FastQCFastQC Report
Fri 10 Feb 2017
ERR1631617.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631617.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242436
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6860.28296127637809565No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6770.27924895642561337No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5960.2458380768532726No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5500.22686399709614083No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5480.2260390371067003No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5270.21737695721757494No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5180.21366463726509266No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5160.21283967727565214No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4950.20417759738652674No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4700.19386559751852034No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4370.1802537576927519No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4340.17901631770859114No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4150.17117919780890628No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3230.13323103829464272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.1295187183421604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3140.1295187183421604No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3130.12910623834744014No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3030.12498143840023758No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3030.12498143840023758No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3020.12456895840551732No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2900.11961919846887425No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2860.11796927848999324No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2860.11796927848999324No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2810.11590687851639196No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2700.11136959857446914No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2680.11054463858502864No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2570.10600735864310581No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2540.10476991865894505No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2520.10394495866950453No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2470.10188255869590325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2450.10105759870646273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGCC200.001839072737.07
TAAACCA250.00548789629.65
TCGTAAG250.00548789629.627
GGTATCA4900.027.5612241
ATACACA556.2275467E-726.9090937
CTAAGTC358.849135E-426.4285734
TCCCATG358.849135E-426.428572
ATGCAAG358.849135E-426.4285711
GCGGTAA2000.023.12500223
ATCTAGA400.001926817823.1251
CCCGATT400.001926817823.12524
CCGCTTA502.693587E-422.225
CCGCGGT2200.021.86363621
GCAGTCG1201.0913936E-1121.5833349
AGCCGCG2250.021.37777519
ACTGCAT806.913597E-720.81258
CTTATTG1251.8189894E-1120.7228
CAGTCGG1251.8189894E-1120.7210
GTATTAG450.003817235120.5555551
CTGTTTG450.003817235120.5555559
Produced by FastQC (version 0.11.5)