FastQCFastQC Report
Fri 10 Feb 2017
ERR1631604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487560
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT375391.5090691279808326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT322301.2956471401694833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT290531.1679316277798324No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134780.5418160767981476No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA44020.17696055572528904No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA38440.15452893598546366No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37720.15163453343838942No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36810.1479763302191706No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35720.1435945263631832No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA32300.12984611426458054No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28700.11537410152920935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA902.1542655E-618.52
AGTCGGT27300.018.3644711
GCAGTCG27800.018.2338129
CAGTCGG28300.018.04240210
GCGCTAT752.068486E-417.2666661
GGCAGTC29700.017.191928
GGGCAGT30000.016.9583347
TCGGTGA29500.016.93220313
GTCGGTG29650.016.72175412
TAATACG1005.8833284E-616.654
CGGTGAT30900.016.2249214
CGCCTTA20900.016.19856525
CGAACTA1151.2435412E-616.08695629
GGTATCA178500.015.8053221
CGCTTAT8850.015.67796726
GTGATTC31600.015.57278616
AAGACGG5350.015.5607475
GCCTTAT21350.015.51053826
CCGCTTA9100.015.45054925
TCGCCTT22200.015.33333324
Produced by FastQC (version 0.11.5)