FastQCFastQC Report
Fri 10 Feb 2017
ERR1631608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170866
Sequences flagged as poor quality0
Sequence length43
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77694.546837872953074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58053.39739913148315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52343.063219130780846No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26701.5626280242997437No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9450.5530649748926059No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA8900.5208760080999145No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.4711294230566643No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.4512307890393642No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7540.4412814720307141No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.4102630131213934No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA6330.37046574508679314No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA6210.3634426977865696No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6100.35700490442803134No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.32774220734376647No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4760.27858087624220146No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.2703873210586073No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4300.2516591949246778No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA4070.23819835426591596No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA3770.22064073601535705No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.20600938747322464No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.20191260988142753No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA3290.1925485468144628No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT3230.189037023164351No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA3180.18611075345592454No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT2920.1708941509721068No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT2910.17030889703042149No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA2860.16738262732199502No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT2730.15977432608008615No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT2660.15567754848828907No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT2610.15275127877986258No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2570.15041026301312138No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2510.14689873936300962No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG2450.14338721571289784No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC2420.14163145388784193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2380.13929043812110073No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2370.13870518417941544No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA2340.13694942235435956No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC2290.13402315264593306No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT2240.13109688293750657No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.1252443435206536No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2120.12407383563728303No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA2100.12290332775391243No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2080.12173281987054184No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC2040.11939180410380063No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT2030.11880655016211533No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA2030.11880655016211533No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA2000.11705078833705944No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT1940.11353926468694767No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.1088572331534653No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA1850.10827197921178No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1850.10827197921178No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT1830.1071014713284094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1800.10534570950335351No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.10476045556166821No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1730.10124893191155641No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCTG200.001837554337.036
ATATGCT200.001837554337.035
GCAGCGT303.5867776E-430.8333341
CAGCGTC303.5867776E-430.8333342
AGCGTCA303.5867776E-430.8333343
GAACCAT250.00548341829.5999986
GGCACTG405.911028E-527.7534
AAGCAGG405.911028E-527.7524
AGCAGGG358.838395E-426.42857225
TGAACCA358.838395E-426.4285725
TATTGAC509.032081E-625.89999830
ATTGACT509.032081E-625.89999831
ACCGACC451.3167817E-424.66666632
GACCTGT451.3167817E-424.66666635
CGTTGAA451.3167817E-424.6666662
ATACACT451.3167817E-424.6666664
ACCATTC400.00192450223.1258
GCGTCAG400.00192450223.1254
TTGAACC400.00192450223.1254
CCGACCT502.6890772E-422.19999933
Produced by FastQC (version 0.11.5)