##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631611.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28275
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56679045092838	34.0	31.0	34.0	31.0	34.0
2	32.737577365163574	34.0	31.0	34.0	31.0	34.0
3	32.83023872679045	34.0	31.0	34.0	31.0	34.0
4	36.27140583554377	37.0	37.0	37.0	35.0	37.0
5	36.16367816091954	37.0	35.0	37.0	35.0	37.0
6	36.2390450928382	37.0	37.0	37.0	35.0	37.0
7	36.21068081343943	37.0	37.0	37.0	35.0	37.0
8	36.19473032714412	37.0	36.0	37.0	35.0	37.0
9	38.03328028293546	39.0	38.0	39.0	35.0	39.0
10	37.954942528735636	39.0	38.0	39.0	35.0	39.0
11	38.02953138815208	39.0	38.0	39.0	35.0	39.0
12	37.95900972590628	39.0	38.0	39.0	35.0	39.0
13	38.02818744473917	39.0	38.0	39.0	35.0	39.0
14	39.39579133510168	40.0	39.0	41.0	37.0	41.0
15	39.41078691423519	40.0	39.0	41.0	37.0	41.0
16	39.332838196286474	40.0	39.0	41.0	36.0	41.0
17	39.31685234305924	40.0	39.0	41.0	36.0	41.0
18	39.32760389036251	40.0	39.0	41.0	36.0	41.0
19	39.347197170645444	40.0	39.0	41.0	36.0	41.0
20	39.36969053934571	40.0	39.0	41.0	36.0	41.0
21	39.31755968169761	40.0	39.0	41.0	36.0	41.0
22	39.3306100795756	40.0	39.0	41.0	36.0	41.0
23	39.258320070733866	40.0	39.0	41.0	36.0	41.0
24	39.24993810786914	40.0	39.0	41.0	36.0	41.0
25	39.19013262599469	40.0	39.0	41.0	36.0	41.0
26	39.12756852343059	40.0	39.0	41.0	36.0	41.0
27	39.00049513704686	40.0	39.0	41.0	35.0	41.0
28	38.996923076923075	40.0	39.0	41.0	35.0	41.0
29	38.96944297082228	40.0	39.0	41.0	35.0	41.0
30	38.947550839964634	40.0	38.0	41.0	35.0	41.0
31	38.87140583554377	40.0	38.0	41.0	35.0	41.0
32	38.80272325375773	40.0	38.0	41.0	35.0	41.0
33	38.6959151193634	40.0	38.0	41.0	35.0	41.0
34	38.70935455349248	40.0	38.0	41.0	35.0	41.0
35	38.607745358090185	40.0	38.0	41.0	35.0	41.0
36	38.59278514588859	40.0	38.0	41.0	35.0	41.0
37	38.51366931918656	40.0	38.0	41.0	34.0	41.0
38	38.47688770999116	40.0	38.0	41.0	34.0	41.0
39	38.427126436781606	40.0	38.0	41.0	34.0	41.0
40	38.10083112290009	40.0	37.0	41.0	34.0	41.0
41	38.17633952254642	40.0	37.0	41.0	34.0	41.0
42	38.17442970822281	40.0	37.0	41.0	34.0	41.0
43	37.68816976127321	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	3.0
23	9.0
24	21.0
25	26.0
26	44.0
27	73.0
28	93.0
29	164.0
30	207.0
31	268.0
32	407.0
33	519.0
34	689.0
35	1101.0
36	1688.0
37	2885.0
38	6732.0
39	13344.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.67020335985853	16.222811671087534	12.61892130857648	28.488063660477454
2	21.503094606542884	19.41998231653404	31.4447391688771	27.632183908045977
3	21.273209549071616	19.356321839080458	29.598585322723252	29.771883289124666
4	15.791335101679929	16.09549071618037	34.281167108753316	33.832007073386386
5	16.456233421750664	33.36870026525199	33.77188328912467	16.40318302387268
6	33.764809902740936	34.01237842617153	15.992926613616268	16.229885057471265
7	29.485411140583555	28.15207780725022	20.350132625994696	22.01237842617153
8	27.151193633952253	30.15030946065429	21.46419098143236	21.2343059239611
9	27.883289124668437	12.717948717948719	17.888594164456233	41.51016799292661
10	18.71264367816092	24.0106100795756	29.050397877984086	28.226348364279396
11	39.51547303271441	19.16180371352785	18.41556145004421	22.907161803713528
12	22.48983200707339	24.099027409372237	26.46861184792219	26.94252873563218
13	35.826702033598586	17.163572060123784	20.703801945181254	26.305923961096372
14	24.08841732979664	20.045977011494255	23.310344827586206	32.5552608311229
15	30.015915119363395	24.282935455349246	19.526083112290006	26.175066312997348
16	25.6657824933687	24.08841732979664	22.42970822281167	27.816091954022987
17	27.38107869142352	23.69584438549956	21.411140583554378	27.511936339522546
18	26.27055702917772	21.227232537577365	23.45181255526083	29.050397877984086
19	27.936339522546422	22.702033598585324	23.221927497789565	26.13969938107869
20	30.836427939876216	20.49160035366932	22.12555260831123	26.546419098143236
21	27.218390804597703	22.305923961096376	21.386383731211318	29.089301503094607
22	28.647214854111407	22.578249336870027	20.841732979664016	27.932802829354554
23	28.357206012378427	22.03359858532272	21.870910698496907	27.738284703801945
24	27.78779840848806	21.616268788682582	22.528735632183906	28.067197170645446
25	27.709991158267023	22.171529619805483	23.285587975243146	26.832891246684348
26	28.021220159151195	23.05923961096375	21.78603006189213	27.133510167992924
27	26.69849690539346	22.358974358974358	24.081343943412907	26.861184792219273
28	25.892130857648098	22.80813439434129	22.157382847038022	29.142351900972592
29	27.632183908045977	23.713527851458885	21.77188328912467	26.88240495137047
30	27.33863837312113	22.104332449160037	23.45181255526083	27.105216622458002
31	28.799292661361626	22.450928381962864	22.05128205128205	26.69849690539346
32	25.81078691423519	21.832007073386382	23.020335985853226	29.336870026525197
33	25.800176834659595	22.09372236958444	24.636604774535808	27.46949602122016
34	26.274093722369585	21.411140583554378	23.876215738284703	28.438549955791338
35	25.450044208664895	24.02475685234306	24.572944297082227	25.952254641909818
36	26.7763041556145	22.71264367816092	24.378426171529618	26.13262599469496
37	27.607427055702914	22.387267904509283	23.536693191865606	26.46861184792219
38	25.959328028293545	22.404951370468613	25.124668435013263	26.51105216622458
39	25.598585322723256	21.220159151193634	25.761273209549074	27.419982316534043
40	25.09637488947834	22.436781609195403	26.645446507515473	25.821396993810787
41	23.101679929266137	21.08576480990274	27.752431476569406	28.060123784261716
42	22.288240495137046	21.44650751547303	28.85234305923961	27.41290893015031
43	21.55968169761273	22.302387267904507	28.290008841732977	27.84792219274978
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.5
7	2.0
8	2.0
9	2.0
10	2.0
11	2.0
12	2.0
13	8.5
14	15.0
15	29.5
16	44.0
17	47.0
18	50.0
19	50.0
20	52.5
21	55.0
22	40.5
23	26.0
24	42.5
25	59.0
26	59.0
27	63.5
28	68.0
29	108.5
30	149.0
31	180.0
32	211.0
33	211.0
34	259.0
35	307.0
36	345.0
37	383.0
38	489.0
39	595.0
40	595.0
41	708.0
42	821.0
43	951.5
44	1082.0
45	1575.5
46	2069.0
47	2069.0
48	2345.0
49	2621.0
50	3015.5
51	3410.0
52	3492.0
53	3574.0
54	3574.0
55	3200.5
56	2827.0
57	2653.5
58	2480.0
59	2325.0
60	2170.0
61	2170.0
62	1994.0
63	1818.0
64	1487.0
65	1156.0
66	972.5
67	789.0
68	789.0
69	650.5
70	512.0
71	463.0
72	414.0
73	321.5
74	229.0
75	229.0
76	181.5
77	134.0
78	107.5
79	81.0
80	65.5
81	50.0
82	50.0
83	43.0
84	36.0
85	26.5
86	17.0
87	13.5
88	10.0
89	10.0
90	7.0
91	4.0
92	2.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	28275.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.05305039787798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.98160274399751	59.19363395225464
2	6.189585282195198	8.424403183023871
3	2.385406922357343	4.870026525198939
4	1.3044382080864776	3.550839964633068
5	0.8471052905103419	2.882404951370469
6	0.4781207774659599	1.9522546419098143
7	0.2806361085126286	1.336870026525199
8	0.25984824862280426	1.414677276746242
9	0.2494543186778921	1.5278514588859415
>10	0.9614385199043759	12.014146772767463
>50	0.05716661469701694	2.465075154730327
>100	0.005196964972456086	0.367816091954023
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	104	0.367816091954023	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	77	0.2723253757736516	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	76	0.268788682581786	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	73	0.2581786030061892	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	66	0.23342175066312998	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	63	0.22281167108753316	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	60	0.21220159151193632	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	59	0.20866489832007074	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	57	0.2015915119363395	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	57	0.2015915119363395	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	55	0.1945181255526083	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	54	0.1909814323607427	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	49	0.17329796640141468	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	46	0.16268788682581786	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	45	0.15915119363395225	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	45	0.15915119363395225	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	43	0.15207780725022105	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	42	0.14854111405835543	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	41	0.14500442086648982	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	40	0.14146772767462423	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	38	0.134394341290893	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	37	0.13085764809902742	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	36	0.1273209549071618	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	34	0.12024756852343059	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	34	0.12024756852343059	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	33	0.11671087533156499	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	32	0.11317418213969938	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	32	0.11317418213969938	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	32	0.11317418213969938	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	32	0.11317418213969938	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	32	0.11317418213969938	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	31	0.10963748894783377	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	31	0.10963748894783377	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	31	0.10963748894783377	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	31	0.10963748894783377	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29	0.10256410256410256	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	29	0.10256410256410256	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	29	0.10256410256410256	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0035366931918656055	0.0
23	0.0	0.0	0.0	0.0035366931918656055	0.0
24	0.0	0.0	0.0	0.0035366931918656055	0.0
25	0.0	0.0	0.0	0.0035366931918656055	0.0
26	0.0	0.0	0.0	0.0035366931918656055	0.0
27	0.0	0.0	0.0	0.01768346595932803	0.0
28	0.0	0.0	0.0	0.08488063660477453	0.0
29	0.0	0.0	0.0	0.23342175066312998	0.0
30	0.0	0.0	0.0	0.41025641025641024	0.0
31	0.0	0.0	0.0	0.8841732979664014	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCTCA	20	0.0018117158	37.0	29
CGAGCTC	20	0.0018117158	37.0	28
TCATCGA	20	0.0018117158	37.0	24
ATCGAGC	20	0.0018117158	37.0	26
TCTTATA	30	8.028472E-6	37.0	36
TCGAGCT	20	0.0018117158	37.0	27
CTTATAC	35	2.3074872E-5	31.714285	37
GCTCACA	25	0.0054071904	29.6	31
CATCGAG	25	0.0054071904	29.6	25
ATCAACG	65	8.73315E-8	25.615385	2
TCAACGC	70	1.788776E-7	23.785713	3
TATCAAC	70	1.788776E-7	23.785713	1
GTCCCGC	40	0.0018852013	23.125002	12
CTGTGAG	40	0.0018852013	23.125002	1
AACGCAG	75	3.4812183E-7	22.199999	5
CGCAGAG	80	6.479804E-7	20.8125	7
CAACGCA	80	6.479804E-7	20.8125	4
CGTCATC	45	0.0037355674	20.555555	2
CCGTCAT	45	0.0037355674	20.555555	1
TCCCGCC	45	0.0037355674	20.555555	13
>>END_MODULE