FastQCFastQC Report
Fri 10 Feb 2017
ERR1631613.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631613.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279965
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC8760.31289625488900397No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT6650.23752969121140144No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6210.22181344096583502No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5490.19609594056399907No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5360.19145250299144537No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5310.18966656546354008No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4960.17716500276820316No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4910.1753790652402979No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG4420.15787687746682622No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4100.14644687728823247No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG4030.1439465647491651No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT3900.13930312717661136No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT3860.13787437715428716No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3840.13716000214312504No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3600.12858750200917973No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3600.12858750200917973No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3440.12287250191988285No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3410.12180093940313969No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3270.11680031432500491No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC3220.11501437679709964No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT3140.11215687675245119No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.1050131266408301No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT2940.1050131266408301No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG2850.10179843909060061No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2820.10072687657385745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT303.5925873E-430.83333430
GGTATCA4050.030.6049391
ATCTAAT250.005489332629.5999981
ATCTAAG358.852574E-426.42857232
AGAAGAG2400.025.43750220
ATGCTAG701.9121399E-723.78571515
CATCAGA2600.023.48077216
ACCATGT400.001927559723.1250028
AGTATTA400.001927559723.12500215
CCTCACG400.001927559723.1250022
ATACGGC400.001927559723.12500229
GCATCAC400.001927559723.1250027
CTAATCC400.001927559723.1250023
GATGCTA652.6708258E-622.76923414
GACAGGC2450.022.653067
CCAGGAC2950.022.5762713
AGGCCAT2700.021.92592625
TAATACT855.1455572E-821.7647064
ATACTGG1201.0913936E-1121.5833346
ATCAAGC2750.021.52727130
Produced by FastQC (version 0.11.5)