FastQCFastQC Report
Fri 10 Feb 2017
ERR1631620.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631620.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13633
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA660.4841194161226436No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA640.4694491307855938No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA590.43277341744296927No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC510.37409227609477No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG440.3227462774150957No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA430.31541113474657084No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA420.3080759920780459No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT400.29340570674099614No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA360.2640651360668965No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA310.22738942272427198No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA310.22738942272427198No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA300.22005428005574706No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT290.2127191373872222No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT290.2127191373872222No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA280.20538399471869728No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC260.19071370938164747No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA230.16870828137607274No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT230.16870828137607274No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT230.16870828137607274No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT210.15403799603902296No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA210.15403799603902296No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC210.15403799603902296No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT210.15403799603902296No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA200.14670285337049807No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA200.14670285337049807No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG200.14670285337049807No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC200.14670285337049807No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT190.13936771070197315No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC190.13936771070197315No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA190.13936771070197315No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG180.13203256803344826No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG170.12469742536492334No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC160.11736228269639845No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA160.11736228269639845No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC160.11736228269639845No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC160.11736228269639845No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC150.11002714002787353No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT150.11002714002787353No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC150.11002714002787353No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC150.11002714002787353No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC150.11002714002787353No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA140.10269199735934864No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG140.10269199735934864No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC140.10269199735934864No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG140.10269199735934864No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG140.10269199735934864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAAAT200.001778731837.027
AGGCAAA200.001778731837.026
CTCACTG200.001778731837.09
TCTCCAG250.00530985829.5999981
CTCTTAT603.390341E-521.58333437
TCTCTTA803.08579E-416.187536
GTCTCTT1204.5705092E-412.33333437
TGTCTCT1450.002232493110.20689736
CTGTCTC1600.0050473869.2535
Produced by FastQC (version 0.11.5)