##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631609.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410980
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01852401576719	34.0	33.0	34.0	31.0	34.0
2	33.1317728356611	34.0	33.0	34.0	31.0	34.0
3	33.197532726653364	34.0	33.0	34.0	31.0	34.0
4	36.44356416370626	37.0	37.0	37.0	35.0	37.0
5	36.43968806267945	37.0	37.0	37.0	35.0	37.0
6	36.50822181128036	37.0	37.0	37.0	35.0	37.0
7	36.50990315830454	37.0	37.0	37.0	35.0	37.0
8	36.52707917660227	37.0	37.0	37.0	35.0	37.0
9	38.3600613168524	39.0	39.0	39.0	37.0	39.0
10	38.33465862085746	39.0	39.0	39.0	37.0	39.0
11	38.374517008126915	39.0	39.0	39.0	37.0	39.0
12	38.361981118302594	39.0	39.0	39.0	37.0	39.0
13	38.38565866952163	39.0	39.0	39.0	37.0	39.0
14	39.88454912647818	41.0	40.0	41.0	38.0	41.0
15	39.852080393206485	41.0	40.0	41.0	38.0	41.0
16	39.78433987055331	41.0	40.0	41.0	38.0	41.0
17	39.8292739306049	41.0	40.0	41.0	38.0	41.0
18	39.8144459584408	41.0	40.0	41.0	38.0	41.0
19	39.832848313786556	41.0	40.0	41.0	38.0	41.0
20	39.79826755559881	41.0	40.0	41.0	38.0	41.0
21	39.76859944522848	41.0	40.0	41.0	37.0	41.0
22	39.68372426882087	41.0	40.0	41.0	37.0	41.0
23	39.60217285512677	41.0	40.0	41.0	37.0	41.0
24	39.54387804759356	41.0	40.0	41.0	37.0	41.0
25	39.54291206384739	41.0	40.0	41.0	36.0	41.0
26	39.40635554041559	41.0	39.0	41.0	36.0	41.0
27	39.31003941797655	41.0	39.0	41.0	35.0	41.0
28	39.182441967978974	41.0	39.0	41.0	35.0	41.0
29	39.075439194121365	41.0	39.0	41.0	35.0	41.0
30	38.94135481045306	41.0	38.0	41.0	35.0	41.0
31	38.81176699596087	40.0	38.0	41.0	35.0	41.0
32	38.66690106574529	40.0	38.0	41.0	35.0	41.0
33	38.51029733806998	40.0	38.0	41.0	35.0	41.0
34	38.4091439972748	40.0	38.0	41.0	35.0	41.0
35	38.251184972504745	40.0	37.0	41.0	35.0	41.0
36	38.12018103070709	40.0	37.0	41.0	35.0	41.0
37	37.98182393303811	40.0	37.0	41.0	35.0	41.0
38	37.83511606404205	40.0	37.0	41.0	34.0	41.0
39	37.65837996982822	40.0	36.0	41.0	34.0	41.0
40	37.45861355783737	40.0	35.0	41.0	33.0	41.0
41	37.2516229500219	40.0	35.0	41.0	33.0	41.0
42	36.98233004039126	40.0	35.0	41.0	33.0	41.0
43	36.136510292471655	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	2.0
14	3.0
15	3.0
16	1.0
17	1.0
18	9.0
19	10.0
20	26.0
21	76.0
22	125.0
23	211.0
24	364.0
25	589.0
26	994.0
27	1358.0
28	2057.0
29	2882.0
30	3575.0
31	4174.0
32	5137.0
33	6310.0
34	9299.0
35	12910.0
36	20498.0
37	46027.0
38	76563.0
39	217774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.18492384057618	17.9144970558178	13.150031631709572	23.750547471896443
2	19.875419728453938	21.888656382305708	32.297435398316225	25.938488490924133
3	21.098836926371114	23.803348094797798	28.75127743442503	26.34653754440605
4	14.933816730741157	16.52221519295343	31.65117523967103	36.892792836634385
5	16.04214316998394	35.59443281911528	30.87108861745097	17.492335393449803
6	33.22765098058299	32.606939510438465	16.58937174558373	17.57603776339481
7	28.465375444060538	30.66767239281717	20.646503479488054	20.22044868363424
8	29.30653559783931	30.04136454328678	19.846464548153193	20.805635310720717
9	27.514964231836096	13.939607766801304	20.478368777069445	38.06705922429315
10	20.397586257238796	25.515596866027547	28.045646990121178	26.041169886612487
11	35.56280110954304	22.725436760912938	19.168816000778627	22.54294612876539
12	22.859749866173537	27.62518857365322	25.900773760280305	23.61428779989294
13	30.79395591026327	18.667575064480026	25.18103070709037	25.357438318166338
14	23.867828118156602	21.878193586062583	24.43184583191396	29.822132463866858
15	27.322984086816877	26.41637062630785	22.16652878485571	24.094116502019563
16	24.750596136065013	25.276412477492823	24.979561049199475	24.99343033724269
17	23.674631368923063	27.150712930069588	24.555939461774294	24.618716239233052
18	23.846659204827485	22.988953233734	27.649034016253832	25.51535354518468
19	23.539831621976738	25.931188865638234	27.365808555160836	23.163170957224196
20	24.276850455009978	23.673171443865883	27.809869093386542	24.240109007737605
21	26.86894739403377	24.278310380067154	26.804710691517837	22.048031534381234
22	26.06720521679887	24.256168183366587	25.948707966324395	23.727918633510146
23	24.130614628449074	24.56761886223174	26.887683098934257	24.414083410384933
24	23.367317144386586	26.256022190860868	26.89108959073434	23.4855710740182
25	23.476568202832254	24.86252372378218	26.805440654046425	24.855467419339142
26	24.175385663535938	25.88690447223709	26.780865248917223	23.156844615309748
27	24.74232322740766	24.36152610832644	26.51807873862475	24.37807192564115
28	22.93396272324687	25.29709474913621	28.35320453550051	23.415737992116405
29	23.014745243077524	25.286875273735948	28.45418268528882	23.244196797897708
30	22.29013577303032	26.46649471993771	28.55856732687722	22.684802180154755
31	23.740084675653318	26.020487614969095	26.274514574918488	23.9649131344591
32	22.404983210861843	25.591756289843786	27.26823689717261	24.735023602121757
33	21.882573361234122	24.9255438220838	28.43277045111684	24.759112365565233
34	23.452966081074507	25.195873278505037	27.350965983746168	24.00019465667429
35	23.108180446737066	24.884422599639887	28.331792301328534	23.675604652294517
36	21.118545914643047	25.87595503430824	27.916200301717847	25.08929874933087
37	22.39768358557594	24.560805878631562	28.771473064382697	24.2700374714098
38	21.523918438853475	23.255876198355153	29.900481775268872	25.319723587522507
39	21.901065745291742	23.187989683196264	30.055477152172855	24.855467419339142
40	21.1122195727286	23.999708014988563	30.375444060538225	24.51262835174461
41	20.055720473015718	24.60144045938975	31.238989731860435	24.103849335734097
42	20.81293493600662	24.976641199085115	30.694681006375006	23.51574285853326
43	19.44814832838581	24.08462698914789	30.77862669716288	25.688597985303417
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	84.0
2	124.0
3	519.5
4	915.0
5	915.0
6	1143.0
7	1371.0
8	1350.5
9	1330.0
10	1873.5
11	2417.0
12	2417.0
13	4129.5
14	5842.0
15	8142.5
16	10443.0
17	9153.5
18	7864.0
19	7864.0
20	8068.0
21	8272.0
22	5964.0
23	3656.0
24	3537.5
25	3419.0
26	3419.0
27	3643.0
28	3867.0
29	4312.5
30	4758.0
31	5474.0
32	6190.0
33	6190.0
34	7306.5
35	8423.0
36	10274.0
37	12125.0
38	14001.0
39	15877.0
40	15877.0
41	17760.0
42	19643.0
43	21333.5
44	23024.0
45	25603.0
46	28182.0
47	28182.0
48	30397.0
49	32612.0
50	31643.0
51	30674.0
52	29714.5
53	28755.0
54	28755.0
55	27393.5
56	26032.0
57	25101.5
58	24171.0
59	23476.0
60	22781.0
61	22781.0
62	21385.5
63	19990.0
64	18748.0
65	17506.0
66	15520.0
67	13534.0
68	13534.0
69	11531.0
70	9528.0
71	8250.5
72	6973.0
73	5150.0
74	3327.0
75	3327.0
76	2659.0
77	1991.0
78	1732.0
79	1473.0
80	1401.5
81	1330.0
82	1330.0
83	1049.0
84	768.0
85	764.0
86	760.0
87	706.0
88	652.0
89	652.0
90	466.5
91	281.0
92	159.5
93	38.0
94	24.5
95	11.0
96	11.0
97	8.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	410980.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.30629391254714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.93371134894255	45.62859133202476
2	5.287655502704587	5.425800146601175
3	1.670768413442826	2.5716280583969313
4	0.8883502649888542	1.8231183917122917
5	0.5174309349203569	1.3273731813233942
6	0.3542948627211284	1.090653381508545
7	0.2787891359696185	1.001254614477762
8	0.19079149544721025	0.7831043633143174
9	0.16211289461333553	0.7485670636241017
>10	1.3699106527487188	14.463063432670332
>50	0.19502680143504328	7.092344949591807
>100	0.14543563432197346	13.644017862667464
>500	0.0038147051625435657	1.1865427045712247
>1k	0.0019073525812717828	3.2139405175159133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4439	1.0801012214706311	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3796	0.9236459195094651	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3203	0.7793566596914692	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1699	0.41340211202491606	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	785	0.19100686164776876	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	648	0.157671906175483	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	626	0.1523188476324882	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.1457491848751764	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	580	0.14112608886077183	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	577	0.14039612633218163	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	528	0.12847340503187504	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	507	0.12336366733174363	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	482	0.11728064626015863	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	481	0.11703732541729524	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	476	0.11582072120297825	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	474	0.11533407951725146	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	467	0.11363083361720765	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	434	0.10560124580271546	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	428	0.10414132074553506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	2.4332084286339967E-4	0.0	0.0	0.0
11	0.0	2.4332084286339967E-4	0.0	0.0014599250571803982	0.0
12	0.0	2.4332084286339967E-4	0.0	0.0031631709572241956	0.0
13	0.0	2.4332084286339967E-4	0.0	0.004379775171541194	0.0
14	0.0	2.4332084286339967E-4	0.0	0.006083021071584992	0.0
15	0.0	2.4332084286339967E-4	0.0	0.00802958781449219	0.0
16	0.0	2.4332084286339967E-4	0.0	0.018735704900481775	0.0
17	0.0	2.4332084286339967E-4	0.0	0.027738576086427564	0.0
18	0.0	2.4332084286339967E-4	0.0	0.03090174704365176	0.0
19	0.0	2.4332084286339967E-4	0.0	0.04087790160105115	0.0
20	0.0	2.4332084286339967E-4	0.0	0.049637451944133534	0.0
21	0.0	2.4332084286339967E-4	0.0	0.06229013577303032	0.0
22	0.0	2.4332084286339967E-4	0.0	0.0793225947734683	0.0
23	0.0	2.4332084286339967E-4	0.0	0.10389799990267166	0.0
24	0.0	2.4332084286339967E-4	0.0	0.140639447175045	0.0
25	0.0	2.4332084286339967E-4	0.0	0.1542654143753954	0.0
26	0.0	2.4332084286339967E-4	0.0	0.18078738624750595	0.0
27	0.0	2.4332084286339967E-4	0.0	0.2525670348922089	0.0
28	0.0	2.4332084286339967E-4	0.0	0.4357876295683488	0.0
29	0.0	2.4332084286339967E-4	0.0	0.6645092218599445	0.0
30	0.0	2.4332084286339967E-4	0.0	1.022677502554869	0.0
31	0.0	2.4332084286339967E-4	0.0	1.8205265463039564	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCA	40	5.930987E-5	27.750002	3
GTACTAT	50	2.698011E-4	22.199999	1
TCTAGCG	130	1.8189894E-12	21.346153	28
ATTCGGC	45	0.0038216892	20.555555	20
CTAGCGG	135	1.8189894E-12	20.555553	29
TAAGCAG	55	5.1353686E-4	20.181818	5
TACTATA	65	6.888348E-5	19.923077	2
GTTTTCG	105	2.2488166E-8	19.38095	28
CAGCGTC	60	9.2230586E-4	18.5	2
TTCAGGA	70	1.2167798E-4	18.5	2
GCAGCGT	60	9.2230586E-4	18.5	1
ACCTCTA	155	2.0008883E-11	17.903227	25
CGGTCCA	145	1.546141E-10	17.862068	10
TTAGAGT	125	8.53106E-9	17.760002	4
CCGGTCC	150	2.4920155E-10	17.266666	9
ATACACT	65	0.0015775998	17.076923	4
GAGTGTT	120	1.0363692E-7	16.958332	7
TAGAGTG	120	1.0363692E-7	16.958332	5
GGTTTTC	120	1.0363692E-7	16.958332	27
AGAATTT	165	5.2750693E-11	16.818182	17
>>END_MODULE