FastQCFastQC Report
Fri 10 Feb 2017
ERR1631612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10891
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC320.2938205858047929No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA300.2754567991919934No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA300.2754567991919934No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA280.2570930125791938No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG280.2570930125791938No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT260.2387292259663943No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA240.2203654393535947No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC220.20200165274079515No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA200.1836378661279956No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA200.1836378661279956No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA190.1744559728215958No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA180.16527407951519604No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC170.15609218620879625No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG170.15609218620879625No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG160.14691029290239646No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC150.1377283995959967No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT150.1377283995959967No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG150.1377283995959967No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA140.1285465062895969No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG140.1285465062895969No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT140.1285465062895969No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT140.1285465062895969No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC130.11936461298319714No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA130.11936461298319714No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT130.11936461298319714No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG130.11936461298319714No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA130.11936461298319714No Hit
ACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGAC120.11018271967679735No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG120.11018271967679735No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC120.11018271967679735No Hit
GATCTGCTTGATGGCCTCTTCTGATGCAGCCTGTCCTGGAGCT120.11018271967679735No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC120.11018271967679735No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG120.11018271967679735No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA120.11018271967679735No Hit
CTACTGCAACTAGACGCAGCCTGCAGGCAGCCCCACACCCGCC110.10100082637039758No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG110.10100082637039758No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC110.10100082637039758No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC110.10100082637039758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA251.1549176E-436.9999962
GGTATCA251.1549176E-436.9999961
AACGCAG358.313748E-426.428577
ACGCAGA358.313748E-426.428578
TCAACGC358.313748E-426.428575
TATCAAC358.313748E-426.428573
ATCAACG358.313748E-426.428574
CAACGCA358.313748E-426.428576
AGTACGG400.00181131123.12514
AGAGTAC400.00181131123.12512
GAGTACG400.00181131123.12513
CAGAGTA450.003590509320.55555511
CGCAGAG450.003590509320.5555559
GTACGGG450.003590509320.55555515
GCAGAGT500.006606217518.49999810
Produced by FastQC (version 0.11.5)