##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631603.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278950
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.025151460835275	34.0	33.0	34.0	31.0	34.0
2	33.16804803728267	34.0	33.0	34.0	31.0	34.0
3	33.24856425882775	34.0	34.0	34.0	31.0	34.0
4	36.47210611220649	37.0	37.0	37.0	35.0	37.0
5	36.464599390571784	37.0	37.0	37.0	35.0	37.0
6	36.52606201828284	37.0	37.0	37.0	35.0	37.0
7	36.533776662484314	37.0	37.0	37.0	35.0	37.0
8	36.53705682021868	37.0	37.0	37.0	35.0	37.0
9	38.37926510127263	39.0	39.0	39.0	37.0	39.0
10	38.33802115074386	39.0	39.0	39.0	37.0	39.0
11	38.38961821114895	39.0	39.0	39.0	37.0	39.0
12	38.35604230148772	39.0	39.0	39.0	37.0	39.0
13	38.385599569815376	39.0	39.0	39.0	37.0	39.0
14	39.92386449184442	41.0	40.0	41.0	38.0	41.0
15	39.91119913963076	41.0	40.0	41.0	38.0	41.0
16	39.872263846567485	41.0	40.0	41.0	38.0	41.0
17	39.87135328911992	41.0	40.0	41.0	38.0	41.0
18	39.8371464420147	41.0	40.0	41.0	38.0	41.0
19	39.85585230328016	41.0	40.0	41.0	38.0	41.0
20	39.83677003047141	41.0	40.0	41.0	38.0	41.0
21	39.802423373364405	41.0	40.0	41.0	38.0	41.0
22	39.73540060942821	41.0	40.0	41.0	38.0	41.0
23	39.66991575551174	41.0	40.0	41.0	37.0	41.0
24	39.642520164904106	41.0	40.0	41.0	37.0	41.0
25	39.62872557806058	41.0	40.0	41.0	37.0	41.0
26	39.52186054848539	41.0	40.0	41.0	37.0	41.0
27	39.45897114178168	41.0	39.0	41.0	36.0	41.0
28	39.389664814482884	41.0	39.0	41.0	36.0	41.0
29	39.3267861623947	41.0	39.0	41.0	36.0	41.0
30	39.24555655135329	41.0	39.0	41.0	35.0	41.0
31	39.18276393618928	41.0	39.0	41.0	35.0	41.0
32	39.08808030112923	41.0	39.0	41.0	35.0	41.0
33	38.99243592041584	41.0	39.0	41.0	35.0	41.0
34	38.92494712314035	41.0	39.0	41.0	35.0	41.0
35	38.81278723785625	40.0	39.0	41.0	35.0	41.0
36	38.73963434307223	40.0	38.0	41.0	35.0	41.0
37	38.6698333034594	40.0	38.0	41.0	35.0	41.0
38	38.57644022226206	40.0	38.0	41.0	35.0	41.0
39	38.46224412977236	40.0	38.0	41.0	35.0	41.0
40	38.346875784190715	40.0	38.0	41.0	35.0	41.0
41	38.24922387524646	40.0	38.0	41.0	35.0	41.0
42	38.1003513174404	40.0	37.0	41.0	34.0	41.0
43	37.30150564617315	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	4.0
17	1.0
18	7.0
19	14.0
20	23.0
21	35.0
22	65.0
23	134.0
24	185.0
25	297.0
26	404.0
27	644.0
28	886.0
29	1277.0
30	1667.0
31	2071.0
32	2604.0
33	3451.0
34	5152.0
35	7653.0
36	12208.0
37	24486.0
38	53786.0
39	161892.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10880086036924	18.291450080659615	12.795124574296468	25.804624484674672
2	19.68883312421581	20.030112923462987	33.36440222262054	26.916651729700664
3	20.649220290374622	21.53611758379638	28.884029395949096	28.930632729879907
4	15.142140168488977	15.91647248610862	32.8689729342176	36.0724144111848
5	16.252733464778636	34.70442731672343	32.140168488976514	16.902670729521418
6	34.52052339128876	33.262591862340926	16.01039612833841	16.206488618031905
7	29.9211328195017	29.632909123498834	20.367449363685246	20.078508693314216
8	28.465674852124035	30.937085499193408	19.762681484136944	20.834558164545616
9	27.100555655135327	14.234450618390394	19.891736870406884	38.7732568560674
10	19.290195375515324	25.23176196450977	30.615880982254883	24.86216167772002
11	36.461373005915036	21.639003405628248	20.040509051801397	21.859114536655312
12	22.86323713927227	25.359383402043377	27.230328015773438	24.547051442910917
13	30.867180498297188	18.593654776841728	24.694748162753182	25.844416562107902
14	23.771643663739024	20.642050546692957	23.91754794766087	31.66875784190715
15	26.825954472127624	26.32443090159527	21.435741172253092	25.413873454024017
16	25.94801935830794	25.207026348808032	23.522136583617137	25.322817709266893
17	24.569636135508155	25.750851407062196	24.341996773615342	25.337515683814303
18	24.851048575013444	23.530740276035132	26.18031905359383	25.43789209535759
19	24.894067037103422	24.800501882057716	25.906076357770207	24.39935472306865
20	25.565155045707115	23.52034414769672	25.723247893887795	25.19125291270837
21	27.096253808926328	23.97813228177093	24.789747266535223	24.13586664276752
22	26.573221007348984	23.623946943896755	24.651012726295036	25.151819322459225
23	25.22244129772361	23.647248610862164	25.20164904104678	25.928661050367452
24	24.737408137659077	24.56354185337874	25.39917547947661	25.29987452948557
25	25.063272987990683	23.684531278006812	25.30596881161499	25.94622692238752
26	25.17440401505646	24.75569098404732	25.198781143574116	24.8711238573221
27	25.678436995877398	23.691701021688473	25.02527334647786	25.604588635956265
28	24.472127621437533	24.264205054669297	26.019716795124577	25.243950528768593
29	23.940849614626277	24.465316364939955	26.358487184083167	25.2353468363506
30	23.80319053593834	25.144291091593473	26.365298440580748	24.687219931887437
31	25.089801039612837	24.81376590786879	24.91091593475533	25.18551711776304
32	24.098942462806956	24.148413694210433	25.715361175837963	26.037282667144652
33	23.87309553683456	23.726115791360456	26.43807133894963	25.96271733285535
34	25.033160064527692	23.423552607994264	26.097867001254706	25.44542032622334
35	24.635239290195376	23.383043556192867	26.902312242337334	25.079404911274423
36	23.328553504212223	23.970962538089264	26.731313855529663	25.96917010216885
37	24.08926330883671	22.480372826671445	27.406345223158272	26.024018641333573
38	23.060763577702097	22.108980103961283	28.621258290016133	26.208998028320487
39	23.38949632550636	22.1405269761606	28.78938877935114	25.680587918981896
40	22.578239827926154	22.40724144111848	29.133536476070983	25.880982254884387
41	21.709266893708552	22.694031188385015	30.08603692417996	25.510664993726472
42	22.284997311346117	22.900161319232836	29.584154866463525	25.230686502957518
43	20.98512278186055	22.46531636493995	29.763398458505108	26.78616239469439
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	69.0
2	65.0
3	162.0
4	259.0
5	259.0
6	295.5
7	332.0
8	320.0
9	308.0
10	471.5
11	635.0
12	635.0
13	1084.5
14	1534.0
15	2083.0
16	2632.0
17	2396.5
18	2161.0
19	2161.0
20	2516.0
21	2871.0
22	2513.5
23	2156.0
24	2481.0
25	2806.0
26	2806.0
27	3027.0
28	3248.0
29	3960.0
30	4672.0
31	5312.0
32	5952.0
33	5952.0
34	6902.5
35	7853.0
36	9066.0
37	10279.0
38	11299.0
39	12319.0
40	12319.0
41	13178.0
42	14037.0
43	14609.0
44	15181.0
45	16224.0
46	17267.0
47	17267.0
48	18315.0
49	19363.0
50	19621.0
51	19879.0
52	20503.0
53	21127.0
54	21127.0
55	20302.0
56	19477.0
57	18822.5
58	18168.0
59	17333.5
60	16499.0
61	16499.0
62	15531.5
63	14564.0
64	13521.5
65	12479.0
66	11052.0
67	9625.0
68	9625.0
69	8342.5
70	7060.0
71	6158.5
72	5257.0
73	4129.0
74	3001.0
75	3001.0
76	2475.0
77	1949.0
78	1640.0
79	1331.0
80	1136.0
81	941.0
82	941.0
83	772.0
84	603.0
85	531.5
86	460.0
87	399.5
88	339.0
89	339.0
90	248.0
91	157.0
92	91.5
93	26.0
94	14.5
95	3.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	278950.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.22185109266307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.07860191026141	62.13550817249792
2	4.619584109272253	6.303131584256083
3	1.3698096641008939	2.8035285278874595
4	0.6787109921398101	1.8521168096286296
5	0.4297114057365151	1.4657853767487727
6	0.30320936249167996	1.241130238668521
7	0.19924490423998542	0.9514999331622538
8	0.16456159056438804	0.8981357061644374
9	0.1256744448749931	0.7716368937973386
>10	0.8912529380203893	11.798038695486422
>50	0.09695666719533332	4.383586142640661
>100	0.0411011958762826	4.313126663884096
>500	5.269384086702898E-4	0.2947794519407366
>1k	0.0010538768173405796	0.7879958032367008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1170	0.4194300053773078	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1022	0.36637390213299875	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	820	0.2939594909481986	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	462	0.1656210790464241	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	410	0.1469797454740993	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	307	0.11005556551353288	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	306	0.10969707832944972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.58487184083169E-4	0.0
11	0.0	0.0	0.0	3.58487184083169E-4	0.0
12	0.0	0.0	0.0	0.0010754615522495072	0.0
13	0.0	0.0	0.0	0.0010754615522495072	0.0
14	0.0	3.58487184083169E-4	0.0	0.001433948736332676	0.0
15	0.0	3.58487184083169E-4	0.0	0.001792435920415845	0.0
16	0.0	3.58487184083169E-4	0.0	0.003226384656748521	0.0
17	0.0	3.58487184083169E-4	0.0	0.004301846208998029	0.0
18	0.0	3.58487184083169E-4	0.0	0.004301846208998029	0.0
19	0.0	3.58487184083169E-4	0.0	0.004301846208998029	0.0
20	0.0	3.58487184083169E-4	0.0	0.005735794945330704	0.0
21	0.0	3.58487184083169E-4	0.0	0.00716974368166338	0.0
22	0.0	3.58487184083169E-4	0.0	0.012188564258827746	0.0
23	0.0	3.58487184083169E-4	0.0	0.016131923283742605	0.0
24	0.0	3.58487184083169E-4	0.0	0.025452590069905003	0.0
25	0.0	3.58487184083169E-4	0.0	0.03154687219931888	0.0
26	0.0	3.58487184083169E-4	0.0	0.04481089801039613	0.0
27	0.0	3.58487184083169E-4	0.0	0.1075461552249507	0.0
28	0.0	3.58487184083169E-4	0.0	0.2961104140526976	0.0
29	0.0	3.58487184083169E-4	0.0	0.5384477504929199	0.0
30	0.0	3.58487184083169E-4	0.0	0.8833124215809285	0.0
31	0.0	3.58487184083169E-4	0.0	1.6336260978670012	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGAAC	25	0.005489298	29.6	27
GACTGAC	40	5.9242593E-5	27.750002	7
TCCATTA	60	3.71328E-5	21.583334	8
GGTATCA	480	0.0	21.583334	1
GTCCTAT	80	6.921309E-7	20.8125	1
TTCAGGG	45	0.0038186575	20.555555	2
ATTGCCG	45	0.0038186575	20.555555	24
ATACACA	145	7.2759576E-12	19.13793	37
TAGAGTG	60	9.212932E-4	18.5	5
GCGGTAT	70	1.2150021E-4	18.5	24
TTAGAGT	70	1.2150021E-4	18.5	4
TGCGGTA	70	1.2150021E-4	18.5	23
TAACGAA	70	1.2150021E-4	18.5	13
AACGAAC	70	1.2150021E-4	18.5	14
ATAACGA	70	1.2150021E-4	18.5	12
CGGTCCA	80	1.6095295E-5	18.5	10
CCATTAT	70	1.2150021E-4	18.5	9
CGAACGA	75	2.0602037E-4	17.266666	16
TCCGATA	75	2.0602037E-4	17.266666	8
AGCTGCG	75	2.0602037E-4	17.266666	20
>>END_MODULE