##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631606.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254490
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85529883296004	34.0	31.0	34.0	31.0	34.0
2	32.906943298361426	34.0	33.0	34.0	31.0	34.0
3	32.889060473888954	34.0	33.0	34.0	31.0	34.0
4	36.28569688396401	37.0	37.0	37.0	35.0	37.0
5	36.31076270187434	37.0	37.0	37.0	35.0	37.0
6	36.43006012024048	37.0	37.0	37.0	35.0	37.0
7	36.4240677433298	37.0	37.0	37.0	35.0	37.0
8	36.46134229242799	37.0	37.0	37.0	35.0	37.0
9	38.23301112027978	39.0	39.0	39.0	37.0	39.0
10	38.20893551809501	39.0	39.0	39.0	37.0	39.0
11	38.275126724036305	39.0	39.0	39.0	37.0	39.0
12	38.26595544029235	39.0	39.0	39.0	37.0	39.0
13	38.28387362961217	39.0	39.0	39.0	37.0	39.0
14	39.555868599944986	41.0	39.0	41.0	37.0	41.0
15	39.467629376399856	41.0	39.0	41.0	37.0	41.0
16	39.372639396439936	40.0	39.0	41.0	37.0	41.0
17	39.483551416558605	40.0	39.0	41.0	37.0	41.0
18	39.66055640693151	41.0	40.0	41.0	38.0	41.0
19	39.71803607214429	41.0	40.0	41.0	38.0	41.0
20	39.7400212189084	41.0	40.0	41.0	38.0	41.0
21	39.712141930920666	41.0	40.0	41.0	37.0	41.0
22	39.61842901489253	41.0	40.0	41.0	37.0	41.0
23	39.5182561200833	41.0	40.0	41.0	36.0	41.0
24	39.45994341624425	41.0	39.0	41.0	35.0	41.0
25	39.43516837596762	41.0	39.0	41.0	35.0	41.0
26	39.20056583755747	41.0	39.0	41.0	35.0	41.0
27	39.031875515737354	41.0	38.0	41.0	35.0	41.0
28	38.832877519745374	40.0	38.0	41.0	35.0	41.0
29	38.56760187040748	40.0	37.0	41.0	35.0	41.0
30	38.22970254233958	40.0	37.0	41.0	35.0	41.0
31	37.83466147982239	40.0	35.0	41.0	35.0	41.0
32	37.50814177374357	39.0	35.0	41.0	35.0	41.0
33	37.20198043145114	39.0	35.0	41.0	34.0	41.0
34	36.9781445243428	39.0	35.0	41.0	33.0	41.0
35	36.708012102636644	39.0	35.0	41.0	33.0	41.0
36	36.415705921647216	39.0	35.0	41.0	33.0	41.0
37	36.09803528625879	38.0	35.0	41.0	33.0	41.0
38	35.771169790561515	38.0	35.0	41.0	31.0	41.0
39	35.36022240559551	38.0	35.0	41.0	28.0	41.0
40	34.94072851585524	37.0	35.0	41.0	23.0	41.0
41	34.438901332075915	37.0	35.0	41.0	18.0	41.0
42	33.882541553695624	37.0	35.0	41.0	13.0	41.0
43	32.89144956579826	35.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	4.0
13	7.0
14	4.0
15	8.0
16	10.0
17	8.0
18	10.0
19	23.0
20	39.0
21	77.0
22	109.0
23	194.0
24	331.0
25	525.0
26	823.0
27	1409.0
28	2127.0
29	3092.0
30	3981.0
31	4528.0
32	4967.0
33	6292.0
34	8434.0
35	11329.0
36	17892.0
37	51456.0
38	50663.0
39	86145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.89936736217533	14.090926951943104	17.058823529411764	19.950882156469802
2	17.85099610986679	24.54241816967268	34.44536131085701	23.16122440960352
3	19.618452591457427	27.93037054501159	30.916735431647606	21.534441431883376
4	11.4165586074109	19.250658179103304	30.941097882038587	38.391685331447206
5	11.157216393571456	43.918425085465046	28.5229282093599	16.401430311603598
6	25.094109788203863	32.93921175684702	22.12974969546937	19.836928759479743
7	27.339777594404495	34.827301662147825	18.5343235490589	19.298597194388776
8	37.21403591496719	27.305591575307474	17.208141773743566	18.27223073598177
9	32.68183425674879	11.70969389759912	18.098157098510747	37.510314747141344
10	21.094738496601046	30.842862194978192	21.482572989115486	26.579826319305276
11	30.873904672089274	28.998388934732212	15.339699005854849	24.788007387323667
12	24.332193799363434	34.36559393296397	24.61275492160792	16.68945734606468
13	34.79075798656136	19.7457660418877	26.51931313607607	18.94416283547487
14	23.45435969979174	23.334512161578058	29.21529333176156	23.99583480686864
15	29.894298400723013	26.497701284922787	24.584463043734527	19.02353727061967
16	19.29623953789933	27.548823136469014	29.91748202286927	23.23745530276239
17	15.900821250343824	32.933710558371644	29.628669102911704	21.536799088372824
18	19.09544579354788	22.990687256866675	35.49373256316555	22.4201343864199
19	18.37989704899996	30.122205194703135	37.30401980431451	14.193877951982396
20	21.464890565444612	23.59071083343157	38.79563047663955	16.148768124484263
21	25.07249793705057	22.55216314982907	36.24150261306928	16.133836300051083
22	22.78046288655743	23.924319226688674	32.39341427953947	20.90180360721443
23	18.514283468898583	27.281622067664742	33.41192188298165	20.79217258045503
24	16.972376124798615	27.536248968525285	37.67652952964753	17.81484537702857
25	16.670596094149083	26.823057880466816	34.67994813155723	21.82639789382687
26	20.60159534755786	25.74325120829895	34.49330032614248	19.161853118000707
27	18.827851781995363	26.20535188023105	33.457896184525914	21.508900153247673
28	16.299265197060787	27.28397972415419	37.353530590592946	19.06322448819207
29	16.761758811741128	27.90757986561358	38.42587135054423	16.904789972101064
30	15.626547212071202	29.223545129474637	40.08959094659908	15.060316711855082
31	16.489056544461473	30.838539824747535	35.359346143266926	17.313057487524066
32	15.490196078431373	29.969350465637156	38.85417894612755	15.686274509803921
33	16.579433376557034	28.314275610043616	38.6392392628394	16.467051750559943
34	17.515423002868484	29.856968839640064	33.68344532201658	18.94416283547487
35	15.834413925890997	31.159966992809146	34.361664505481556	18.643954575818302
36	13.437856104365595	30.756414790365046	35.67841565483909	20.12731345043027
37	14.874061849188575	30.867224645369166	35.78136665487838	18.477346850563872
38	15.362489685252859	27.254116075287833	37.45726747612873	19.926126763330583
39	16.366851349758342	27.422295571535226	37.45019450666038	18.76065857204605
40	15.06188848284805	27.500491178435304	35.3978545325946	22.03976580612205
41	12.13485795119651	28.96184525914574	38.03646508703682	20.866831702620928
42	12.909348107980668	28.007387323666944	36.64033950253448	22.44292506581791
43	11.556053283036661	30.418484026877284	37.95394710990609	20.07151558017997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16.0
1	48.0
2	80.0
3	465.5
4	851.0
5	851.0
6	1259.0
7	1667.0
8	1751.0
9	1835.0
10	2770.0
11	3705.0
12	3705.0
13	6523.0
14	9341.0
15	15232.0
16	21123.0
17	18127.0
18	15131.0
19	15131.0
20	15954.5
21	16778.0
22	11316.5
23	5855.0
24	5269.0
25	4683.0
26	4683.0
27	4287.0
28	3891.0
29	3828.5
30	3766.0
31	3672.5
32	3579.0
33	3579.0
34	3476.0
35	3373.0
36	3378.0
37	3383.0
38	3889.5
39	4396.0
40	4396.0
41	5558.5
42	6721.0
43	8662.0
44	10603.0
45	13685.0
46	16767.0
47	16767.0
48	21984.0
49	27201.0
50	24946.0
51	22691.0
52	17977.5
53	13264.0
54	13264.0
55	10629.5
56	7995.0
57	7534.0
58	7073.0
59	7047.5
60	7022.0
61	7022.0
62	6967.5
63	6913.0
64	6663.5
65	6414.0
66	5946.0
67	5478.0
68	5478.0
69	4987.0
70	4496.0
71	3908.5
72	3321.0
73	2756.0
74	2191.0
75	2191.0
76	1777.0
77	1363.0
78	1045.5
79	728.0
80	563.5
81	399.0
82	399.0
83	302.0
84	205.0
85	154.5
86	104.0
87	80.5
88	57.0
89	57.0
90	40.5
91	24.0
92	14.5
93	5.0
94	2.5
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	254490.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.427973648584185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.37692749646028	34.111882013753956
2	7.201373531323298	5.8227387871589995
3	2.4869856207701027	3.016313674227045
4	1.3395126243122308	2.1661512431056287
5	0.8686612963018375	1.755910799821783
6	0.574774796079514	1.3942188185863764
7	0.4398276092997258	1.244693729908237
8	0.2888867677493534	0.9343285307196416
9	0.26389656273362383	0.9601922955732203
>10	1.8192869096508142	14.312765374735203
>50	0.18792634011769382	5.290756537425267
>100	0.12595063220653946	9.58333337370171
>500	0.019992163842307852	5.788634023990187
>1k	0.002998824576346178	2.461503060606271
>5k	0.001999216384230785	6.509990609638224
>10k+	9.996081921153925E-4	4.646587127048251
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11498	4.518055719281701	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8208	3.2252740775668984	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7901	3.104640653856733	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3243	1.2743133325474478	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1695	0.6660379582694801	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1153	0.45306298872254314	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	979	0.384690950528508	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	962	0.3780109238084011	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	959	0.37683209556367636	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	957	0.37604621006719324	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	834	0.3277142520334787	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	721	0.28331172148218003	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	709	0.2785964085032811	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	709	0.2785964085032811	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	702	0.27584580926559	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	684	0.26877283979724154	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	683	0.268379897049	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	666	0.2616998703288931	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	661	0.2597351565876852	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	642	0.2522692443710951	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	637	0.2503045306298872	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	636	0.24991158788164564	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.2389091909308814	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	538	0.2114031985539707	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	522	0.20511611458210538	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	515	0.20236551534441433	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	466	0.18311132068057684	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	440	0.17289480922629574	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	414	0.16267829777201462	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	393	0.1544265000589414	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	390	0.15324767181421667	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	379	0.14892530158355927	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	378	0.14853235883531768	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	377	0.14813941608707612	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	369	0.14499587410114345	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	362	0.1422452748634524	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	332	0.13045699241620495	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	317	0.12456285119258124	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	314	0.1233840229478565	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	314	0.1233840229478565	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	311	0.12220519470313175	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	307	0.12063342371016544	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	304	0.1194545954654407	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	290	0.11395339699005855	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	272	0.10688042752171009	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA	268	0.10530865652874377	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	265	0.10412982828401902	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	263	0.10334394278753585	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	262	0.10295100003929428	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	259	0.10177217179456954	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	257	0.10098628629808637	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	255	0.1002004008016032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0011788282447247435	0.0
11	0.0	0.0	0.0	0.007465912216590043	0.0
12	0.0	0.0	0.0	0.013752996188455342	0.0
13	0.0	0.0	0.0	0.016896538174387993	0.0
14	0.0	0.0	0.0	0.021611851153286968	0.0
15	0.0	0.0	0.0	0.02947070611811859	0.0
16	0.0	0.0	0.0	0.0522613855161303	0.0
17	0.0	0.0	0.0	0.07662383590710833	0.0
18	0.0	0.0	0.0	0.08448269087193996	0.0
19	0.0	0.0	0.0	0.09744980156391214	3.929427482415812E-4
20	0.0	0.0	0.0	0.11080985500412589	3.929427482415812E-4
21	0.0	0.0	0.0	0.1355652481433455	3.929427482415812E-4
22	0.0	0.0	0.0	0.19097017564540847	3.929427482415812E-4
23	0.0	0.0	0.0	0.26012809933592673	3.929427482415812E-4
24	0.0	0.0	0.0	0.34814727494204095	3.929427482415812E-4
25	0.0	0.0	0.0	0.37526032457071007	3.929427482415812E-4
26	0.0	0.0	0.0	0.4125898856536603	3.929427482415812E-4
27	0.0	0.0	0.0	0.47978309560297067	3.929427482415812E-4
28	0.0	0.0	0.0	0.6487484773468506	3.929427482415812E-4
29	0.0	0.0	0.0	1.0157570042044874	3.929427482415812E-4
30	0.0	0.0	0.0	1.619317065503556	3.929427482415812E-4
31	0.0	0.0	0.0	2.7168061613422925	3.929427482415812E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGAGGG	30	3.5916752E-4	30.833334	5
GAACTAC	25	0.0054884027	29.6	1
TCGCTAC	25	0.0054884027	29.6	27
GACGGTA	25	0.0054884027	29.6	8
CTACCTT	25	0.0054884027	29.6	30
TTCGCTA	25	0.0054884027	29.6	26
ACCTTAG	25	0.0054884027	29.6	32
ACGGTAT	25	0.0054884027	29.6	9
ATGCCCC	190	0.0	27.263159	37
CAAAGCA	35	8.8503474E-4	26.428572	4
CGGACCA	45	1.3192603E-4	24.666666	9
TTAAGAG	40	0.0019270799	23.125	3
CATATCC	50	2.6940973E-4	22.2	34
GGACGGC	50	2.6940973E-4	22.2	10
TCAAAGC	50	2.6940973E-4	22.2	3
GAGGGCA	210	0.0	22.02381	9
TATTGAC	135	0.0	21.925926	30
GGGGCAT	60	3.7116282E-5	21.583334	19
CAAGACG	70	5.0762046E-6	21.142859	4
TGATCCG	45	0.0038177483	20.555555	5
>>END_MODULE