FastQCFastQC Report
Fri 10 Feb 2017
ERR1631615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117970
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2570.2178519962702382No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2460.20852759176061708No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2240.18987878274137493No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1950.16529626176146478No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1950.16529626176146478No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1840.15597185725184368No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1830.1551241841146054No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1770.15003814529117573No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1710.14495210646774603No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1620.1373230482326015No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1480.12545562431126558No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1460.123760278036789No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1380.11697889293888275No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1310.1110451809782148No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1260.10680681529202339No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1210.10256844960583199No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1210.10256844960583199No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1210.10256844960583199No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1200.10172077646859372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1050.031.7142851
TTATACA556.1820356E-726.9090937
CTCTGAG358.8220957E-426.428572
ACGTCAA451.3134035E-424.66666629
GCCAACC400.001920986223.1258
TAATACG652.6491143E-622.7692327
GCGGTAA652.6491143E-622.7692323
GGTAACG502.6822343E-422.225
TGCGGGT502.6822343E-422.221
CTGGTAC502.6822343E-422.23
TGGTTCA502.6822343E-422.25
GTCAATG502.6822343E-422.231
CGTCAAT502.6822343E-422.230
GTAACGT502.6822343E-422.226
TACTGGT603.6899266E-521.5833322
AGCCGCG705.0381786E-621.14285719
GTATCAA1600.020.81252
CAATGAG450.003805792220.55555533
AGTCGGT450.003805792220.55555511
TCTTATA1900.020.44736937