FastQCFastQC Report
Fri 10 Feb 2017
ERR1631606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254490
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT114984.518055719281701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82083.2252740775668984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79013.104640653856733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32431.2743133325474478No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA16950.6660379582694801No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA11530.45306298872254314No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9790.384690950528508No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA9620.3780109238084011No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.37683209556367636No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9570.37604621006719324No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA8340.3277142520334787No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7210.28331172148218003No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.2785964085032811No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA7090.2785964085032811No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT7020.27584580926559No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.26877283979724154No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC6830.268379897049No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA6660.2616998703288931No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT6610.2597351565876852No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC6420.2522692443710951No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT6370.2503045306298872No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT6360.24991158788164564No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.2389091909308814No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA5380.2114031985539707No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5220.20511611458210538No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT5150.20236551534441433No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.18311132068057684No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.17289480922629574No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC4140.16267829777201462No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG3930.1544265000589414No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA3900.15324767181421667No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT3790.14892530158355927No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.14853235883531768No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA3770.14813941608707612No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT3690.14499587410114345No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.1422452748634524No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3320.13045699241620495No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3170.12456285119258124No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA3140.1233840229478565No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT3140.1233840229478565No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA3110.12220519470313175No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT3070.12063342371016544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3040.1194545954654407No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG2900.11395339699005855No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT2720.10688042752171009No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA2680.10530865652874377No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT2650.10412982828401902No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT2630.10334394278753585No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC2620.10295100003929428No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT2590.10177217179456954No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2570.10098628629808637No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.1002004008016032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGGG303.5916752E-430.8333345
GAACTAC250.005488402729.61
TCGCTAC250.005488402729.627
GACGGTA250.005488402729.68
CTACCTT250.005488402729.630
TTCGCTA250.005488402729.626
ACCTTAG250.005488402729.632
ACGGTAT250.005488402729.69
ATGCCCC1900.027.26315937
CAAAGCA358.8503474E-426.4285724
CGGACCA451.3192603E-424.6666669
TTAAGAG400.001927079923.1253
CATATCC502.6940973E-422.234
GGACGGC502.6940973E-422.210
TCAAAGC502.6940973E-422.23
GAGGGCA2100.022.023819
TATTGAC1350.021.92592630
GGGGCAT603.7116282E-521.58333419
CAAGACG705.0762046E-621.1428594
TGATCCG450.003817748320.5555555
Produced by FastQC (version 0.11.5)