##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631616.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4586
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.717618839947665	31.0	31.0	34.0	30.0	34.0
2	31.911905800261668	31.0	31.0	34.0	30.0	34.0
3	32.032926297426954	33.0	31.0	34.0	30.0	34.0
4	35.694068905364155	37.0	35.0	37.0	33.0	37.0
5	35.35041430440471	37.0	35.0	37.0	33.0	37.0
6	35.363933711295246	37.0	35.0	37.0	33.0	37.0
7	35.31748800697776	37.0	35.0	37.0	33.0	37.0
8	35.28630614914959	37.0	35.0	37.0	32.0	37.0
9	37.11491495856956	39.0	37.0	39.0	33.0	39.0
10	36.83602267771479	38.0	37.0	39.0	33.0	39.0
11	36.98124727431313	39.0	37.0	39.0	33.0	39.0
12	36.822067160924554	38.0	37.0	39.0	33.0	39.0
13	36.95224596598343	39.0	37.0	39.0	33.0	39.0
14	38.02376798953336	39.0	37.0	40.0	33.0	41.0
15	38.018534670737026	39.0	37.0	40.0	33.0	41.0
16	37.916266899258616	39.0	37.0	40.0	33.0	41.0
17	37.980593109463584	39.0	37.0	40.0	33.0	41.0
18	37.98255560401221	40.0	37.0	40.0	33.0	41.0
19	37.96467509812473	39.0	37.0	40.0	33.0	41.0
20	37.90143916266899	40.0	37.0	40.0	33.0	41.0
21	37.93327518534671	40.0	37.0	40.0	33.0	41.0
22	37.798081116441345	39.0	37.0	40.0	33.0	41.0
23	37.75926733536851	39.0	37.0	40.0	33.0	41.0
24	37.79873528129089	39.0	37.0	40.0	33.0	41.0
25	37.698866114260795	39.0	37.0	40.0	33.0	41.0
26	37.6404273877017	39.0	37.0	40.0	33.0	41.0
27	37.35979066724815	39.0	36.0	40.0	32.0	41.0
28	37.329481029219366	39.0	36.0	40.0	31.0	41.0
29	37.360008722197996	39.0	37.0	40.0	32.0	41.0
30	37.21565634539904	39.0	36.0	40.0	31.0	41.0
31	37.222852158744004	39.0	36.0	40.0	31.0	41.0
32	37.12974269515918	39.0	36.0	40.0	31.0	41.0
33	37.00981247274313	39.0	36.0	40.0	31.0	41.0
34	36.989969472307024	39.0	36.0	40.0	31.0	41.0
35	36.909725250763195	39.0	36.0	40.0	31.0	41.0
36	36.816179677278676	38.0	35.0	40.0	31.0	41.0
37	36.79001308329699	38.0	35.0	40.0	31.0	41.0
38	36.80462276493677	38.0	35.0	40.0	31.0	41.0
39	36.692324465765374	38.0	35.0	40.0	31.0	41.0
40	36.205625817706064	38.0	35.0	40.0	30.0	41.0
41	36.33122546881814	38.0	35.0	40.0	30.0	41.0
42	36.279982555604015	38.0	35.0	40.0	30.0	41.0
43	35.74225904928042	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	3.0
23	2.0
24	8.0
25	12.0
26	22.0
27	23.0
28	29.0
29	58.0
30	59.0
31	83.0
32	163.0
33	183.0
34	272.0
35	363.0
36	598.0
37	935.0
38	1223.0
39	547.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.36807675534235	16.964675098124726	13.017880505887483	27.649367640645444
2	21.82730047972089	20.497165285651985	30.61491495856956	27.06061927605757
3	21.631051024858262	20.80244221543829	29.197557784561713	28.368948975141734
4	15.285651984300044	17.640645442651547	35.23767989533362	31.83602267771478
5	17.030091583078935	32.4465765372874	33.994766681203664	16.528565198430005
6	31.50894025294374	34.605320540776276	16.44134321849106	17.444395987788923
7	29.393807239424337	27.518534670737026	20.170082860880942	22.9175752289577
8	27.474923680767553	29.372001744439597	20.344526820758833	22.808547754034016
9	28.129088530309637	12.320104666375926	17.68425643262102	41.86655037069342
10	19.385085041430443	23.724378543392934	29.590056694286957	27.300479720889665
11	38.704753597906674	19.254252071522025	19.34147405146097	22.699520279110335
12	22.307021369385087	23.63715656345399	28.02006105538596	26.035761011774966
13	36.938508504143044	17.29175752289577	20.51897078063672	25.250763192324467
14	24.02965547317924	20.30091583078936	22.96118621892717	32.708242477104235
15	29.917139119058	23.37549062363716	19.16703009158308	27.540340165721762
16	24.683820322721324	24.20409943305713	22.808547754034016	28.30353249018753
17	26.40645442651548	24.313126907980813	21.54382904491932	27.73658962058439
18	26.232010466637597	21.609245529873526	23.048408198866113	29.110335804622768
19	28.82686436982119	23.092019188835586	22.939380723942435	25.14173571740079
20	30.353249018752727	19.930222416048846	23.026602703881377	26.689925861317054
21	27.03881378107283	20.8678587003925	22.28521587440035	29.80811164413432
22	29.677278674225903	23.00479720889664	19.821194941125164	27.49672917575229
23	27.3222852158744	22.78674225904928	21.58744003488879	28.30353249018753
24	27.23506323593546	21.76188399476668	22.830353249018753	28.17269952027911
25	28.259921500218056	22.04535542956825	23.113824683820322	26.580898386393372
26	28.434365460095947	22.830353249018753	21.783689489751417	26.951591801133883
27	27.671173135630177	21.26035761011775	23.98604448320977	27.0824247710423
28	26.10117749672918	22.65590928914086	22.808547754034016	28.434365460095947
29	27.518534670737026	24.55298735281291	21.107719145224596	26.820758831225465
30	27.126035761011774	22.263410379415614	24.40034888791976	26.210204971652857
31	28.82686436982119	22.65590928914086	22.001744439598777	26.515481901439163
32	25.2725686873092	22.72132577409507	23.658962058438725	28.347143480157
33	26.188399476668124	22.481465329262974	24.247710423026604	27.0824247710423
34	25.2725686873092	21.958133449629308	23.222852158744004	29.546445704317488
35	25.599651112080245	24.182293938072394	25.18534670737026	25.032708242477103
36	26.188399476668124	22.808547754034016	25.11993022241605	25.883122546881815
37	26.64631487134758	22.35063235935456	24.70562581770606	26.297426951591802
38	24.662014827736588	21.456607064980375	25.926733536851287	27.954644570431746
39	25.730484081988664	20.497165285651985	26.64631487134758	27.126035761011774
40	24.26951591801134	21.958133449629308	27.017008286088096	26.75534234627126
41	22.525076319232447	20.606192760575663	28.783253379851725	28.085477540340165
42	22.263410379415614	21.456607064980375	29.088530309638028	27.191452245965987
43	21.456607064980375	22.764936764064544	28.19450501526385	27.58395115569123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	1.5
11	2.0
12	2.0
13	2.5
14	3.0
15	7.0
16	11.0
17	9.0
18	7.0
19	7.0
20	12.5
21	18.0
22	14.0
23	10.0
24	7.5
25	5.0
26	5.0
27	6.5
28	8.0
29	16.0
30	24.0
31	27.0
32	30.0
33	30.0
34	37.5
35	45.0
36	53.5
37	62.0
38	71.5
39	81.0
40	81.0
41	110.0
42	139.0
43	167.5
44	196.0
45	271.5
46	347.0
47	347.0
48	386.0
49	425.0
50	486.5
51	548.0
52	539.5
53	531.0
54	531.0
55	494.0
56	457.0
57	457.5
58	458.0
59	434.0
60	410.0
61	410.0
62	346.0
63	282.0
64	222.0
65	162.0
66	140.0
67	118.0
68	118.0
69	97.5
70	77.0
71	58.5
72	40.0
73	39.0
74	38.0
75	38.0
76	30.5
77	23.0
78	17.0
79	11.0
80	9.5
81	8.0
82	8.0
83	5.5
84	3.0
85	2.5
86	2.0
87	1.0
88	0.0
89	0.0
90	1.0
91	2.0
92	1.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4586.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.01351940689054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.49120042027845	75.11993022241606
2	5.38481744155503	8.940252943741823
3	1.8124507486209613	4.513737461840384
4	1.103230890464933	3.663323157435674
5	0.2626740215392698	1.0902747492368077
6	0.1313370107696349	0.6541648495420846
7	0.31520882584712373	1.831661578717837
8	0.15760441292356187	1.0466637592673353
9	0.15760441292356187	1.1774967291757523
>10	0.18387181507748881	1.962494548626254
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	17	0.3706934147405146	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	15	0.3270824247710423	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	13	0.28347143480157	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	12	0.26166593981683384	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	12	0.26166593981683384	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	11	0.2398604448320977	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	10	0.2180549498473615	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	9	0.19624945486262538	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	9	0.19624945486262538	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	9	0.19624945486262538	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	9	0.19624945486262538	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.19624945486262538	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	9	0.19624945486262538	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	8	0.17444395987788924	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.17444395987788924	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	8	0.17444395987788924	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	8	0.17444395987788924	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	8	0.17444395987788924	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	8	0.17444395987788924	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	7	0.15263846489315308	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	7	0.15263846489315308	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	7	0.15263846489315308	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	7	0.15263846489315308	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	7	0.15263846489315308	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	7	0.15263846489315308	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	7	0.15263846489315308	TruSeq Adapter, Index 10 (95% over 21bp)
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	7	0.15263846489315308	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	7	0.15263846489315308	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	7	0.15263846489315308	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	7	0.15263846489315308	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	7	0.15263846489315308	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	6	0.13083296990841692	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	6	0.13083296990841692	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	6	0.13083296990841692	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	6	0.13083296990841692	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	6	0.13083296990841692	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	5	0.10902747492368076	No Hit
CCGCTACACCATTGCCGCCCTGCTGAGCCCCTACTCCTATTCC	5	0.10902747492368076	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	5	0.10902747492368076	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	5	0.10902747492368076	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT	5	0.10902747492368076	No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC	5	0.10902747492368076	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	5	0.10902747492368076	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	5	0.10902747492368076	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	5	0.10902747492368076	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	5	0.10902747492368076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.021805494984736152	0.0
10	0.0	0.0	0.0	0.021805494984736152	0.0
11	0.0	0.0	0.0	0.021805494984736152	0.0
12	0.0	0.0	0.0	0.021805494984736152	0.0
13	0.0	0.0	0.0	0.021805494984736152	0.0
14	0.0	0.0	0.0	0.021805494984736152	0.0
15	0.0	0.0	0.0	0.043610989969472304	0.0
16	0.0	0.0	0.0	0.043610989969472304	0.0
17	0.0	0.0	0.0	0.043610989969472304	0.0
18	0.0	0.0	0.0	0.043610989969472304	0.0
19	0.0	0.0	0.0	0.043610989969472304	0.0
20	0.0	0.0	0.0	0.043610989969472304	0.0
21	0.0	0.0	0.0	0.043610989969472304	0.0
22	0.0	0.0	0.0	0.043610989969472304	0.0
23	0.0	0.0	0.0	0.043610989969472304	0.0
24	0.0	0.0	0.0	0.043610989969472304	0.0
25	0.0	0.0	0.0	0.043610989969472304	0.0
26	0.0	0.0	0.0	0.043610989969472304	0.0
27	0.0	0.0	0.0	0.06541648495420846	0.0
28	0.0	0.0	0.0	0.17444395987788922	0.0
29	0.0	0.0	0.0	0.34888791975577843	0.0
30	0.0	0.0	0.0	0.45791539467945924	0.0
31	0.0	0.0	0.0	1.0248582642825992	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE