##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631605.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1009652
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05766937519066	34.0	33.0	34.0	31.0	34.0
2	33.195567383613366	34.0	33.0	34.0	31.0	34.0
3	33.278083933870285	34.0	34.0	34.0	31.0	34.0
4	36.48816522920769	37.0	37.0	37.0	35.0	37.0
5	36.4797653052735	37.0	37.0	37.0	35.0	37.0
6	36.53444949348884	37.0	37.0	37.0	35.0	37.0
7	36.54588808817295	37.0	37.0	37.0	35.0	37.0
8	36.549198139557	37.0	37.0	37.0	35.0	37.0
9	38.39174190711255	39.0	39.0	39.0	37.0	39.0
10	38.36931734894795	39.0	39.0	39.0	37.0	39.0
11	38.41204494221771	39.0	39.0	39.0	37.0	39.0
12	38.377855934520014	39.0	39.0	39.0	37.0	39.0
13	38.403232995131	39.0	39.0	39.0	37.0	39.0
14	39.9499322538855	41.0	40.0	41.0	38.0	41.0
15	39.932556960219955	41.0	40.0	41.0	38.0	41.0
16	39.893271146890214	41.0	40.0	41.0	38.0	41.0
17	39.889537187070395	41.0	40.0	41.0	38.0	41.0
18	39.853695134561214	41.0	40.0	41.0	38.0	41.0
19	39.86239120013629	41.0	40.0	41.0	38.0	41.0
20	39.827420735065154	41.0	40.0	41.0	38.0	41.0
21	39.80471390142346	41.0	40.0	41.0	38.0	41.0
22	39.73991731804622	41.0	40.0	41.0	38.0	41.0
23	39.6679360809467	41.0	40.0	41.0	37.0	41.0
24	39.63551500913186	41.0	40.0	41.0	37.0	41.0
25	39.63744042501773	41.0	40.0	41.0	37.0	41.0
26	39.538790593194484	41.0	40.0	41.0	37.0	41.0
27	39.487713588444336	41.0	40.0	41.0	36.0	41.0
28	39.40498904573061	41.0	39.0	41.0	36.0	41.0
29	39.34825563659558	41.0	39.0	41.0	36.0	41.0
30	39.275802949927304	41.0	39.0	41.0	35.0	41.0
31	39.23056062881072	41.0	39.0	41.0	35.0	41.0
32	39.1521950137275	41.0	39.0	41.0	35.0	41.0
33	39.05608665163839	41.0	39.0	41.0	35.0	41.0
34	38.992308240859224	41.0	39.0	41.0	35.0	41.0
35	38.8821316651678	41.0	39.0	41.0	35.0	41.0
36	38.81255521704508	40.0	38.0	41.0	35.0	41.0
37	38.73864460229861	40.0	38.0	41.0	35.0	41.0
38	38.65112434779508	40.0	38.0	41.0	35.0	41.0
39	38.56521256829085	40.0	38.0	41.0	35.0	41.0
40	38.4492032898464	40.0	38.0	41.0	35.0	41.0
41	38.35609794265747	40.0	37.0	41.0	35.0	41.0
42	38.20937412098426	40.0	37.0	41.0	34.0	41.0
43	37.39265014084061	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	1.0
13	1.0
14	0.0
15	0.0
16	6.0
17	11.0
18	13.0
19	24.0
20	67.0
21	122.0
22	247.0
23	381.0
24	643.0
25	987.0
26	1471.0
27	2101.0
28	2970.0
29	4133.0
30	5421.0
31	7021.0
32	9009.0
33	11934.0
34	18223.0
35	27683.0
36	45142.0
37	86541.0
38	192782.0
39	592713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.86283590781774	17.776421975096373	11.8676534092935	26.49308870779239
2	20.531529675571385	19.54346646171156	31.96487502624667	27.96012883647039
3	20.89571456303756	21.016350187985562	28.149005796056464	29.93892945292041
4	15.146902100921903	15.150368641868683	32.26220519545348	37.44052406175593
5	17.212465285068518	33.85542741459434	31.386655996323487	17.54545130401366
6	35.94703917785534	32.64699124054625	15.34271214240154	16.06325743919687
7	31.05406615348655	28.83369715505937	19.84129185105363	20.270944840400453
8	29.173220079789868	30.026484372833412	19.20503302127862	21.595262526098104
9	27.462729732620744	13.793762603352441	19.20770721000899	39.535800454017824
10	19.661427897929187	23.988859527837313	29.679929322182296	26.6697832520512
11	38.746815734530315	20.231723405688296	19.543862637819764	21.477598221961628
12	23.881792934595286	24.24885009884594	25.989746962319693	25.879610004239083
13	31.458561959962445	17.40966194292687	23.60090407387892	27.530872023231765
14	24.374536969173537	19.761561409277654	22.39672679299402	33.46717482855479
15	27.64071184923122	25.626453471096973	20.19349241124665	26.53934226842516
16	27.562962287996264	23.843264808072483	22.28371755812894	26.310055345802315
17	26.21735013648267	24.47140202762932	22.900464714574923	26.410783121313088
18	26.60253235768364	22.27708160831653	24.5347902049419	26.585595829057933
19	26.471893286003496	23.672512905436722	23.758978341052163	26.09661546750762
20	26.747037593150907	22.443475573762047	23.68637906922385	27.123107763863192
21	28.573805628077793	23.17154821661325	23.128563108873156	25.126083046435806
22	28.12711706607821	22.598677564150815	22.927602777986873	26.3466025917841
23	26.381664177360122	22.498147876694148	23.877335953377994	27.242851992567736
24	26.147821229492934	23.71480470498746	23.457785454790365	26.679588610729244
25	26.399393058202232	22.60095557677299	23.511467317451952	27.48818404757283
26	26.48239195287089	23.95766065931628	23.675682314302353	25.884265073510477
27	27.295345326904712	22.43485874340862	23.348341804899114	26.92145412478755
28	25.7389674858268	23.132524869955194	24.60481433206689	26.52369331215112
29	25.32139786778019	23.360920396334578	24.816174285793522	26.501507450091715
30	25.082998894668656	23.960334848046656	24.80597275100728	26.150693506277413
31	26.445250442726802	23.57386505449402	23.27673297334131	26.704151529437866
32	25.636357873802062	22.888381343274713	23.64309682940261	27.832163953520617
33	24.95533114380004	22.470217461065793	24.798742537032563	27.775708858101606
34	26.43752500861683	22.43723580005784	24.49863913506832	26.626600056257004
35	26.412863045881156	22.38028548450357	25.214133186484055	25.992718283131218
36	24.638192169183046	23.367160170038787	24.958104376557465	27.036543284220706
37	25.54989243818662	22.03987116352961	25.670528063134622	26.739708335149142
38	24.53706821756407	21.565252185901677	26.69355381854342	27.204125777990836
39	24.937998439066135	21.303280734352033	26.512798469175518	27.245922357406315
40	24.312832540320823	22.016397729118548	27.25424205567859	26.41652767488204
41	23.28455745147833	22.17853280140088	28.181987457064416	26.354922290056376
42	24.2939151311541	22.610265715315773	27.668741308886624	25.427077844643502
43	22.843118222912448	21.514739732105713	27.961911628957303	27.680230416024532
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	115.0
1	112.5
2	110.0
3	267.0
4	424.0
5	424.0
6	531.5
7	639.0
8	634.5
9	630.0
10	916.5
11	1203.0
12	1203.0
13	1936.5
14	2670.0
15	3730.0
16	4790.0
17	4495.5
18	4201.0
19	4201.0
20	4806.5
21	5412.0
22	4679.5
23	3947.0
24	4634.5
25	5322.0
26	5322.0
27	6192.0
28	7062.0
29	8606.5
30	10151.0
31	12352.0
32	14553.0
33	14553.0
34	18074.0
35	21595.0
36	26581.0
37	31567.0
38	36544.5
39	41522.0
40	41522.0
41	45036.0
42	48550.0
43	51283.5
44	54017.0
45	58338.5
46	62660.0
47	62660.0
48	67208.5
49	71757.0
50	72916.5
51	74076.0
52	75897.5
53	77719.0
54	77719.0
55	76820.5
56	75922.0
57	74137.0
58	72352.0
59	71467.0
60	70582.0
61	70582.0
62	65860.5
63	61139.0
64	56289.0
65	51439.0
66	46237.5
67	41036.0
68	41036.0
69	35779.0
70	30522.0
71	26824.5
72	23127.0
73	17892.5
74	12658.0
75	12658.0
76	10188.0
77	7718.0
78	6625.0
79	5532.0
80	4983.0
81	4434.0
82	4434.0
83	3640.0
84	2846.0
85	2689.0
86	2532.0
87	2319.0
88	2106.0
89	2106.0
90	1493.0
91	880.0
92	489.0
93	98.0
94	63.0
95	28.0
96	28.0
97	18.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1009652.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.00736579296671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.78419883978852	48.83784902215738
2	6.193254539839963	6.813492358438572
3	1.7681708233151028	2.9178725778763486
4	0.8268220380904594	1.8192520917971262
5	0.48020199589799906	1.3207323421436965
6	0.3214421315421572	1.0609010946606217
7	0.23218093499942932	0.8940163135166644
8	0.1641392718427336	0.7223095173795558
9	0.12331473502147844	0.6104896863290304
>10	0.8489979431570563	9.257481479088266
>50	0.12052282922069277	4.660052906959521
>100	0.12105788726962062	14.148203209723517
>500	0.013711474495750848	5.2461804131736525
>1k	0.001984555519121833	1.691166986756185
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2179	0.21581693494392126	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2122	0.2101714254020197	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1686	0.16698822960782528	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1659	0.16431404087745083	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1649	0.1633236006069418	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1475	0.1460899399000844	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1321	0.13083715973424506	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1279	0.12667731059810705	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1279	0.12667731059810705	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1249	0.12370598978657993	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1143	0.11320732291918403	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.904402705090467E-5
2	0.0	0.0	0.0	0.0	9.904402705090467E-5
3	0.0	0.0	0.0	0.0	9.904402705090467E-5
4	0.0	0.0	0.0	0.0	9.904402705090467E-5
5	0.0	9.904402705090467E-5	0.0	0.0	9.904402705090467E-5
6	0.0	9.904402705090467E-5	0.0	0.0	9.904402705090467E-5
7	0.0	9.904402705090467E-5	0.0	9.904402705090467E-5	9.904402705090467E-5
8	0.0	9.904402705090467E-5	0.0	9.904402705090467E-5	1.9808805410180934E-4
9	0.0	1.9808805410180934E-4	0.0	2.97132081152714E-4	1.9808805410180934E-4
10	0.0	1.9808805410180934E-4	0.0	2.97132081152714E-4	1.9808805410180934E-4
11	0.0	1.9808805410180934E-4	0.0	4.952201352545234E-4	1.9808805410180934E-4
12	0.0	1.9808805410180934E-4	0.0	6.933081893563327E-4	1.9808805410180934E-4
13	0.0	1.9808805410180934E-4	0.0	6.933081893563327E-4	2.97132081152714E-4
14	0.0	1.9808805410180934E-4	0.0	7.923522164072374E-4	2.97132081152714E-4
15	0.0	1.9808805410180934E-4	0.0	0.0010894842975599514	2.97132081152714E-4
16	0.0	1.9808805410180934E-4	0.0	0.002079924568068998	3.961761082036187E-4
17	0.0	1.9808805410180934E-4	0.0	0.0024761006762726166	3.961761082036187E-4
18	0.0	1.9808805410180934E-4	0.0	0.002674188730374426	3.961761082036187E-4
19	0.0	1.9808805410180934E-4	0.0	0.003268452892679854	3.961761082036187E-4
20	0.0	1.9808805410180934E-4	0.0	0.004060805109087092	3.961761082036187E-4
21	0.0	1.9808805410180934E-4	0.0	0.005546465514850662	3.961761082036187E-4
22	0.0	1.9808805410180934E-4	0.0	0.007626390082919659	3.961761082036187E-4
23	0.0	2.97132081152714E-4	0.0	0.0105977108944468	3.961761082036187E-4
24	0.0	2.97132081152714E-4	0.0	0.017530792788010126	3.961761082036187E-4
25	0.0	2.97132081152714E-4	0.0	0.021987774005300838	3.961761082036187E-4
26	0.0	2.97132081152714E-4	0.0	0.03476445349486754	3.961761082036187E-4
27	0.0	2.97132081152714E-4	0.0	0.08844631615645787	3.961761082036187E-4
28	0.0	4.952201352545234E-4	0.0	0.2651408604152718	3.961761082036187E-4
29	0.0	4.952201352545234E-4	0.0	0.47253905305986615	3.961761082036187E-4
30	0.0	4.952201352545234E-4	0.0	0.7591724673451843	3.961761082036187E-4
31	0.0	4.952201352545234E-4	0.0	1.341353258350402	3.961761082036187E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1290	0.0	20.794575	1
CTCTATA	45	0.0038254906	20.555555	2
CTAACAC	55	5.1424996E-4	20.181818	3
CGAGCTA	80	1.6163536E-5	18.5	22
ACACTTA	85	2.7225838E-5	17.411764	6
CACTTAC	85	2.7225838E-5	17.411764	7
AAGACGG	430	0.0	16.77907	5
ATACTGA	100	5.8763653E-6	16.650002	6
CAAGACG	455	0.0	16.263735	4
TCGTTTA	285	0.0	16.22807	30
ATATAGC	70	0.0025922004	15.857143	6
CGAAACC	105	9.340767E-6	15.857142	12
GTATCAA	1680	0.0	15.857142	2
AGACGGA	445	0.0	15.797752	6
GACGGAC	450	0.0	15.622222	7
ATCTTAC	85	5.3633476E-4	15.235294	1
GTTCAAA	500	0.0	15.170001	1
AGACCGT	110	1.4514417E-5	15.136364	6
ACGGACC	470	0.0	14.957446	8
GTACTAT	100	1.09315435E-4	14.8	1
>>END_MODULE