##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631617.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	242436
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.593286475605936	34.0	31.0	34.0	31.0	34.0
2	32.76119470705671	34.0	31.0	34.0	31.0	34.0
3	32.84435067399231	34.0	31.0	34.0	31.0	34.0
4	36.261384447854276	37.0	37.0	37.0	35.0	37.0
5	36.18746803280041	37.0	35.0	37.0	35.0	37.0
6	36.24774373442888	37.0	37.0	37.0	35.0	37.0
7	36.244415020871486	37.0	37.0	37.0	35.0	37.0
8	36.2258987939085	37.0	37.0	37.0	35.0	37.0
9	38.06008183603095	39.0	38.0	39.0	35.0	39.0
10	37.9901169793265	39.0	38.0	39.0	35.0	39.0
11	38.0623752248016	39.0	38.0	39.0	35.0	39.0
12	37.989160025738755	39.0	38.0	39.0	35.0	39.0
13	38.05544968569024	39.0	38.0	39.0	35.0	39.0
14	39.41622943787226	40.0	39.0	41.0	37.0	41.0
15	39.42889669851012	40.0	39.0	41.0	37.0	41.0
16	39.37481644640235	40.0	39.0	41.0	36.0	41.0
17	39.346483195565014	40.0	39.0	41.0	36.0	41.0
18	39.38637413585441	40.0	39.0	41.0	37.0	41.0
19	39.39881453249517	40.0	39.0	41.0	37.0	41.0
20	39.39977973568282	40.0	39.0	41.0	37.0	41.0
21	39.36985843686581	40.0	39.0	41.0	36.0	41.0
22	39.33752000527974	40.0	39.0	41.0	36.0	41.0
23	39.273300994901746	40.0	39.0	41.0	36.0	41.0
24	39.29451484103021	40.0	39.0	41.0	36.0	41.0
25	39.241527660908446	40.0	39.0	41.0	36.0	41.0
26	39.18164381527496	40.0	39.0	41.0	36.0	41.0
27	39.0671641175403	40.0	39.0	41.0	35.0	41.0
28	39.05297893052187	40.0	39.0	41.0	35.0	41.0
29	39.02685244765629	40.0	39.0	41.0	35.0	41.0
30	38.9876091009586	40.0	38.0	41.0	35.0	41.0
31	38.904073652427854	40.0	38.0	41.0	35.0	41.0
32	38.8668514577043	40.0	38.0	41.0	35.0	41.0
33	38.802591199326834	40.0	38.0	41.0	35.0	41.0
34	38.781224735600325	40.0	38.0	41.0	35.0	41.0
35	38.72187711395997	40.0	38.0	41.0	35.0	41.0
36	38.68045174809022	40.0	38.0	41.0	35.0	41.0
37	38.63132125591909	40.0	38.0	41.0	35.0	41.0
38	38.58505749971126	40.0	38.0	41.0	35.0	41.0
39	38.50927255028131	40.0	38.0	41.0	35.0	41.0
40	38.202643171806166	40.0	38.0	41.0	34.0	41.0
41	38.288847365902754	40.0	37.0	41.0	34.0	41.0
42	38.26830998696563	40.0	37.0	41.0	34.0	41.0
43	37.78963107789272	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	1.0
19	5.0
20	10.0
21	25.0
22	37.0
23	63.0
24	113.0
25	181.0
26	324.0
27	458.0
28	791.0
29	1167.0
30	1630.0
31	2235.0
32	2962.0
33	4227.0
34	6132.0
35	9060.0
36	14085.0
37	25643.0
38	58613.0
39	114670.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.37695721757495	16.52807338844066	13.041792473065057	28.053176920919338
2	20.775379894075137	19.580837829365276	32.65191638205547	26.99186589450412
3	20.76795525417017	19.657971588377965	29.778580738834165	29.795492418617698
4	14.975911168308336	16.554059628108035	34.9861406721774	33.48388853140622
5	16.422890989786996	32.97488821792143	34.107970763417974	16.4942500288736
6	32.609843422594004	34.569535877509935	16.129617713540892	16.691002986355162
7	29.468808262799257	28.177333399330134	20.454882938177498	21.898975399693114
8	26.925044135359432	30.476909369895562	20.916035572274744	21.68201092247026
9	28.43265851606197	12.731607517035423	17.99278984969229	40.842944117210315
10	19.290864393076937	23.573231698262635	29.529030342028413	27.606873566632018
11	38.62215182563646	19.795327426619806	18.761240079856126	22.82128066788761
12	22.29701859459816	24.42005312742332	26.702717418205218	26.580210859773302
13	35.06327443119009	17.9115312907324	20.76176805424937	26.263426223828144
14	23.75637281591843	20.89087429259681	23.571994258278473	31.78075863320629
15	29.820653698295637	24.21092577010015	20.309277500041247	25.65914303156297
16	24.543384645844675	24.746737283241764	22.993284825685954	27.716593245227607
17	26.454816941378347	24.159365770760118	21.898562919698396	27.487254368163143
18	26.64167037898662	21.45720932534772	23.54765793858998	28.35346235707568
19	27.85312412348001	22.540381791483114	23.72956161626161	25.876932468775266
20	30.108564734610372	20.802603573726675	22.992872345691236	26.09595934597172
21	27.292151330660463	22.391064033394382	22.03220643798776	28.2845781979574
22	28.288290517909882	23.204062102988004	21.624676203204142	26.882971175897968
23	27.837862363675363	22.378277153558052	22.643501790163175	27.14035869260341
24	27.135408932666767	21.851127720305566	23.478361299476973	27.53510204755069
25	27.45013116863832	22.119652196868454	23.608704977808575	26.82151165668465
26	27.26987741094557	23.29934498176838	22.76023362866901	26.67054397861704
27	26.629708459139735	22.059430117639295	24.66382880430299	26.647032618917983
28	25.885594548664393	23.185088023230875	22.47067267237539	28.458644755729345
29	26.819861736705768	23.926314573743173	22.740434588922437	26.513389100628622
30	26.544737580227356	22.215760035638272	24.17462753056477	27.064874853569602
31	27.389496609414444	22.885627547063965	23.13394050388556	26.59093533963603
32	25.316372155950432	22.525532511673184	23.416076820274217	28.742018512102163
33	25.30482271609827	22.112640036958208	25.30399775610883	27.278539490834696
34	25.87033278885974	22.16626243627184	24.43407744724381	27.529327327624607
35	25.105594878648386	23.75884769588675	25.152617598046493	25.98293982741837
36	25.7024534310086	22.496246432048046	25.500750713590392	26.300549423352965
37	26.284875183553595	22.349816033922355	25.07795871900213	26.28735006352192
38	25.30894751604547	21.48773284495702	25.89755646851128	27.30576317048623
39	24.82139616228613	20.617812536092	27.13045917273012	27.43033212889175
40	24.3565312082364	21.71500932204788	27.958719002128397	25.96974046758732
41	22.79281954825191	20.74609381445	28.93299674965764	27.52808988764045
42	21.31572868715867	21.380075566335034	29.784767938754968	27.519427807751324
43	20.34557573957663	22.06932963751258	29.511706182250165	28.07338844066063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	6.0
4	10.0
5	10.0
6	17.5
7	25.0
8	26.5
9	28.0
10	40.0
11	52.0
12	52.0
13	123.0
14	194.0
15	371.5
16	549.0
17	531.5
18	514.0
19	514.0
20	612.5
21	711.0
22	583.5
23	456.0
24	521.5
25	587.0
26	587.0
27	660.5
28	734.0
29	995.5
30	1257.0
31	1521.5
32	1786.0
33	1786.0
34	2336.5
35	2887.0
36	3253.5
37	3620.0
38	4623.5
39	5627.0
40	5627.0
41	6797.0
42	7967.0
43	9410.0
44	10853.0
45	15247.5
46	19642.0
47	19642.0
48	21866.0
49	24090.0
50	26823.5
51	29557.0
52	29862.5
53	30168.0
54	30168.0
55	26812.0
56	23456.0
57	21889.0
58	20322.0
59	18887.5
60	17453.0
61	17453.0
62	15688.0
63	13923.0
64	11332.0
65	8741.0
66	7377.5
67	6014.0
68	6014.0
69	5121.0
70	4228.0
71	3525.5
72	2823.0
73	2227.5
74	1632.0
75	1632.0
76	1317.5
77	1003.0
78	808.0
79	613.0
80	504.5
81	396.0
82	396.0
83	325.5
84	255.0
85	200.5
86	146.0
87	102.0
88	58.0
89	58.0
90	46.0
91	34.0
92	25.0
93	16.0
94	10.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	242436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.80264268422789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.47881133394496	38.602263358824715
2	7.843843032963085	7.342251654858833
3	2.8338382016978705	3.978933503369411
4	1.51874235161088	2.8432462244739205
5	0.975597639234672	2.2830273856338312
6	0.7092590161119777	1.9917117780993556
7	0.5042436067750783	1.6519953347590215
8	0.4007897584384952	1.500641588455592
9	0.31028509014766703	1.306994598398223
>10	2.0874546577808473	19.113788803930362
>50	0.21381738031950523	6.95897316546438
>100	0.11536193542819816	10.276715170910846
>500	0.007955995546772288	2.1494574328215172
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	686	0.28296127637809565	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	677	0.27924895642561337	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	596	0.2458380768532726	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	550	0.22686399709614083	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	548	0.2260390371067003	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	527	0.21737695721757494	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	518	0.21366463726509266	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	516	0.21283967727565214	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	495	0.20417759738652674	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	470	0.19386559751852034	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	437	0.1802537576927519	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	434	0.17901631770859114	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	415	0.17117919780890628	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	323	0.13323103829464272	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	314	0.1295187183421604	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	314	0.1295187183421604	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	313	0.12910623834744014	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	303	0.12498143840023758	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	303	0.12498143840023758	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	302	0.12456895840551732	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	290	0.11961919846887425	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	286	0.11796927848999324	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	286	0.11796927848999324	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	281	0.11590687851639196	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	270	0.11136959857446914	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	268	0.11054463858502864	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	257	0.10600735864310581	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	254	0.10476991865894505	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	252	0.10394495866950453	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	247	0.10188255869590325	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	245	0.10105759870646273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.124799947202561E-4	0.0	0.0	0.0	0.0
6	4.124799947202561E-4	0.0	0.0	0.0	0.0
7	4.124799947202561E-4	0.0	0.0	0.0	0.0
8	4.124799947202561E-4	0.0	0.0	0.0	0.0
9	4.124799947202561E-4	0.0	0.0	0.0	0.0
10	8.249599894405122E-4	0.0	0.0	0.0	0.0
11	8.249599894405122E-4	0.0	0.0	0.0	0.0
12	8.249599894405122E-4	0.0	0.0	0.0	0.0
13	8.249599894405122E-4	0.0	0.0	0.0	0.0
14	8.249599894405122E-4	0.0	0.0	0.0	0.0
15	8.249599894405122E-4	0.0	0.0	0.0	0.0
16	8.249599894405122E-4	0.0	0.0	4.124799947202561E-4	0.0
17	8.249599894405122E-4	0.0	0.0	4.124799947202561E-4	0.0
18	8.249599894405122E-4	0.0	0.0	8.249599894405122E-4	0.0
19	8.249599894405122E-4	0.0	0.0	0.0020623999736012802	0.0
20	8.249599894405122E-4	0.0	0.0	0.0028873599630417925	0.0
21	8.249599894405122E-4	0.0	0.0	0.005362239931363329	0.0
22	0.0012374399841607682	0.0	0.0	0.012374399841607682	0.0
23	0.0012374399841607682	0.0	0.0	0.014849279809929219	0.0
24	0.0012374399841607682	0.0	0.0	0.017736639772971012	0.0
25	0.0012374399841607682	0.0	0.0	0.02021151974129255	4.124799947202561E-4
26	0.0012374399841607682	0.0	0.0	0.02309887970433434	4.124799947202561E-4
27	0.0012374399841607682	0.0	0.0	0.04248543945618637	4.124799947202561E-4
28	0.0012374399841607682	0.0	0.0	0.1398307182101668	4.124799947202561E-4
29	0.0012374399841607682	0.0	0.0	0.3180220759293174	4.124799947202561E-4
30	0.0012374399841607682	0.0	0.0	0.5452985530201785	4.124799947202561E-4
31	0.0012374399841607682	0.0	0.0	1.1673183850583246	4.124799947202561E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTGCC	20	0.0018390727	37.0	7
TAAACCA	25	0.005487896	29.6	5
TCGTAAG	25	0.005487896	29.6	27
GGTATCA	490	0.0	27.561224	1
ATACACA	55	6.2275467E-7	26.90909	37
CTAAGTC	35	8.849135E-4	26.42857	34
TCCCATG	35	8.849135E-4	26.42857	2
ATGCAAG	35	8.849135E-4	26.42857	11
GCGGTAA	200	0.0	23.125002	23
ATCTAGA	40	0.0019268178	23.125	1
CCCGATT	40	0.0019268178	23.125	24
CCGCTTA	50	2.693587E-4	22.2	25
CCGCGGT	220	0.0	21.863636	21
GCAGTCG	120	1.0913936E-11	21.583334	9
AGCCGCG	225	0.0	21.377775	19
ACTGCAT	80	6.913597E-7	20.8125	8
CTTATTG	125	1.8189894E-11	20.72	28
CAGTCGG	125	1.8189894E-11	20.72	10
GTATTAG	45	0.0038172351	20.555555	1
CTGTTTG	45	0.0038172351	20.555555	9
>>END_MODULE