FastQCFastQC Report
Fri 10 Feb 2017
ERR1631614.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631614.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133351
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2660.19947356975200786No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2360.17697655060704456No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC2240.16797774294905926No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2040.1529797301857504No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1990.14923022699492317No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1970.14773042571859227No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1760.13198251231711797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.12223380402096723No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT1630.12223380402096723No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1560.11698449955380912No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1550.11623459891564368No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1530.1147347976393128No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1520.11398489700114735No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1480.11098529444848557No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1400.10498608934316203No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1370.10273638742866571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAATA200.001836107937.035
ATACGGC308.256964E-637.029
GGTATCA1950.032.256411
TACGGCT352.3726254E-531.71428530
GAATAAA303.582589E-430.83333437
ACGTGGG303.582589E-430.8333347
TATACAC551.8299033E-830.27272837
TAACGTG250.00547915329.5999985
CCTAACG250.00547915329.5999983
TTAACCC250.00547915329.59999834
ACGGCTG405.9014365E-527.75000231
TAATACG905.456968E-1226.72222327
GGTAATA1200.024.66666825
GCTTAAA451.3146622E-424.6666682
GCGGTAA1002.0008883E-1124.0523
CGTGCCA1200.023.12500210
AGAGACC400.001922296623.12500215
AGCCGCG1053.8198777E-1122.9047619
AATACGG1053.8198777E-1122.9047628
TTATACA753.689729E-722.236
Produced by FastQC (version 0.11.5)