##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631615.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117970
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.612392981266424	34.0	31.0	34.0	31.0	34.0
2	32.77657879121811	34.0	31.0	34.0	31.0	34.0
3	32.86407561244384	34.0	31.0	34.0	31.0	34.0
4	36.28213952699839	37.0	37.0	37.0	35.0	37.0
5	36.20367042468424	37.0	35.0	37.0	35.0	37.0
6	36.26598287700263	37.0	37.0	37.0	35.0	37.0
7	36.25025854030686	37.0	37.0	37.0	35.0	37.0
8	36.22799016699161	37.0	37.0	37.0	35.0	37.0
9	38.0753835720946	39.0	38.0	39.0	37.0	39.0
10	37.98725947274731	39.0	38.0	39.0	35.0	39.0
11	38.074256166822074	39.0	38.0	39.0	37.0	39.0
12	37.996134610494195	39.0	38.0	39.0	35.0	39.0
13	38.0560142409087	39.0	38.0	39.0	35.0	39.0
14	39.46148173264389	40.0	39.0	41.0	37.0	41.0
15	39.46199881325761	41.0	39.0	41.0	37.0	41.0
16	39.41466474527422	40.0	39.0	41.0	37.0	41.0
17	39.3764262100534	40.0	39.0	41.0	36.0	41.0
18	39.39310841739425	40.0	39.0	41.0	37.0	41.0
19	39.405382724421465	41.0	39.0	41.0	37.0	41.0
20	39.404628295329324	41.0	39.0	41.0	37.0	41.0
21	39.366076121047726	40.0	39.0	41.0	36.0	41.0
22	39.34995337797745	40.0	39.0	41.0	36.0	41.0
23	39.269432906671184	40.0	39.0	41.0	36.0	41.0
24	39.2999067559549	40.0	39.0	41.0	36.0	41.0
25	39.23809443078749	40.0	39.0	41.0	36.0	41.0
26	39.17738408069848	40.0	39.0	41.0	36.0	41.0
27	39.05941341018903	40.0	39.0	41.0	35.0	41.0
28	39.04915656522845	40.0	39.0	41.0	35.0	41.0
29	39.02662541324065	40.0	39.0	41.0	35.0	41.0
30	38.98781046028651	40.0	39.0	41.0	35.0	41.0
31	38.90951089259981	40.0	38.0	41.0	35.0	41.0
32	38.88075781978469	40.0	38.0	41.0	35.0	41.0
33	38.80907857929982	40.0	38.0	41.0	35.0	41.0
34	38.78529287106892	40.0	38.0	41.0	35.0	41.0
35	38.71144358735272	40.0	38.0	41.0	35.0	41.0
36	38.680834110367044	40.0	38.0	41.0	35.0	41.0
37	38.62725269136221	40.0	38.0	41.0	35.0	41.0
38	38.5751377468848	40.0	38.0	41.0	35.0	41.0
39	38.51634313808595	40.0	38.0	41.0	34.0	41.0
40	38.21534288378401	40.0	38.0	41.0	34.0	41.0
41	38.29817750275494	40.0	38.0	41.0	34.0	41.0
42	38.28847164533356	40.0	37.0	41.0	34.0	41.0
43	37.81928456387217	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0
19	4.0
20	7.0
21	14.0
22	13.0
23	39.0
24	63.0
25	100.0
26	152.0
27	251.0
28	393.0
29	552.0
30	840.0
31	1063.0
32	1439.0
33	2021.0
34	3017.0
35	4477.0
36	6868.0
37	11942.0
38	27571.0
39	57141.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.455709078579304	16.46520301771637	12.762566754259558	28.316521149444775
2	20.851911502924473	19.62532847334068	32.29210816309231	27.230651860642535
3	20.622192082732898	19.677884207849452	29.45155548020683	30.248368229210815
4	15.53276256675426	15.845553954395186	33.76197338306349	34.859710095787065
5	16.589810968890394	33.42968551326608	33.5627701958125	16.417733322031026
6	34.44011189285411	33.86793252521827	15.347969822836314	16.343985759091296
7	30.501822497245062	27.864711367296767	19.91608035941341	21.717385776044758
8	27.264558786132064	31.29524455370009	20.293294905484448	21.146901754683395
9	28.308892091209632	12.913452572687973	17.2874459608375	41.4902093752649
10	18.78528439433754	24.10612867678223	29.75332711706366	27.355259811816563
11	39.429515978638634	19.465118250402643	18.66321946257523	22.44214630838349
12	22.906671187590067	23.973891667373064	26.326184623209294	26.793252521827583
13	34.66050690853607	17.46121895397135	21.254556243112653	26.62371789437993
14	24.135797236585574	19.949139611765705	22.706620327201833	33.20844282444689
15	29.27947783334746	24.35873527167924	19.96439772823599	26.397389166737305
16	25.859116724590997	24.27481563109265	22.107315419174366	27.758752225141986
17	27.0127998643723	24.162922776977197	21.574976688988727	27.24930066966178
18	26.48385182673561	21.695346274476563	23.494956344833433	28.325845553954395
19	28.232601508858185	22.405696363482242	23.17029753327117	26.191404594388402
20	29.693142324319744	20.990082224294312	22.728659828770027	26.58811562261592
21	27.38069000593371	22.249724506230397	21.70212765957447	28.667457828261423
22	28.399593116894124	22.857506145630243	21.2113249131135	27.531575824362125
23	27.757904552004746	22.515893871323218	22.327710434856318	27.398491141815718
24	28.07662965160634	21.86403322878698	22.510807832499786	27.548529287106895
25	28.191913198270747	22.064084089175214	22.93803509366788	26.805967618886157
26	28.004577434941087	22.50063575485293	22.464185809951683	27.0306010002543
27	26.973806900059337	21.82843095702297	23.75180130541663	27.44596083750106
28	26.318555564974144	23.036365177587523	22.12511655505637	28.519962702381964
29	27.311180808680174	23.277952021700433	22.17851996270238	27.232347206917012
30	26.59320166143935	22.698991268966687	23.361871662287022	27.345935407306943
31	27.87996948376706	22.650673900144106	22.81681783504281	26.652538781046033
32	25.256421124014576	22.4557090785793	23.23387301856404	29.053996778842077
33	25.771806391455453	22.158175807408664	24.289226074425702	27.78079172671018
34	26.381283377129776	22.212426888191914	23.848436043061795	27.55785369161651
35	25.738747139103165	23.622107315419175	24.212087818937018	26.42705772654065
36	26.073578028312284	22.661693650928203	24.564719844028144	26.700008476731373
37	26.906840722217513	21.847079766042214	24.37907942697296	26.867000084767312
38	25.509027718911586	21.569890650165295	25.643807747732474	27.277273883190638
39	25.092820208527595	20.624735102144612	26.477070441637707	27.80537424769009
40	24.6944138340256	21.536831397813003	27.523946766126983	26.244808002034414
41	23.010934983470374	20.702721030770537	28.330083919640586	27.956260066118503
42	21.954734254471475	21.06382978723404	29.073493260998557	27.907942697295923
43	21.196914469780452	21.452064084089177	29.447317114520644	27.90370433160973
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	1.5
4	2.0
5	2.0
6	3.0
7	4.0
8	6.5
9	9.0
10	13.0
11	17.0
12	17.0
13	33.5
14	50.0
15	99.0
16	148.0
17	150.5
18	153.0
19	153.0
20	184.0
21	215.0
22	200.5
23	186.0
24	194.0
25	202.0
26	202.0
27	272.5
28	343.0
29	498.0
30	653.0
31	778.0
32	903.0
33	903.0
34	1131.0
35	1359.0
36	1557.5
37	1756.0
38	2364.5
39	2973.0
40	2973.0
41	3605.0
42	4237.0
43	4713.5
44	5190.0
45	6871.0
46	8552.0
47	8552.0
48	9624.0
49	10696.0
50	12067.5
51	13439.0
52	14028.5
53	14618.0
54	14618.0
55	13102.0
56	11586.0
57	10833.0
58	10080.0
59	9310.5
60	8541.0
61	8541.0
62	7758.5
63	6976.0
64	5817.0
65	4658.0
66	4012.0
67	3366.0
68	3366.0
69	2896.5
70	2427.0
71	2089.0
72	1751.0
73	1420.5
74	1090.0
75	1090.0
76	889.5
77	689.0
78	568.5
79	448.0
80	368.5
81	289.0
82	289.0
83	227.0
84	165.0
85	127.5
86	90.0
87	74.0
88	58.0
89	58.0
90	44.0
91	30.0
92	21.0
93	12.0
94	8.0
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	117970.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.97685852335339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.07689543657922	50.76544884292616
2	6.1775062881782254	7.286598287700263
3	2.3672296083363276	4.188352971094346
4	1.2461372619475386	2.9397304399423585
5	0.7703916636722961	2.2717640077985926
6	0.5519223859144807	1.9530389081969992
7	0.4254401724757456	1.756378740357718
8	0.2932087675170679	1.3834025599728743
9	0.2601509162773985	1.3808595405611597
>10	1.6888250089831118	18.457234890226328
>50	0.11067193675889328	4.666440620496736
>100	0.03162055335968379	2.9507501907264557
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	257	0.2178519962702382	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	246	0.20852759176061708	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	224	0.18987878274137493	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	195	0.16529626176146478	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	195	0.16529626176146478	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	184	0.15597185725184368	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	183	0.1551241841146054	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	177	0.15003814529117573	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	171	0.14495210646774603	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	162	0.1373230482326015	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	148	0.12545562431126558	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	146	0.123760278036789	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	138	0.11697889293888275	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	131	0.1110451809782148	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	126	0.10680681529202339	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	121	0.10256844960583199	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	121	0.10256844960583199	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	121	0.10256844960583199	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	120	0.10172077646859372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	8.476731372382809E-4	0.0	0.0	0.0	0.0
9	8.476731372382809E-4	0.0	0.0	0.0	0.0
10	8.476731372382809E-4	0.0	0.0	0.0	0.0
11	8.476731372382809E-4	0.0	0.0	0.0	0.0
12	8.476731372382809E-4	0.0	0.0	0.0	0.0
13	8.476731372382809E-4	0.0	0.0	0.0	0.0
14	8.476731372382809E-4	0.0	0.0	0.0	0.0
15	8.476731372382809E-4	0.0	0.0	0.0	0.0
16	8.476731372382809E-4	0.0	0.0	8.476731372382809E-4	0.0
17	8.476731372382809E-4	0.0	0.0	0.0016953462744765618	0.0
18	8.476731372382809E-4	0.0	0.0	0.0016953462744765618	0.0
19	8.476731372382809E-4	0.0	0.0	0.0016953462744765618	0.0
20	8.476731372382809E-4	0.0	0.0	0.0016953462744765618	0.0
21	8.476731372382809E-4	0.0	0.0	0.004238365686191405	0.0
22	8.476731372382809E-4	0.0	0.0	0.0059337119606679666	0.0
23	8.476731372382809E-4	0.0	0.0	0.0059337119606679666	0.0
24	8.476731372382809E-4	0.0	0.0	0.006781385097906247	0.0
25	8.476731372382809E-4	0.0	0.0	0.006781385097906247	0.0
26	8.476731372382809E-4	0.0	0.0	0.00932440450962109	0.0
27	8.476731372382809E-4	0.0	0.0	0.020344155293718743	0.0
28	8.476731372382809E-4	0.0	0.0	0.10765448842926167	0.0
29	8.476731372382809E-4	0.0	0.0	0.24158684411291007	0.0
30	8.476731372382809E-4	0.0	0.0	0.4501144358735272	0.0
31	8.476731372382809E-4	0.0	0.0	0.9070102568449606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	105	0.0	31.714285	1
TTATACA	55	6.1820356E-7	26.90909	37
CTCTGAG	35	8.8220957E-4	26.42857	2
ACGTCAA	45	1.3134035E-4	24.666666	29
GCCAACC	40	0.0019209862	23.125	8
TAATACG	65	2.6491143E-6	22.76923	27
GCGGTAA	65	2.6491143E-6	22.76923	23
GGTAACG	50	2.6822343E-4	22.2	25
TGCGGGT	50	2.6822343E-4	22.2	21
CTGGTAC	50	2.6822343E-4	22.2	3
TGGTTCA	50	2.6822343E-4	22.2	5
GTCAATG	50	2.6822343E-4	22.2	31
CGTCAAT	50	2.6822343E-4	22.2	30
GTAACGT	50	2.6822343E-4	22.2	26
TACTGGT	60	3.6899266E-5	21.583332	2
AGCCGCG	70	5.0381786E-6	21.142857	19
GTATCAA	160	0.0	20.8125	2
CAATGAG	45	0.0038057922	20.555555	33
AGTCGGT	45	0.0038057922	20.555555	11
TCTTATA	190	0.0	20.447369	37
>>END_MODULE