FastQCFastQC Report
Fri 10 Feb 2017
ERR1631609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410980
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44391.0801012214706311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37960.9236459195094651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32030.7793566596914692No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.41340211202491606No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA7850.19100686164776876No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6480.157671906175483No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC6260.1523188476324882No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.1457491848751764No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5800.14112608886077183No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC5770.14039612633218163No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC5280.12847340503187504No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG5070.12336366733174363No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC4820.11728064626015863No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG4810.11703732541729524No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.11582072120297825No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.11533407951725146No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.11363083361720765No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT4340.10560124580271546No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG4280.10414132074553506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA405.930987E-527.7500023
GTACTAT502.698011E-422.1999991
TCTAGCG1301.8189894E-1221.34615328
ATTCGGC450.003821689220.55555520
CTAGCGG1351.8189894E-1220.55555329
TAAGCAG555.1353686E-420.1818185
TACTATA656.888348E-519.9230772
GTTTTCG1052.2488166E-819.3809528
CAGCGTC609.2230586E-418.52
TTCAGGA701.2167798E-418.52
GCAGCGT609.2230586E-418.51
ACCTCTA1552.0008883E-1117.90322725
CGGTCCA1451.546141E-1017.86206810
TTAGAGT1258.53106E-917.7600024
CCGGTCC1502.4920155E-1017.2666669
ATACACT650.001577599817.0769234
GAGTGTT1201.0363692E-716.9583327
TAGAGTG1201.0363692E-716.9583325
GGTTTTC1201.0363692E-716.95833227
AGAATTT1655.2750693E-1116.81818217
Produced by FastQC (version 0.11.5)