FastQCFastQC Report
Fri 10 Feb 2017
ERR1631603.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631603.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278950
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11700.4194300053773078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10220.36637390213299875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8200.2939594909481986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.1656210790464241No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4100.1469797454740993No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3070.11005556551353288No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3060.10969707832944972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAC250.00548929829.627
GACTGAC405.9242593E-527.7500027
TCCATTA603.71328E-521.5833348
GGTATCA4800.021.5833341
GTCCTAT806.921309E-720.81251
TTCAGGG450.003818657520.5555552
ATTGCCG450.003818657520.55555524
ATACACA1457.2759576E-1219.1379337
TAGAGTG609.212932E-418.55
GCGGTAT701.2150021E-418.524
TTAGAGT701.2150021E-418.54
TGCGGTA701.2150021E-418.523
TAACGAA701.2150021E-418.513
AACGAAC701.2150021E-418.514
ATAACGA701.2150021E-418.512
CGGTCCA801.6095295E-518.510
CCATTAT701.2150021E-418.59
CGAACGA752.0602037E-417.26666616
TCCGATA752.0602037E-417.2666668
AGCTGCG752.0602037E-417.26666620
Produced by FastQC (version 0.11.5)