448533047708
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1308	0.3739985245929764	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1220	0.3488365443451309	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1152	0.32939319597179567	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1027	0.29365174675610606	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	940	0.2687756981019861	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	839	0.23989660713570884	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	836	0.2390388123545323	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	764	0.21845173760629508	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	749	0.21416276370041232	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	746	0.21330496891923573	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	745	0.21301903732551022	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	721	0.20615667907609783	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	677	0.19357568895217508	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	677	0.19357568895217508	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	659	0.18842892026511576	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	658	0.18814298867139026	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	653	0.18671333070276266	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	651	0.18614146751531163	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	647	0.18499774114040957	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	646	0.18471180954668406	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	639	0.18271028839060544	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	614	0.1755619985474675	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	613	0.17527606695374198	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	609	0.17413234057883992	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	589	0.1684137087043296	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	582	0.16641218754825096	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	578	0.1652684611733489	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	555	0.158692034517662	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	551	0.1575483081427599	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	543	0.15526085539295578	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	535	0.15297340264315165	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	533	0.15240153945570062	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	525	0.1501140867058965	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	523	0.14954222351844546	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	521	0.14897036033099442	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	510	0.14582511280001373	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	498	0.14239393367530753	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	490	0.1401064809255034	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	485	0.13867682295687578	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	476	0.13610343861334614	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	474	0.1355315754258951	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	468	0.13381598586354201	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	461	0.13181446470746339	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	456	0.1303848067388358	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	454	0.12981294355138479	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	446	0.12752549080158063	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	434	0.12409431167687442	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	432	0.1235224484894234	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	430	0.12295058530197235	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	419	0.11980533777099167	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	407	0.11637415864628546	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	403	0.1152304322713834	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	402	0.11494450067765787	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	401	0.11465856908393235	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	400	0.11437263749020685	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	398	0.1138007743027558	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	397	0.11351484270903028	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	394	0.11265704792785373	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	380	0.10865400561569649	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	379	0.10836807402197098	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT	373	0.10665248445961786	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	369	0.1055087580847158	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	366	0.10465096330353928	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	366	0.10465096330353928	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	366	0.10465096330353928	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	364	0.10407910011608823	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	364	0.10407910011608823	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT	353	0.10093385258510754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.859315937255171E-4	0.0
4	0.0	0.0	0.0	2.859315937255171E-4	0.0
5	0.0	0.0	0.0	2.859315937255171E-4	0.0
6	0.0	0.0	0.0	2.859315937255171E-4	0.0
7	0.0	0.0	0.0	2.859315937255171E-4	0.0
8	0.0	0.0	0.0	2.859315937255171E-4	0.0
9	0.0	0.0	0.0	2.859315937255171E-4	0.0
10	0.0	0.0	0.0	5.718631874510342E-4	0.0
11	0.0	0.0	0.0	5.718631874510342E-4	0.0
12	0.0	0.0	0.0	5.718631874510342E-4	0.0
13	0.0	0.0	0.0	5.718631874510342E-4	0.0
14	0.0	0.0	0.0	5.718631874510342E-4	0.0
15	0.0	0.0	0.0	5.718631874510342E-4	0.0
16	0.0	0.0	0.0	0.0020015211560786197	0.0
17	0.0	0.0	0.0	0.0034311791247062053	0.0
18	2.859315937255171E-4	0.0	0.0	0.0037171107184317226	0.0
19	2.859315937255171E-4	0.0	0.0	0.004860837093333791	0.0
20	2.859315937255171E-4	0.0	0.0	0.007434221436863445	0.0
21	2.859315937255171E-4	0.0	0.0	0.012580990123922752	0.0
22	2.859315937255171E-4	0.0	0.0	0.018299621998433095	0.0
23	2.859315937255171E-4	0.0	0.0	0.02087300634196275	0.0
24	2.859315937255171E-4	0.0	0.0	0.024590117060394472	0.0
25	2.859315937255171E-4	0.0	0.0	0.02659163821647309	0.0
26	2.859315937255171E-4	0.0	0.0	0.03259620168470895	0.0
27	2.859315937255171E-4	0.0	0.0	0.05890190830745652	0.0
28	2.859315937255171E-4	0.0	0.0	0.1855696043278606	0.0
29	2.859315937255171E-4	0.0	0.0	0.4040213419341557	0.0
30	2.859315937255171E-4	0.0	0.0	0.7068228996894783	0.0
31	2.859315937255171E-4	0.0	0.0	1.4385218480330766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	795	0.0	33.974842	1
ATACGGC	95	0.0	31.157896	29
CTAAGTC	30	3.5944046E-4	30.833332	34
CGTATGC	25	0.0054911794	29.6	31
CTCGTAT	25	0.0054911794	29.6	29
ACCTGCG	25	0.0054911794	29.6	16
TGGCAGA	25	0.0054911794	29.6	6
TCGTATG	25	0.0054911794	29.6	30
AACAGGT	25	0.0054911794	29.6	35
TACGGCT	105	0.0	28.190475	30
TCCTCTC	40	5.928497E-5	27.75	7
TCCTTGA	35	8.857009E-4	26.42857	25
ATATGCC	70	1.9149775E-7	23.785713	35
TTAGCTA	55	1.8977547E-5	23.545454	4
TATTAGC	55	1.8977547E-5	23.545454	2
GGAGGGC	55	1.8977547E-5	23.545454	33
ACGGCTG	135	0.0	23.296297	31
ATGGGAA	40	0.0019285157	23.125	29
TAAGTCT	40	0.0019285157	23.125	35
TCTCCGA	40	0.0019285157	23.125	1
>>END_MODULE