FastQCFastQC Report
Fri 10 Feb 2017
ERR1631605.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631605.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009652
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.21581693494392126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21220.2101714254020197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16860.16698822960782528No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT16590.16431404087745083No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16490.1633236006069418No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT14750.1460899399000844No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC13210.13083715973424506No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC12790.12667731059810705No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12790.12667731059810705No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12490.12370598978657993No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG11430.11320732291918403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.020.7945751
CTCTATA450.003825490620.5555552
CTAACAC555.1424996E-420.1818183
CGAGCTA801.6163536E-518.522
ACACTTA852.7225838E-517.4117646
CACTTAC852.7225838E-517.4117647
AAGACGG4300.016.779075
ATACTGA1005.8763653E-616.6500026
CAAGACG4550.016.2637354
TCGTTTA2850.016.2280730
ATATAGC700.002592200415.8571436
CGAAACC1059.340767E-615.85714212
GTATCAA16800.015.8571422
AGACGGA4450.015.7977526
GACGGAC4500.015.6222227
ATCTTAC855.3633476E-415.2352941
GTTCAAA5000.015.1700011
AGACCGT1101.4514417E-515.1363646
ACGGACC4700.014.9574468
GTACTAT1001.09315435E-414.81
Produced by FastQC (version 0.11.5)