##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631607.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24509
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96927659227222	34.0	33.0	34.0	31.0	34.0
2	33.13191072667183	34.0	33.0	34.0	31.0	34.0
3	33.166959076257704	34.0	33.0	34.0	31.0	34.0
4	36.456036557999106	37.0	37.0	37.0	35.0	37.0
5	36.47080664245787	37.0	37.0	37.0	35.0	37.0
6	36.535272756946426	37.0	37.0	37.0	35.0	37.0
7	36.50813986698764	37.0	37.0	37.0	35.0	37.0
8	36.51454567709821	37.0	37.0	37.0	35.0	37.0
9	38.35901097556	39.0	39.0	39.0	37.0	39.0
10	38.320412909543435	39.0	39.0	39.0	37.0	39.0
11	38.38920396589008	39.0	39.0	39.0	37.0	39.0
12	38.339711942551716	39.0	39.0	39.0	37.0	39.0
13	38.35607327920356	39.0	39.0	39.0	37.0	39.0
14	39.85756252805092	41.0	40.0	41.0	38.0	41.0
15	39.863152311395815	41.0	40.0	41.0	38.0	41.0
16	39.79587090456567	41.0	40.0	41.0	38.0	41.0
17	39.75857848137419	41.0	40.0	41.0	38.0	41.0
18	39.77665347423395	41.0	40.0	41.0	38.0	41.0
19	39.833204129095435	41.0	40.0	41.0	38.0	41.0
20	39.817373209841286	41.0	40.0	41.0	38.0	41.0
21	39.796278917948506	41.0	40.0	41.0	38.0	41.0
22	39.711412134318	41.0	40.0	41.0	37.0	41.0
23	39.66563303276347	41.0	40.0	41.0	37.0	41.0
24	39.619649924517525	41.0	40.0	41.0	37.0	41.0
25	39.59153780243992	41.0	40.0	41.0	37.0	41.0
26	39.489126443347345	41.0	39.0	41.0	37.0	41.0
27	39.45016116528622	41.0	39.0	41.0	36.0	41.0
28	39.35366600024481	41.0	39.0	41.0	36.0	41.0
29	39.29409604635032	41.0	39.0	41.0	36.0	41.0
30	39.19250071402342	41.0	39.0	41.0	35.0	41.0
31	39.106899506303805	41.0	39.0	41.0	35.0	41.0
32	39.02701048594394	40.0	39.0	41.0	35.0	41.0
33	38.94010363539924	40.0	39.0	41.0	35.0	41.0
34	38.847362193479945	40.0	38.0	41.0	35.0	41.0
35	38.77583744746828	40.0	38.0	41.0	35.0	41.0
36	38.70467991350116	40.0	38.0	41.0	35.0	41.0
37	38.65665673834102	40.0	38.0	41.0	35.0	41.0
38	38.5682810396181	40.0	38.0	41.0	35.0	41.0
39	38.429026072055166	40.0	38.0	41.0	35.0	41.0
40	38.3286139785385	40.0	37.0	41.0	35.0	41.0
41	38.23889183565221	40.0	37.0	41.0	35.0	41.0
42	38.12309763760251	40.0	37.0	41.0	34.0	41.0
43	37.24289036680403	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	2.0
22	4.0
23	5.0
24	21.0
25	23.0
26	26.0
27	61.0
28	57.0
29	108.0
30	156.0
31	170.0
32	240.0
33	328.0
34	432.0
35	738.0
36	1154.0
37	2265.0
38	5122.0
39	13595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.139254967562934	16.691827491941737	14.231506793422824	25.937410747072505
2	21.767513974458364	18.495246644089928	32.29833938553185	27.43889999591987
3	20.800522257130034	20.784201721816476	28.61805867232445	29.79721734872904
4	15.50858868170876	16.74894936553919	31.221184054836996	36.52127789791505
5	17.087600473295524	33.57134113998939	31.35582847117385	17.98522991554123
6	34.489371251377044	31.13958137826921	15.949243135174834	18.421804235178914
7	30.494920233383656	29.20559794361255	18.352441960096293	21.947039862907502
8	29.821698151699376	30.34803541556163	18.238198212901384	21.59206821983761
9	27.60210534905545	13.472601901342365	17.956668978742503	40.968623770859686
10	21.926639193765553	22.979313721490065	26.753437512750416	28.340609571993962
11	37.12105757068832	21.277897915051614	17.360969439797625	24.240075074462442
12	24.223754539148885	25.998612754498346	23.628055000204007	26.14957770614876
13	32.71451303602758	17.173283283691703	23.179240279081153	26.93296340119956
14	24.966338895915786	19.172548859602596	22.049043208617242	33.812069035864376
15	30.229711534538335	22.901791178750663	19.384715818678853	27.483781468032152
16	26.53311028601738	23.78310008568281	22.26529030152189	27.41849932677792
17	25.243787996246276	25.468195356807705	22.15104655432698	27.136970092619038
18	25.87620873964666	21.718552368517688	24.329838018687013	28.075400873148638
19	26.524950018360606	24.42776123056836	23.33836549838835	25.708923252682688
20	27.455220531233426	21.069811089803743	25.02346076951324	26.451507609449592
21	30.92333428536456	21.363580725447797	21.996001468848178	25.717083520339468
22	29.19335754212738	22.020482271818516	21.52270594475499	27.26345424129911
23	27.60210534905545	22.224488963237995	22.326492308947735	27.846913378758824
24	26.586152025786447	23.248602554163778	22.987473989146846	27.177771430902936
25	26.337263862254684	23.00787465828879	23.060916398057856	27.59394508139867
26	28.61805867232445	22.122485617528255	22.95075278469134	26.308702925455957
27	28.38549104410625	22.089844546901137	21.755273572973195	27.76939083601942
28	25.684442449712353	22.044963074788853	24.91737728997511	27.353217185523686
29	25.733404055653025	22.375453914888407	25.741564323309806	26.14957770614876
30	25.65180137908523	22.306091639805786	26.09245583255131	25.949651148557674
31	25.933330613244117	22.575380472479498	23.885103431392547	27.606185482883838
32	24.66440899261496	22.053123342445634	26.129177037006812	27.1532906279326
33	24.578726182218777	21.163654167856706	26.806479252519484	27.451140397405034
34	25.627320576114897	20.812762658615203	25.929250479415728	27.630666285854176
35	24.872495817862823	20.76788118650292	26.4066261373373	27.952996858296952
36	23.375086702843852	20.310906197723284	27.243053572157166	29.070953527275694
37	23.248602554163778	18.980782569668285	28.785344159288424	28.985270716879512
38	22.689624219674407	17.70370068138235	30.666285854176017	28.94038924476723
39	21.996001468848178	17.96890938022767	32.50234607695132	27.532743073972828
40	21.171814435513483	18.274919417356887	32.384022195928026	28.1692439512016
41	18.968542168183117	19.131747521318697	34.08951813619487	27.810192174303317
42	18.82573748418948	19.976335223795342	32.90627932596189	28.291647966053286
43	17.467052919335753	20.857644130727486	32.9838018687013	28.691501081235465
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	7.0
4	11.0
5	11.0
6	13.0
7	15.0
8	17.0
9	19.0
10	30.5
11	42.0
12	42.0
13	55.5
14	69.0
15	115.0
16	161.0
17	137.5
18	114.0
19	114.0
20	125.5
21	137.0
22	103.0
23	69.0
24	65.5
25	62.0
26	62.0
27	67.0
28	72.0
29	110.0
30	148.0
31	170.5
32	193.0
33	193.0
34	267.0
35	341.0
36	443.5
37	546.0
38	664.5
39	783.0
40	783.0
41	950.0
42	1117.0
43	1303.0
44	1489.0
45	1730.0
46	1971.0
47	1971.0
48	2106.5
49	2242.0
50	2191.5
51	2141.0
52	2086.0
53	2031.0
54	2031.0
55	1832.0
56	1633.0
57	1616.0
58	1599.0
59	1541.5
60	1484.0
61	1484.0
62	1449.5
63	1415.0
64	1339.5
65	1264.0
66	1130.0
67	996.0
68	996.0
69	899.5
70	803.0
71	697.5
72	592.0
73	489.5
74	387.0
75	387.0
76	326.0
77	265.0
78	214.0
79	163.0
80	113.0
81	63.0
82	63.0
83	50.5
84	38.0
85	26.0
86	14.0
87	11.5
88	9.0
89	9.0
90	7.5
91	6.0
92	3.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	24509.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.52144110326819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.56675956422599	72.12044555061406
2	6.252850266024828	10.069770288465461
3	1.7532303014948063	4.235178913868374
4	0.9171522675449708	2.95401689175405
5	0.5016468203699012	2.0196662450528375
6	0.25335697998479856	1.2240401485168715
7	0.19255130478844693	1.085315598351626
8	0.10640993159361539	0.685462483169448
9	0.12161135039270331	0.8813089069321474
>10	0.3242969343805422	4.198457709412868
>50	0.010134279199391943	0.5263372638622547
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	75	0.30601003712921787	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.22032722673303684	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	43	0.17544575462075157	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	39	0.15912521930719328	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	36	0.14688481782202456	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	34	0.13872455016524543	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	29	0.11832388102329756	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	29	0.11832388102329756	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	26	0.10608347953812884	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.004080133828389571	0.0
6	0.0	0.0	0.0	0.004080133828389571	0.0
7	0.0	0.0	0.0	0.004080133828389571	0.0
8	0.0	0.0	0.0	0.004080133828389571	0.0
9	0.0	0.0	0.0	0.004080133828389571	0.0
10	0.0	0.0	0.0	0.004080133828389571	0.0
11	0.0	0.0	0.0	0.008160267656779142	0.0
12	0.0	0.0	0.0	0.008160267656779142	0.0
13	0.0	0.0	0.0	0.008160267656779142	0.0
14	0.0	0.0	0.0	0.008160267656779142	0.0
15	0.0	0.0	0.0	0.008160267656779142	0.0
16	0.0	0.0	0.0	0.012240401485168714	0.0
17	0.0	0.0	0.0	0.012240401485168714	0.0
18	0.0	0.0	0.0	0.012240401485168714	0.0
19	0.0	0.0	0.0	0.012240401485168714	0.0
20	0.0	0.0	0.0	0.012240401485168714	0.0
21	0.0	0.0	0.0	0.016320535313558284	0.0
22	0.0	0.0	0.0	0.028560936798727	0.0
23	0.0	0.0	0.0	0.04080133828389571	0.0
24	0.0	0.0	0.0	0.10608347953812886	0.0
25	0.0	0.0	0.0	0.1264841486800767	0.0
26	0.0	0.0	0.0	0.16320535313558285	0.0
27	0.0	0.0	0.0	0.3876127136970093	0.0
28	0.0	0.0	0.0	1.0567546615528989	0.0
29	0.0	0.0	0.0	1.9135827655147088	0.0
30	0.0	0.0	0.0	3.0192990330082825	0.0
31	0.0	0.0	0.0	5.087926884001795	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGGGG	20	0.0018070043	37.0	8
ACATCTC	20	0.0018070043	37.0	8
CCGAGCC	40	0.0018780575	23.125	14
GGTATCA	130	2.7284841E-11	19.923077	1
GTATCAA	135	4.5474735E-11	19.185184	2
AACGCAG	155	6.1263563E-9	15.5161295	7
CAACGCA	170	1.2314558E-9	15.235293	6
TCAACGC	175	1.8571882E-9	14.8	5
CATGGGA	75	0.0039540217	14.8	18
ATCAACG	175	1.8571882E-9	14.8	4
TATCAAC	180	2.764864E-9	14.388888	3
GAGTACA	155	1.05432264E-7	14.322581	13
AGTACAT	160	1.554672E-7	13.875	14
ACATGGG	165	2.2635322E-7	13.454545	17
ACGCAGA	180	4.39868E-8	13.361111	8
TCTTATA	195	8.536517E-9	13.282052	37
CGCAGAG	185	6.299342E-8	13.000001	9
TACATGG	175	4.6326568E-7	12.685715	16
CAGAGTA	195	1.2535565E-7	12.333334	11
AGAGTAC	195	1.2535565E-7	12.333334	12
>>END_MODULE