##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631613.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	279965
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30182344221599	33.0	31.0	34.0	31.0	34.0
2	32.48995410140553	34.0	31.0	34.0	31.0	34.0
3	32.57065347454146	34.0	31.0	34.0	31.0	34.0
4	36.086789420106086	37.0	35.0	37.0	35.0	37.0
5	35.96983194327862	37.0	35.0	37.0	35.0	37.0
6	36.0114371439287	37.0	35.0	37.0	35.0	37.0
7	36.01135856267748	37.0	35.0	37.0	35.0	37.0
8	35.97882949654421	37.0	35.0	37.0	35.0	37.0
9	37.782633543478646	39.0	37.0	39.0	35.0	39.0
10	37.67953851374279	39.0	37.0	39.0	35.0	39.0
11	37.762445305663206	39.0	37.0	39.0	35.0	39.0
12	37.684306966942295	39.0	37.0	39.0	35.0	39.0
13	37.74058185844659	39.0	37.0	39.0	35.0	39.0
14	39.06582608540353	40.0	38.0	41.0	36.0	41.0
15	39.047741681995966	40.0	38.0	41.0	36.0	41.0
16	39.007722393870665	40.0	38.0	41.0	36.0	41.0
17	38.9560445055632	40.0	38.0	41.0	36.0	41.0
18	38.94874716482418	40.0	38.0	41.0	36.0	41.0
19	38.97334666833354	40.0	38.0	41.0	36.0	41.0
20	38.94196060221813	40.0	38.0	41.0	35.0	41.0
21	38.91796117371814	40.0	38.0	41.0	35.0	41.0
22	38.880010001250156	40.0	38.0	41.0	35.0	41.0
23	38.82618541603415	40.0	38.0	41.0	35.0	41.0
24	38.82217777222153	40.0	38.0	41.0	35.0	41.0
25	38.774000321468755	40.0	38.0	41.0	35.0	41.0
26	38.67605236368832	40.0	38.0	41.0	35.0	41.0
27	38.57478970585609	40.0	38.0	41.0	35.0	41.0
28	38.55648741807011	40.0	38.0	41.0	35.0	41.0
29	38.52552640508635	40.0	38.0	41.0	34.0	41.0
30	38.47236976050578	40.0	38.0	41.0	34.0	41.0
31	38.41487685960745	40.0	38.0	41.0	34.0	41.0
32	38.371364277677564	40.0	38.0	41.0	34.0	41.0
33	38.29058632329041	40.0	38.0	41.0	34.0	41.0
34	38.24596646009323	40.0	38.0	41.0	34.0	41.0
35	38.14472523351133	40.0	38.0	41.0	33.0	41.0
36	38.11955065811798	40.0	37.0	41.0	33.0	41.0
37	38.09315807333059	40.0	37.0	41.0	33.0	41.0
38	38.0613505259586	40.0	37.0	41.0	33.0	41.0
39	37.99602807493794	40.0	37.0	41.0	33.0	41.0
40	37.659711035307986	40.0	37.0	41.0	33.0	41.0
41	37.73257014269641	40.0	37.0	41.0	33.0	41.0
42	37.713489186148266	40.0	37.0	41.0	33.0	41.0
43	37.19798546246852	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	12.0
20	17.0
21	23.0
22	51.0
23	116.0
24	231.0
25	349.0
26	549.0
27	849.0
28	1240.0
29	1874.0
30	2668.0
31	3543.0
32	4832.0
33	6420.0
34	9379.0
35	13792.0
36	21186.0
37	35938.0
38	79120.0
39	97770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.51936849249014	15.842694622542103	12.066865501044774	28.57107138392299
2	20.86653688853964	18.48338185130284	33.123426142553534	27.526655117603987
3	21.36802814637544	19.642455306913366	28.48641794510028	30.50309860161092
4	15.530512742664262	15.146178986659049	33.43096458485883	35.89234368581787
5	16.933902452092227	33.20772239387067	32.98197989034344	16.876395263693674
6	35.60837961888093	33.33202364581287	14.881145857517906	16.178450877788293
7	31.556801743075024	27.42057042844641	19.39635311556802	21.626274712910543
8	28.36854606825853	31.085314235708033	19.28205311378208	21.264086582251355
9	29.24544139446002	12.489775507652743	16.554569321165147	41.710213776722085
10	19.612451556444555	23.283624738806637	28.995767328058864	28.10815637668994
11	39.631382494240356	19.218473737788653	18.360152161877377	22.78999160609362
12	24.756308824317326	22.967513796367403	25.65499258835926	26.621184790956015
13	34.32821959887843	17.1153537049274	20.72366188630722	27.83276480988695
14	24.09729787652028	19.06595467290554	23.206472237601126	33.63027521297305
15	29.869805154215705	23.252906613326665	20.10894218920222	26.76834604325541
16	26.011822906434734	23.57258943082171	21.111924704873825	29.303662957869737
17	26.923722608183166	23.79618880931545	21.397317521833088	27.882771060668297
18	26.913364170521316	20.92368688943261	23.01359098458736	29.149357955458715
19	28.556069508688587	21.617345025271014	22.373153787080525	27.453431678959873
20	29.117925454967587	20.726162198846286	22.717839729966247	27.438072616219884
21	29.310449520475775	21.19586376868537	21.15871626810494	28.334970442733916
22	29.67228046362938	22.558534102477097	20.464700944760953	27.30448448913257
23	28.84824888825389	21.96417409319022	21.257657207150892	27.929919811404996
24	28.89254013894594	21.31016377047131	21.604486275070098	28.192809815512653
25	28.199953565624273	21.32052220813316	22.941439108459985	27.53808511778258
26	28.94933295233333	22.043469719429215	22.226706909792295	26.78049041844516
27	28.761809511903273	21.149429392959835	22.588537852945905	27.50022324219099
28	26.851213544550212	21.837015341203365	22.16848534638258	29.14328576786384
29	28.080652938760203	22.299215973425248	22.490311288911112	27.129819798903434
30	27.11196042362438	21.173003768328183	23.564016930687764	28.151018877359668
31	27.573089493329523	22.18098690907792	22.477095351204614	27.76882824638794
32	25.7164288393192	21.92666940510421	22.95965567124462	29.397246084331968
33	26.96872823388638	22.079188469987322	23.58366224349472	27.368421052631582
34	27.278766988730734	22.046327219473863	23.290054113907097	27.384851677888307
35	26.73227010519172	23.12253317378958	24.032289750504525	26.11290697051417
36	27.25769292590145	21.875591591806117	23.26826567606665	27.598449806225776
37	26.385798224778096	21.982390655974854	23.868340685442824	27.763470433804226
38	26.827281981676283	20.94869001482328	24.75416569928384	27.469862304216598
39	25.1545728930402	20.65722501026914	25.701784151590378	28.48641794510028
40	25.467826335434786	20.750093761720215	27.087314485739288	26.69476541710571
41	23.60045005625703	20.032146875502292	27.94813637418963	28.419266694051043
42	22.17884378404443	21.14478595538728	28.37890450592038	28.297465754647906
43	21.82594252853035	20.31539656742807	28.946475452288677	28.912185451752897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	4.0
2	2.0
3	6.5
4	11.0
5	11.0
6	15.5
7	20.0
8	23.5
9	27.0
10	31.5
11	36.0
12	36.0
13	100.5
14	165.0
15	327.5
16	490.0
17	446.5
18	403.0
19	403.0
20	467.0
21	531.0
22	435.0
23	339.0
24	355.0
25	371.0
26	371.0
27	454.0
28	537.0
29	753.0
30	969.0
31	1309.5
32	1650.0
33	1650.0
34	2422.0
35	3194.0
36	3735.0
37	4276.0
38	5476.5
39	6677.0
40	6677.0
41	7897.5
42	9118.0
43	10896.0
44	12674.0
45	15802.5
46	18931.0
47	18931.0
48	20830.0
49	22729.0
50	25788.0
51	28847.0
52	30027.0
53	31207.0
54	31207.0
55	29600.0
56	27993.0
57	25839.5
58	23686.0
59	21913.0
60	20140.0
61	20140.0
62	19582.5
63	19025.0
64	15270.0
65	11515.0
66	9996.5
67	8478.0
68	8478.0
69	7284.5
70	6091.0
71	5804.0
72	5517.0
73	6142.0
74	6767.0
75	6767.0
76	5751.5
77	4736.0
78	3189.5
79	1643.0
80	1080.0
81	517.0
82	517.0
83	417.5
84	318.0
85	239.5
86	161.0
87	123.5
88	86.0
89	86.0
90	72.0
91	58.0
92	37.5
93	17.0
94	11.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	279965.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.08416098299269
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.26695803748515	32.17433667589675
2	8.482169525947366	6.800012975262182
3	3.178926972167634	3.822738615166349
4	1.7200704709651045	2.7579032664102923
5	1.1179436071618705	2.2405915759691983
6	0.7660920316964855	1.8424893795785922
7	0.5443691296896055	1.5274405880054844
8	0.44102747582784446	1.4142573071204991
9	0.34169062528797484	1.2326743827380355
>10	2.5745825150422776	20.21238230060794
>50	0.35261440543629785	9.90711569739313
>100	0.20759552320052466	14.563854241648647
>500	0.0059596800918810915	1.5042029942029194
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	876	0.31289625488900397	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	665	0.23752969121140144	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	621	0.22181344096583502	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	549	0.19609594056399907	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	536	0.19145250299144537	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	531	0.18966656546354008	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	496	0.17716500276820316	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	491	0.1753790652402979	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	442	0.15787687746682622	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	410	0.14644687728823247	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	403	0.1439465647491651	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	390	0.13930312717661136	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	386	0.13787437715428716	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	384	0.13716000214312504	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	360	0.12858750200917973	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	360	0.12858750200917973	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	344	0.12287250191988285	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	341	0.12180093940313969	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	327	0.11680031432500491	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	322	0.11501437679709964	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	314	0.11215687675245119	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	294	0.1050131266408301	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	294	0.1050131266408301	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	285	0.10179843909060061	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	282	0.10072687657385745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.571875055810548E-4	0.0	0.0	0.0	0.0
9	3.571875055810548E-4	0.0	0.0	3.571875055810548E-4	0.0
10	3.571875055810548E-4	0.0	0.0	3.571875055810548E-4	0.0
11	3.571875055810548E-4	0.0	0.0	3.571875055810548E-4	0.0
12	3.571875055810548E-4	0.0	0.0	3.571875055810548E-4	0.0
13	3.571875055810548E-4	0.0	0.0	3.571875055810548E-4	0.0
14	3.571875055810548E-4	0.0	0.0	3.571875055810548E-4	0.0
15	3.571875055810548E-4	0.0	0.0	7.143750111621096E-4	0.0
16	7.143750111621096E-4	0.0	0.0	0.0014287500223242192	0.0
17	7.143750111621096E-4	0.0	0.0	0.002143125033486329	0.0
18	7.143750111621096E-4	0.0	0.0	0.0025003125390673834	0.0
19	7.143750111621096E-4	0.0	0.0	0.003214687550229493	0.0
20	7.143750111621096E-4	0.0	0.0	0.005000625078134767	0.0
21	7.143750111621096E-4	0.0	0.0	0.00892968763952637	0.0
22	7.143750111621096E-4	0.0	0.0	0.011787187684174808	0.0
23	7.143750111621096E-4	0.0	0.0	0.013930312717661136	0.0
24	7.143750111621096E-4	0.0	0.0	0.01857375029021485	0.0
25	7.143750111621096E-4	0.0	0.0	0.020002500312539067	0.0
26	7.143750111621096E-4	0.0	0.0	0.02464593788509278	0.0
27	0.0010715625167431644	0.0	0.0	0.04429125069205079	0.0
28	0.0010715625167431644	0.0	0.0	0.13073062704266605	0.0
29	0.0010715625167431644	0.0	0.0	0.2707481292304395	0.0
30	0.0010715625167431644	0.0	0.0	0.45755719464933114	0.0
31	0.0010715625167431644	0.0	0.0	1.0483453288803957	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCT	30	3.5925873E-4	30.833334	30
GGTATCA	405	0.0	30.604939	1
ATCTAAT	25	0.0054893326	29.599998	1
ATCTAAG	35	8.852574E-4	26.428572	32
AGAAGAG	240	0.0	25.437502	20
ATGCTAG	70	1.9121399E-7	23.785715	15
CATCAGA	260	0.0	23.480772	16
ACCATGT	40	0.0019275597	23.125002	8
AGTATTA	40	0.0019275597	23.125002	15
CCTCACG	40	0.0019275597	23.125002	2
ATACGGC	40	0.0019275597	23.125002	29
GCATCAC	40	0.0019275597	23.125002	7
CTAATCC	40	0.0019275597	23.125002	3
GATGCTA	65	2.6708258E-6	22.769234	14
GACAGGC	245	0.0	22.65306	7
CCAGGAC	295	0.0	22.576271	3
AGGCCAT	270	0.0	21.925926	25
TAATACT	85	5.1455572E-8	21.764706	4
ATACTGG	120	1.0913936E-11	21.583334	6
ATCAAGC	275	0.0	21.527271	30
>>END_MODULE