FastQCFastQC Report
Fri 10 Feb 2017
ERR1631602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658666
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT276594.199245140936378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT215483.2714608010736854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT196512.983454436694774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94001.4271269505333508No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA27160.41234859549452985No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26340.39989919018136655No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26270.39883643606926733No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.38896800502834517No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.31761165750167764No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA20580.3124497089571953No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA18420.2796561534981311No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15750.23911967522234331No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.21619455080420122No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.20025323912271167No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA12910.19600222267431444No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12530.19023298606577538No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.17596171656044185No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT11230.17049612398393116No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT11210.1701924799519028No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9650.14650824545368973No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA9250.1404353648131223No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT9150.13891714465298044No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8990.13648799239675344No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA8770.13314790804444135No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT8450.12828960353198737No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA8360.1269232053878597No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA8290.1258604512757604No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA8070.1225203669234483No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7680.11659930829889505No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA7580.11508108813875317No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA7550.11462562209071062No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAA7380.11204464781846946No Hit
GTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7240.10991913959427085No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT7010.10642723322594456No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT6940.10536447911384525No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT6910.1049090130658027No Hit
CAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA6850.10399808096971759No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT6690.1015689287134906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTGG200.001841365237.023
TTTAGAG303.597824E-430.8333342
TTTGAGT303.597824E-430.8333344
TTAAGCT358.865358E-426.428574
ATATGCT659.379073E-825.61538535
TATGCTG601.3359586E-624.66666836
TTTGAGC451.3223746E-424.6666663
TATTGAC1250.023.6830
TACCTTA400.001930316123.12500231
TAAGCTG400.001930316123.1250025
ATTGACT1350.021.92592631
TTTGGAG1301.8189894E-1221.3461532
TTTACCA450.003824100620.5555552
TTTGTAG450.003824100620.5555552
TTTAAGC450.003824100620.5555553
CCAGAAC555.1398895E-420.1818183
GAACCAT555.1398895E-420.1818186
GTAGTAT555.1398895E-420.1818183
GGCAGTC13600.019.9963258
AGGGCAA2500.019.9810
Produced by FastQC (version 0.11.5)