##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631608.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	170866
Sequences flagged as poor quality	0
Sequence length	43
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.862319010218535	34.0	33.0	34.0	31.0	34.0
2	32.88864373251554	34.0	33.0	34.0	31.0	34.0
3	32.87683916051174	34.0	33.0	34.0	31.0	34.0
4	36.223215853358774	37.0	35.0	37.0	35.0	37.0
5	36.25186403380427	37.0	37.0	37.0	35.0	37.0
6	36.40218650872614	37.0	37.0	37.0	35.0	37.0
7	36.3912364074772	37.0	37.0	37.0	35.0	37.0
8	36.47036274039306	37.0	37.0	37.0	35.0	37.0
9	38.25193426427727	39.0	39.0	39.0	37.0	39.0
10	38.230139407488906	39.0	39.0	39.0	37.0	39.0
11	38.29888333547927	39.0	39.0	39.0	37.0	39.0
12	38.314837357929605	39.0	39.0	39.0	37.0	39.0
13	38.33949410649281	39.0	39.0	39.0	37.0	39.0
14	39.6325541652523	41.0	39.0	41.0	37.0	41.0
15	39.52595601231374	41.0	39.0	41.0	37.0	41.0
16	39.40153687685086	41.0	39.0	41.0	37.0	41.0
17	39.546264324090224	41.0	39.0	41.0	37.0	41.0
18	39.70152634227991	41.0	40.0	41.0	37.0	41.0
19	39.76504395257102	41.0	40.0	41.0	37.0	41.0
20	39.746462139922514	41.0	40.0	41.0	38.0	41.0
21	39.68155747778961	41.0	40.0	41.0	37.0	41.0
22	39.531012606369906	41.0	40.0	41.0	35.0	41.0
23	39.389913733568996	41.0	39.0	41.0	35.0	41.0
24	39.28909203703487	41.0	39.0	41.0	35.0	41.0
25	39.2430208467454	41.0	39.0	41.0	35.0	41.0
26	38.92419205693351	41.0	38.0	41.0	35.0	41.0
27	38.69733007151803	41.0	37.0	41.0	35.0	41.0
28	38.4454075123196	40.0	37.0	41.0	35.0	41.0
29	38.137815598188055	40.0	35.0	41.0	35.0	41.0
30	37.73589245373567	39.0	35.0	41.0	35.0	41.0
31	37.26809897814662	39.0	35.0	41.0	35.0	41.0
32	36.869669799726104	38.0	35.0	41.0	34.0	41.0
33	36.478380719394146	38.0	35.0	41.0	33.0	41.0
34	36.156286212587645	38.0	35.0	41.0	33.0	41.0
35	35.769965938220594	38.0	35.0	41.0	32.0	41.0
36	35.366140718457736	37.0	35.0	41.0	30.0	41.0
37	34.92043472662788	37.0	35.0	41.0	23.0	41.0
38	34.477731087518876	36.0	35.0	41.0	18.0	41.0
39	33.93014408952045	35.0	35.0	41.0	15.0	41.0
40	33.336017698079196	35.0	35.0	41.0	10.0	41.0
41	32.6837053597556	35.0	34.0	41.0	8.0	41.0
42	31.9900331253731	35.0	33.0	41.0	7.0	41.0
43	30.99711469806749	35.0	30.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	3.0
13	0.0
14	4.0
15	3.0
16	4.0
17	4.0
18	11.0
19	26.0
20	38.0
21	85.0
22	133.0
23	227.0
24	352.0
25	636.0
26	949.0
27	1411.0
28	2193.0
29	3034.0
30	3787.0
31	4171.0
32	4531.0
33	5465.0
34	6791.0
35	8358.0
36	11545.0
37	34434.0
38	26746.0
39	55918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.19410532229935	15.876183676097058	17.856097760818418	17.073613240785175
2	16.548640455093466	27.310875188744394	33.64332283777931	22.49716151838283
3	20.834455070054897	30.153453583509886	30.76387344468765	18.24821790174757
4	12.210738239322042	21.019980569569135	29.711586857537487	37.05769433357134
5	9.815879109945804	44.31542846441071	29.197733896737795	16.67095852890569
6	23.078318682476326	33.1581473201222	22.355530064494985	21.40800393290649
7	23.435908840846043	36.88036238924069	21.34538176114616	18.3383470087671
8	34.531153067315906	28.827268151651005	19.663361932742617	16.978216848290472
9	31.098638699331637	13.069891025716057	20.812215420270856	35.01925485468145
10	20.959114159633867	33.01593061229268	22.70960869921459	23.31534652885887
11	26.559994381562156	30.155209345334942	17.14501422167078	26.139782051432114
12	21.549050132852646	38.264487961326424	26.06721056266314	14.119251343157796
13	32.083621083188	22.44097713998104	29.412522093336296	16.062879683494668
14	22.3110507649269	25.484882890686265	32.93048353680662	19.27358280758021
15	26.96323434738333	28.449194105322302	28.062926503810004	16.52464504348437
16	15.208994182575818	30.70768906628586	33.4648203855653	20.618496365573023
17	12.58061873046715	36.62285065489916	31.997588753760258	18.798941860873434
18	14.911685180199688	26.734985310126063	38.86847002914565	19.484859480528602
19	15.364086477122424	32.94277386958201	40.87530579518453	10.817833858111033
20	18.592347219458524	27.063312771411514	42.200905973101726	12.143434036028232
21	22.275350274484097	26.43709105380825	38.55243290063559	12.735125771072067
22	19.596057729448805	27.900225908021493	35.572905083515735	16.930811279013962
23	15.924174499315253	30.727002446361478	36.54676764248007	16.8020554118432
24	13.699624266969437	31.753537860077486	40.46738379782988	14.079454075123197
25	13.194550115295026	30.961104023035595	38.5717462807112	17.27259958095818
26	17.1245303337118	29.35633771493451	37.890510692589515	15.628621258764177
27	15.319607177554339	30.67667060737654	36.97751454356045	17.02620767150867
28	13.248393477930072	31.213933725843646	40.83199700349982	14.705675792726463
29	13.610080413891588	31.8366439197968	41.22762866807908	13.325646998232532
30	12.673088853253429	32.839769175845404	42.14589210258331	12.341249868317863
31	13.001416314538877	34.078751770393175	38.934603724556084	13.985228190511863
32	11.698641040347407	33.98979317125701	41.93051865204312	12.381047136352464
33	12.660798520478037	32.84503646132057	41.24401577844627	13.25014923975513
34	12.823499116266548	33.40980651504688	36.9078693244999	16.858825044186673
35	11.78291760795009	34.4837474980394	37.105685156789534	16.62764973722098
36	10.00433087916847	33.97574707665656	37.87002680463053	18.14989523954444
37	10.892161108705068	33.835286130652094	38.51673241019278	16.75582035045006
38	11.646553439537415	30.984514180703005	40.0565355307668	17.31239684899278
39	11.998876312431964	31.53874966347898	40.295904392916086	16.166469631172966
40	11.314129200660167	31.445109032809338	38.51790291807615	18.722858848454344
41	9.076703381597275	32.77422073437665	39.560825442159356	18.588250441866727
42	9.16449147285007	32.00987908653565	38.29901794388586	20.52661149672843
43	8.423559982676483	34.42171058022076	38.33822995797876	18.81649947912399
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33.0
1	93.0
2	153.0
3	671.5
4	1190.0
5	1190.0
6	1574.0
7	1958.0
8	1994.5
9	2031.0
10	3153.0
11	4275.0
12	4275.0
13	6937.5
14	9600.0
15	13337.0
16	17074.0
17	14705.5
18	12337.0
19	12337.0
20	12938.5
21	13540.0
22	9525.5
23	5511.0
24	4682.5
25	3854.0
26	3854.0
27	3420.0
28	2986.0
29	2957.0
30	2928.0
31	2854.0
32	2780.0
33	2780.0
34	2681.0
35	2582.0
36	2475.0
37	2368.0
38	2989.0
39	3610.0
40	3610.0
41	4521.0
42	5432.0
43	7106.5
44	8781.0
45	11186.0
46	13591.0
47	13591.0
48	15588.0
49	17585.0
50	15526.5
51	13468.0
52	9761.5
53	6055.0
54	6055.0
55	4573.0
56	3091.0
57	2750.5
58	2410.0
59	2287.5
60	2165.0
61	2165.0
62	2071.0
63	1977.0
64	1912.0
65	1847.0
66	1770.0
67	1693.0
68	1693.0
69	1520.5
70	1348.0
71	1194.0
72	1040.0
73	875.5
74	711.0
75	711.0
76	576.0
77	441.0
78	332.0
79	223.0
80	170.5
81	118.0
82	118.0
83	83.0
84	48.0
85	33.5
86	19.0
87	14.5
88	10.0
89	10.0
90	6.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	170866.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.75240246743062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.14234834558823	34.29002844334156
2	7.156192555147059	5.832640782835672
3	2.639590992647059	3.2270902344527292
4	1.4260684742647058	2.3246286563740006
5	0.8832146139705883	1.7996558706822892
6	0.6002987132352942	1.4678168857467255
7	0.40067784926470584	1.1430009481113854
8	0.2972771139705882	0.9691805274308524
9	0.2585018382352941	0.9481113855301815
>10	1.835363051470588	14.691044444184332
>50	0.19818474264705882	5.65121206091323
>100	0.14217601102941177	10.819004365994404
>500	0.014361213235294119	4.2665012348858165
>1k	0.0014361213235294116	1.5626280242997437
>5k	0.004308363970588236	11.00745613521707
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7769	4.546837872953074	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5805	3.39739913148315	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5234	3.063219130780846	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2670	1.5626280242997437	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	945	0.5530649748926059	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	890	0.5208760080999145	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	805	0.4711294230566643	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	771	0.4512307890393642	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	754	0.4412814720307141	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	701	0.4102630131213934	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	633	0.37046574508679314	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	621	0.3634426977865696	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	610	0.35700490442803134	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	560	0.32774220734376647	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	476	0.27858087624220146	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	462	0.2703873210586073	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	430	0.2516591949246778	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	407	0.23819835426591596	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	377	0.22064073601535705	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	352	0.20600938747322464	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	345	0.20191260988142753	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	329	0.1925485468144628	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	323	0.189037023164351	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	318	0.18611075345592454	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	292	0.1708941509721068	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	291	0.17030889703042149	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	286	0.16738262732199502	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	273	0.15977432608008615	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	266	0.15567754848828907	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	261	0.15275127877986258	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	257	0.15041026301312138	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	251	0.14689873936300962	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	245	0.14338721571289784	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	242	0.14163145388784193	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	238	0.13929043812110073	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	237	0.13870518417941544	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	234	0.13694942235435956	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	229	0.13402315264593306	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	224	0.13109688293750657	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	214	0.1252443435206536	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	212	0.12407383563728303	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	210	0.12290332775391243	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	208	0.12173281987054184	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	204	0.11939180410380063	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	203	0.11880655016211533	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	203	0.11880655016211533	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	200	0.11705078833705944	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	194	0.11353926468694767	No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	186	0.1088572331534653	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	185	0.10827197921178	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	185	0.10827197921178	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	183	0.1071014713284094	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	180	0.10534570950335351	No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	179	0.10476045556166821	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	173	0.10124893191155641	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.852539416852972E-4
6	5.852539416852972E-4	0.0	0.0	0.0	5.852539416852972E-4
7	5.852539416852972E-4	0.0	0.0	0.0011705078833705944	5.852539416852972E-4
8	5.852539416852972E-4	0.0	0.0	0.0017557618250558917	5.852539416852972E-4
9	5.852539416852972E-4	0.0	0.0	0.004682031533482378	5.852539416852972E-4
10	5.852539416852972E-4	0.0	0.0	0.019898634017300108	5.852539416852972E-4
11	5.852539416852972E-4	0.0	0.0	0.0415530298596561	5.852539416852972E-4
12	5.852539416852972E-4	0.0	0.0	0.06847471117717978	5.852539416852972E-4
13	5.852539416852972E-4	0.0	0.0	0.07783877424414454	5.852539416852972E-4
14	5.852539416852972E-4	0.0	0.0	0.10066367796987112	5.852539416852972E-4
15	5.852539416852972E-4	0.0	0.0	0.14338721571289784	5.852539416852972E-4
16	5.852539416852972E-4	0.0	0.0	0.20542413353153932	5.852539416852972E-4
17	5.852539416852972E-4	0.0	0.0	0.28853019325085155	5.852539416852972E-4
18	5.852539416852972E-4	0.0	0.0	0.32306017581028407	5.852539416852972E-4
19	5.852539416852972E-4	0.0	0.0	0.37046574508679314	5.852539416852972E-4
20	5.852539416852972E-4	0.0	0.0	0.43718469443891705	5.852539416852972E-4
21	5.852539416852972E-4	0.0	0.0	0.5290695632835087	5.852539416852972E-4
22	5.852539416852972E-4	0.0	0.0	0.7567333465990893	5.852539416852972E-4
23	5.852539416852972E-4	0.0	0.0	0.9662542577224258	5.852539416852972E-4
24	5.852539416852972E-4	0.0	0.0	1.2606369903901302	5.852539416852972E-4
25	5.852539416852972E-4	0.0	0.0	1.352521859234722	5.852539416852972E-4
26	5.852539416852972E-4	0.0	0.0	1.4877155197640257	5.852539416852972E-4
27	5.852539416852972E-4	0.0	0.0	1.6340290051853499	5.852539416852972E-4
28	5.852539416852972E-4	0.0	0.0	1.9570891809956341	5.852539416852972E-4
29	5.852539416852972E-4	0.0	0.0	2.5540482015146373	5.852539416852972E-4
30	5.852539416852972E-4	0.0	0.0	3.684173562908946	5.852539416852972E-4
31	5.852539416852972E-4	0.0	0.0	5.376727962262826	5.852539416852972E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCTG	20	0.0018375543	37.0	36
ATATGCT	20	0.0018375543	37.0	35
GCAGCGT	30	3.5867776E-4	30.833334	1
CAGCGTC	30	3.5867776E-4	30.833334	2
AGCGTCA	30	3.5867776E-4	30.833334	3
GAACCAT	25	0.005483418	29.599998	6
GGCACTG	40	5.911028E-5	27.75	34
AAGCAGG	40	5.911028E-5	27.75	24
AGCAGGG	35	8.838395E-4	26.428572	25
TGAACCA	35	8.838395E-4	26.428572	5
TATTGAC	50	9.032081E-6	25.899998	30
ATTGACT	50	9.032081E-6	25.899998	31
ACCGACC	45	1.3167817E-4	24.666666	32
GACCTGT	45	1.3167817E-4	24.666666	35
CGTTGAA	45	1.3167817E-4	24.666666	2
ATACACT	45	1.3167817E-4	24.666666	4
ACCATTC	40	0.001924502	23.125	8
GCGTCAG	40	0.001924502	23.125	4
TTGAACC	40	0.001924502	23.125	4
CCGACCT	50	2.6890772E-4	22.199999	33
>>END_MODULE