FastQCFastQC Report
Fri 10 Feb 2017
ERR1631619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745641
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20710.2777476023984733No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA19560.2623246307539419No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA18180.2438170647805043No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC17650.23670908654432896No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16300.21860385896161827No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16070.215519264632712No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG15910.2133734598821685No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14860.19929161620672683No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14510.19459766831491293No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT14390.19298831475200534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14340.19231775076746047No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13480.1807840502332892No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13100.17568776395074842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11660.156375521195857No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10870.14578061024004849No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10570.14175722633277946No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA10220.13706327844096555No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA9390.1259319162975212No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT8650.11600756932625754No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8490.11386176457571405No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7950.10661967354262977No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT7850.10527854557354009No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7770.10420564319826833No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7700.10326685361990556No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG7490.10045048488481724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.030.3731351
GACGAAT250.005495119429.621
ATACGGC905.456968E-1226.72222129
TAGACTC400.001930554523.1255
AACGATT400.001930554523.12522
GTGTAAG400.001930554523.1251
TACGGCT1107.4578566E-1121.86363630
TGCGGGT5750.021.23478321
TCTTACC705.097958E-621.1428572
CCATCCG705.097958E-621.1428579
GTAACGT5700.021.0964926
ATACACA1500.020.96666737
AGAGCTA450.003824566720.5555555
TATACAC2800.020.48214137
GTTTATA1101.7516868E-920.1818181
GATTATC1101.7516868E-920.18181824
TTCTGCG6150.020.1544718
GTTCTAG851.2441087E-619.5882341
GACCGTT951.6741433E-719.4736847
CGGGTAA6300.019.3809523
Produced by FastQC (version 0.11.5)