FastQCFastQC Report
Fri 10 Feb 2017
ERR1631607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24509
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT750.30601003712921787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT540.22032722673303684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT430.17544575462075157No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA390.15912521930719328No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA360.14688481782202456No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT340.13872455016524543No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT290.11832388102329756No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT290.11832388102329756No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT260.10608347953812884No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGG200.001807004337.08
ACATCTC200.001807004337.08
CCGAGCC400.001878057523.12514
GGTATCA1302.7284841E-1119.9230771
GTATCAA1354.5474735E-1119.1851842
AACGCAG1556.1263563E-915.51612957
CAACGCA1701.2314558E-915.2352936
TCAACGC1751.8571882E-914.85
CATGGGA750.003954021714.818
ATCAACG1751.8571882E-914.84
TATCAAC1802.764864E-914.3888883
GAGTACA1551.05432264E-714.32258113
AGTACAT1601.554672E-713.87514
ACATGGG1652.2635322E-713.45454517
ACGCAGA1804.39868E-813.3611118
TCTTATA1958.536517E-913.28205237
CGCAGAG1856.299342E-813.0000019
TACATGG1754.6326568E-712.68571516
CAGAGTA1951.2535565E-712.33333411
AGAGTAC1951.2535565E-712.33333412