##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631612.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10891
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39059774125425	33.0	31.0	34.0	31.0	34.0
2	32.56863465246534	34.0	31.0	34.0	31.0	34.0
3	32.65898448260031	34.0	31.0	34.0	31.0	34.0
4	36.12790377375815	37.0	35.0	37.0	35.0	37.0
5	36.020934716738594	37.0	35.0	37.0	35.0	37.0
6	36.08989073546965	37.0	35.0	37.0	35.0	37.0
7	36.07602607657699	37.0	35.0	37.0	35.0	37.0
8	36.05692773849968	37.0	35.0	37.0	35.0	37.0
9	37.8635570654669	39.0	38.0	39.0	35.0	39.0
10	37.76971811587549	39.0	38.0	39.0	35.0	39.0
11	37.8372968506106	39.0	38.0	39.0	35.0	39.0
12	37.7454779175466	39.0	38.0	39.0	35.0	39.0
13	37.83022679276467	39.0	38.0	39.0	35.0	39.0
14	39.15884675420072	40.0	39.0	41.0	36.0	41.0
15	39.14241116518226	40.0	39.0	41.0	36.0	41.0
16	39.081810669360024	40.0	38.0	41.0	36.0	41.0
17	39.00367275732256	40.0	38.0	41.0	36.0	41.0
18	39.0321366265724	40.0	38.0	41.0	36.0	41.0
19	39.040124873748965	40.0	39.0	41.0	36.0	41.0
20	39.04554219079974	40.0	39.0	41.0	36.0	41.0
21	38.97888164539528	40.0	38.0	41.0	35.0	41.0
22	38.98677807363878	40.0	38.0	41.0	35.0	41.0
23	38.90707923973923	40.0	38.0	41.0	35.0	41.0
24	38.92838123221008	40.0	38.0	41.0	35.0	41.0
25	38.82517675144615	40.0	38.0	41.0	35.0	41.0
26	38.72518593333945	40.0	38.0	41.0	35.0	41.0
27	38.65154714902213	40.0	38.0	41.0	35.0	41.0
28	38.68496924065742	40.0	38.0	41.0	35.0	41.0
29	38.649343494628596	40.0	38.0	41.0	35.0	41.0
30	38.60885134514737	40.0	38.0	41.0	35.0	41.0
31	38.488201267101275	40.0	38.0	41.0	34.0	41.0
32	38.43779267284914	40.0	38.0	41.0	34.0	41.0
33	38.408869708933985	40.0	38.0	41.0	34.0	41.0
34	38.373703057570474	40.0	38.0	41.0	34.0	41.0
35	38.27031493894041	40.0	38.0	41.0	34.0	41.0
36	38.202460747406114	40.0	38.0	41.0	34.0	41.0
37	38.121660086309795	40.0	37.0	41.0	34.0	41.0
38	38.124598292167846	40.0	37.0	41.0	33.0	41.0
39	38.05242861077954	40.0	37.0	41.0	33.0	41.0
40	37.73161325865394	40.0	37.0	41.0	33.0	41.0
41	37.83298136075659	40.0	37.0	41.0	33.0	41.0
42	37.83738866954366	40.0	37.0	41.0	33.0	41.0
43	37.34230098246258	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	2.0
23	4.0
24	13.0
25	11.0
26	23.0
27	35.0
28	36.0
29	88.0
30	88.0
31	126.0
32	164.0
33	250.0
34	363.0
35	569.0
36	702.0
37	1348.0
38	2825.0
39	4242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23753557983656	15.315398035074832	12.817923055734093	28.629143329354513
2	21.320356257460286	18.887154531264347	31.760168946836835	28.032320264438525
3	21.164264071251495	19.392158663116334	28.812781195482508	30.630796070149664
4	16.20604168579561	15.049123129189237	32.36617390505923	36.37866127995593
5	17.656780828206774	32.64163070425122	32.93545129005601	16.766137177485998
6	35.148287576898355	32.797722890460015	14.801212009916446	17.252777522725186
7	31.20007345514645	26.46221650904417	20.246074740611515	22.09163529519787
8	28.151684877421722	30.061518685152876	19.805343861904326	21.981452575521075
9	28.399595996694522	12.221099990818107	17.583325681755575	41.7959783307318
10	20.47562207327151	23.75355798365623	27.802772931778534	27.968047011293727
11	39.82187126985585	18.161784960058764	17.91387384078597	24.10246992929942
12	23.615829584060233	22.679276466807455	25.902121017353778	27.802772931778534
13	35.093196217059955	16.389679551923606	20.383803140207508	28.133321090808927
14	24.313653475346616	19.079974290698743	22.59663942704986	34.009732806904786
15	30.860343402809658	22.743549719952256	19.42888623634193	26.96722064089615
16	26.34285189606097	22.734367826645855	20.7694426590763	30.153337618216874
17	27.389587732990545	23.900468276558627	20.622532366173903	28.087411624276925
18	27.279405013313745	20.273620420530712	22.312000734551464	30.13497383160408
19	28.941327701772106	21.64172252318428	21.67845009640988	27.738499678633733
20	30.07070057845928	20.907171058672297	21.63254062987788	27.389587732990545
21	29.91460839225048	20.236892847305114	20.7969883389955	29.0515104214489
22	30.55734092369847	22.174272334955468	19.25443026352034	28.01395647782573
23	29.91460839225048	22.12836286842347	20.08998255440272	27.867046184923332
24	28.629143329354513	20.640896152786706	21.118354604719496	29.61160591313929
25	28.133321090808927	21.568267376733083	22.192636121568267	28.105775410889727
26	28.831144982095307	22.660912680194656	21.73354145624828	26.77440088146176
27	27.912955651455327	20.705169405931503	23.726012303737033	27.655862638876137
28	27.582407492424938	21.60499494995868	21.338720044073085	29.473877513543297
29	28.555688182903317	22.57827564043706	21.834542282618678	27.031493894040953
30	27.371223946377743	21.035717564961896	23.606647690753835	27.98641079790653
31	28.24350381048572	21.917179322376274	22.275273161325863	27.564043705812136
32	26.21430538977137	21.66008630979708	22.51400238729226	29.61160591313929
33	26.884583601138555	21.58663116334588	23.560738224221833	27.968047011293727
34	27.766045358552933	22.293636947938662	23.147553025433844	26.792764668074557
35	27.279405013313745	22.42218345422826	23.77192177026903	26.526489762188966
36	27.508952345973743	21.834542282618678	22.10081718850427	28.555688182903317
37	26.480580295656964	21.74272334955468	23.872922596639427	27.90377375814893
38	26.535671655495364	20.8704434854467	24.827839500505004	27.766045358552933
39	25.222660912680194	20.35625746028831	25.892939124047377	28.528142502984117
40	25.268570379212196	21.21935543108989	26.811128454687356	26.700945735010563
41	24.10246992929942	20.438894500045908	27.196767973556145	28.261867597098522
42	22.357910201083463	20.751078872463502	29.1341474612065	27.756863465246536
43	22.615003213662657	20.677623726012303	28.24350381048572	28.46386924983932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.0
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	2.0
13	2.0
14	2.0
15	7.5
16	13.0
17	14.0
18	15.0
19	15.0
20	17.5
21	20.0
22	19.5
23	19.0
24	17.5
25	16.0
26	16.0
27	14.5
28	13.0
29	21.5
30	30.0
31	42.0
32	54.0
33	54.0
34	67.0
35	80.0
36	108.5
37	137.0
38	162.5
39	188.0
40	188.0
41	246.5
42	305.0
43	382.5
44	460.0
45	607.5
46	755.0
47	755.0
48	797.0
49	839.0
50	1010.0
51	1181.0
52	1197.5
53	1214.0
54	1214.0
55	1140.5
56	1067.0
57	1014.5
58	962.0
59	903.0
60	844.0
61	844.0
62	834.0
63	824.0
64	644.0
65	464.0
66	420.0
67	376.0
68	376.0
69	309.0
70	242.0
71	242.5
72	243.0
73	254.0
74	265.0
75	265.0
76	207.5
77	150.0
78	102.5
79	55.0
80	40.5
81	26.0
82	26.0
83	18.0
84	10.0
85	10.5
86	11.0
87	8.0
88	5.0
89	5.0
90	3.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	10891.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.85914975667983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.78518150174042	64.09879717197686
2	6.887120835405271	10.173537783490955
3	2.449030333167578	5.42649894408227
4	1.2182993535554452	3.5993021761087136
5	0.6713078070611637	2.4791111927279403
6	0.5718547986076579	2.534202552566339
7	0.34808552958727	1.7996510880543568
8	0.174042764793635	1.0283720503167753
9	0.19890601690701143	1.3221926361215683
>10	0.69617105917454	7.538334404554218
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	32	0.2938205858047929	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	30	0.2754567991919934	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	30	0.2754567991919934	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	28	0.2570930125791938	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	28	0.2570930125791938	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	26	0.2387292259663943	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	24	0.2203654393535947	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	22	0.20200165274079515	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	20	0.1836378661279956	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	20	0.1836378661279956	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	19	0.1744559728215958	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	18	0.16527407951519604	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	17	0.15609218620879625	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	17	0.15609218620879625	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	16	0.14691029290239646	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	15	0.1377283995959967	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	15	0.1377283995959967	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	15	0.1377283995959967	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	14	0.1285465062895969	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	14	0.1285465062895969	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	14	0.1285465062895969	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	14	0.1285465062895969	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	13	0.11936461298319714	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	13	0.11936461298319714	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	13	0.11936461298319714	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	13	0.11936461298319714	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	13	0.11936461298319714	No Hit
ACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGAC	12	0.11018271967679735	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	12	0.11018271967679735	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	12	0.11018271967679735	No Hit
GATCTGCTTGATGGCCTCTTCTGATGCAGCCTGTCCTGGAGCT	12	0.11018271967679735	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	12	0.11018271967679735	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	12	0.11018271967679735	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	12	0.11018271967679735	No Hit
CTACTGCAACTAGACGCAGCCTGCAGGCAGCCCCACACCCGCC	11	0.10100082637039758	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	11	0.10100082637039758	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	11	0.10100082637039758	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	11	0.10100082637039758	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.009181893306399779	0.0
23	0.0	0.0	0.0	0.009181893306399779	0.0
24	0.0	0.0	0.0	0.009181893306399779	0.0
25	0.0	0.0	0.0	0.009181893306399779	0.0
26	0.0	0.0	0.0	0.018363786612799558	0.0
27	0.0	0.0	0.0	0.027545679919199338	0.0
28	0.0	0.0	0.0	0.06427325314479845	0.0
29	0.0	0.0	0.0	0.2295473326599945	0.0
30	0.0	0.0	0.0	0.3397300523367918	0.0
31	0.0	0.0	0.0	0.6427325314479846	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	25	1.1549176E-4	36.999996	2
GGTATCA	25	1.1549176E-4	36.999996	1
AACGCAG	35	8.313748E-4	26.42857	7
ACGCAGA	35	8.313748E-4	26.42857	8
TCAACGC	35	8.313748E-4	26.42857	5
TATCAAC	35	8.313748E-4	26.42857	3
ATCAACG	35	8.313748E-4	26.42857	4
CAACGCA	35	8.313748E-4	26.42857	6
AGTACGG	40	0.001811311	23.125	14
AGAGTAC	40	0.001811311	23.125	12
GAGTACG	40	0.001811311	23.125	13
CAGAGTA	45	0.0035905093	20.555555	11
CGCAGAG	45	0.0035905093	20.555555	9
GTACGGG	45	0.0035905093	20.555555	15
GCAGAGT	50	0.0066062175	18.499998	10
>>END_MODULE