ERR1631611.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1631611.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28275
Sequences flagged as poor quality	0
Sequence length	43
%GC	53

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	104	0.367816091954023	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	77	0.2723253757736516	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	76	0.268788682581786	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	73	0.2581786030061892	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	66	0.23342175066312998	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	63	0.22281167108753316	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	60	0.21220159151193632	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	59	0.20866489832007074	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	57	0.2015915119363395	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	57	0.2015915119363395	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	55	0.1945181255526083	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	54	0.1909814323607427	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	49	0.17329796640141468	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	46	0.16268788682581786	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	45	0.15915119363395225	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	45	0.15915119363395225	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	43	0.15207780725022105	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	42	0.14854111405835543	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	41	0.14500442086648982	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	40	0.14146772767462423	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	38	0.134394341290893	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	37	0.13085764809902742	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	36	0.1273209549071618	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	34	0.12024756852343059	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	34	0.12024756852343059	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	33	0.11671087533156499	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	32	0.11317418213969938	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	32	0.11317418213969938	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	32	0.11317418213969938	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	32	0.11317418213969938	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	32	0.11317418213969938	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	31	0.10963748894783377	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	31	0.10963748894783377	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	31	0.10963748894783377	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	31	0.10963748894783377	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29	0.10256410256410256	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	29	0.10256410256410256	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	29	0.10256410256410256	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCTCA	20	0.0018117158	37.0	29
CGAGCTC	20	0.0018117158	37.0	28
TCATCGA	20	0.0018117158	37.0	24
ATCGAGC	20	0.0018117158	37.0	26
TCTTATA	30	8.028472E-6	37.0	36
TCGAGCT	20	0.0018117158	37.0	27
CTTATAC	35	2.3074872E-5	31.714285	37
GCTCACA	25	0.0054071904	29.6	31
CATCGAG	25	0.0054071904	29.6	25
ATCAACG	65	8.73315E-8	25.615385	2
TCAACGC	70	1.788776E-7	23.785713	3
TATCAAC	70	1.788776E-7	23.785713	1
GTCCCGC	40	0.0018852013	23.125002	12
CTGTGAG	40	0.0018852013	23.125002	1
AACGCAG	75	3.4812183E-7	22.199999	5
CGCAGAG	80	6.479804E-7	20.8125	7
CAACGCA	80	6.479804E-7	20.8125	4
CGTCATC	45	0.0037355674	20.555555	2
CCGTCAT	45	0.0037355674	20.555555	1
TCCCGCC	45	0.0037355674	20.555555	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content