##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631602.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658666
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94352069182256	34.0	33.0	34.0	31.0	34.0
2	32.996717608013775	34.0	33.0	34.0	31.0	34.0
3	32.99965232758333	34.0	33.0	34.0	31.0	34.0
4	36.320298907185126	37.0	37.0	37.0	35.0	37.0
5	36.36386575290056	37.0	37.0	37.0	35.0	37.0
6	36.50645091746044	37.0	37.0	37.0	35.0	37.0
7	36.48680514858821	37.0	37.0	37.0	35.0	37.0
8	36.540269271527606	37.0	37.0	37.0	35.0	37.0
9	38.33102513261653	39.0	39.0	39.0	37.0	39.0
10	38.3313272584284	39.0	39.0	39.0	37.0	39.0
11	38.38399279756356	39.0	39.0	39.0	37.0	39.0
12	38.38092447461992	39.0	39.0	39.0	37.0	39.0
13	38.40417449815233	39.0	39.0	39.0	37.0	39.0
14	39.748324644053284	41.0	40.0	41.0	37.0	41.0
15	39.676811919850124	41.0	39.0	41.0	37.0	41.0
16	39.57518833521087	41.0	39.0	41.0	37.0	41.0
17	39.72307360634981	41.0	39.0	41.0	38.0	41.0
18	39.8487002517209	41.0	40.0	41.0	38.0	41.0
19	39.90786680958179	41.0	40.0	41.0	38.0	41.0
20	39.897524693850904	41.0	40.0	41.0	38.0	41.0
21	39.85665724358021	41.0	40.0	41.0	37.0	41.0
22	39.72644405510532	41.0	40.0	41.0	37.0	41.0
23	39.6023189294726	41.0	40.0	41.0	35.0	41.0
24	39.51851469485293	41.0	39.0	41.0	35.0	41.0
25	39.477039956518176	41.0	39.0	41.0	35.0	41.0
26	39.21255537708034	41.0	39.0	41.0	35.0	41.0
27	39.00963007047578	41.0	38.0	41.0	35.0	41.0
28	38.82341884961422	41.0	38.0	41.0	35.0	41.0
29	38.56367567173651	40.0	37.0	41.0	35.0	41.0
30	38.216656089732886	40.0	36.0	41.0	35.0	41.0
31	37.81152511287967	39.0	35.0	41.0	35.0	41.0
32	37.46504449903289	39.0	35.0	41.0	35.0	41.0
33	37.139160059878606	39.0	35.0	41.0	35.0	41.0
34	36.879003926117335	39.0	35.0	41.0	34.0	41.0
35	36.57073084082069	39.0	35.0	41.0	33.0	41.0
36	36.24067129622601	38.0	35.0	41.0	33.0	41.0
37	35.87018762164739	38.0	35.0	41.0	32.0	41.0
38	35.498323884943204	37.0	35.0	41.0	30.0	41.0
39	35.05206128751143	37.0	35.0	41.0	25.0	41.0
40	34.5667956141656	37.0	35.0	41.0	21.0	41.0
41	34.01386590472258	36.0	35.0	41.0	15.0	41.0
42	33.41340072206551	35.0	35.0	41.0	10.0	41.0
43	32.427842032228774	35.0	33.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	14.0
11	10.0
12	12.0
13	12.0
14	6.0
15	13.0
16	13.0
17	21.0
18	24.0
19	33.0
20	75.0
21	154.0
22	276.0
23	454.0
24	833.0
25	1328.0
26	2211.0
27	3709.0
28	5595.0
29	8202.0
30	10899.0
31	12186.0
32	13877.0
33	16582.0
34	22227.0
35	28858.0
36	44133.0
37	135630.0
38	122132.0
39	229144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.08309826224521	15.091412035842142	16.35092748069583	17.474562221216825
2	18.734533132118557	25.243294780662733	33.3272098453541	22.694962241864616
3	21.75897951313716	28.39542347714927	31.136418154269386	18.709178855444186
4	13.443839518056192	18.576030947399744	29.43373424467029	38.54639528987377
5	12.039334047908955	41.612440903280266	28.352609668633267	17.99561538017751
6	26.609085636726356	32.437532831510964	19.355940643664617	21.597440888098067
7	24.56829409746366	35.9171416165401	19.360950770193085	20.153613515803155
8	34.60752490640173	28.171789647560374	18.150625658527993	19.07005978750991
9	30.573765762920814	11.436600644332636	19.39617347790838	38.59346011483817
10	21.61535588598774	30.621286053933254	22.030133633738497	25.733224426340513
11	29.824979579938848	27.439400242307936	16.30340718968339	26.432212988069825
12	22.01328138996092	35.98652427785858	24.469154321006396	17.531040011174102
13	33.838242751257845	20.645820491721146	26.481858787306468	19.034077969714545
14	23.3497705969338	24.531401347572217	29.586922658828602	22.53190539666538
15	28.691476408376932	26.25822495771757	25.971736813498797	19.0785618204067
16	17.99242711784121	27.464602696966296	30.832166834176956	23.71080335101554
17	16.434885055551675	33.00959818785242	28.5680451093574	21.98747164723851
18	17.195513355782747	24.419812165801787	35.063750064524356	23.320924413891106
19	18.544451968068792	28.62710387358692	37.76390461933666	15.06453953900763
20	20.994403840489717	24.7677882265063	38.42508949907844	15.812718433925541
21	24.452302077228826	23.867331849526163	35.67802194131776	16.00234413192726
22	22.594759711295254	25.793801410730172	33.21637977366374	18.395059104310835
23	19.115758214330175	28.090261224960756	34.130044666037115	18.66393589467196
24	17.011960538421597	29.451801064575978	36.34649427782822	17.18974411917421
25	16.98053338110666	28.2180953624447	34.68996426109743	20.11140699535121
26	19.098450504504562	26.98939978684189	35.166685391381975	18.745464317271576
27	18.197386839460364	28.04000813766006	33.91855659772935	19.84404842515023
28	16.680684899478642	28.418196779551398	37.536323417331396	17.36479490363857
29	18.009886649682844	28.43611177744107	37.401505467110795	16.15249610576529
30	16.94925804580774	29.204179356456837	37.97478539958036	15.87177719815506
31	17.06600917612265	30.420880992794526	35.81435811169849	16.69875171938433
32	15.49753592868009	30.485557171616573	38.297862649658555	15.719044250044787
33	15.221068037518256	30.33722706197071	38.45074741978484	15.990957480726195
34	15.766716363073241	30.411468027801646	35.19219149007236	18.62962411905275
35	14.508719138379695	31.517643236481007	35.24244457737306	18.731193047766244
36	13.636198012346165	30.757470402297976	35.23606805270046	20.370263532655397
37	14.020915000926115	30.999626517840607	35.70216164186401	19.27729683936927
38	14.542879091982886	28.726091828028167	36.56754713314487	20.163481946844076
39	14.571269808977538	28.653824548405417	37.84771037217649	18.92719527044056
40	14.546219176335198	28.725484539964107	36.34057321920366	20.387723064497028
41	12.663018889695232	29.716123194456674	37.98632387279744	19.63453404305065
42	12.60881842997817	29.244867656748642	36.61749657641354	21.528817336859653
43	11.823291319120768	30.677612021874516	37.077517284936526	20.42157937406819
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83.0
1	220.0
2	357.0
3	1651.0
4	2945.0
5	2945.0
6	4434.0
7	5923.0
8	6145.0
9	6367.0
10	10138.5
11	13910.0
12	13910.0
13	22082.5
14	30255.0
15	43515.0
16	56775.0
17	48579.5
18	40384.0
19	40384.0
20	41002.0
21	41620.0
22	28003.0
23	14386.0
24	13277.5
25	12169.0
26	12169.0
27	11765.5
28	11362.0
29	11631.0
30	11900.0
31	11615.0
32	11330.0
33	11330.0
34	10205.0
35	9080.0
36	8798.0
37	8516.0
38	10364.5
39	12213.0
40	12213.0
41	14729.0
42	17245.0
43	21995.5
44	26746.0
45	35667.5
46	44589.0
47	44589.0
48	50631.5
49	56674.0
50	48278.5
51	39883.0
52	32067.0
53	24251.0
54	24251.0
55	21617.0
56	18983.0
57	19324.0
58	19665.0
59	19786.0
60	19907.0
61	19907.0
62	19872.5
63	19838.0
64	19604.0
65	19370.0
66	18371.0
67	17372.0
68	17372.0
69	15967.5
70	14563.0
71	12967.0
72	11371.0
73	9792.0
74	8213.0
75	8213.0
76	6689.0
77	5165.0
78	3977.0
79	2789.0
80	2120.0
81	1451.0
82	1451.0
83	1048.0
84	645.0
85	441.5
86	238.0
87	169.0
88	100.0
89	100.0
90	61.0
91	22.0
92	15.5
93	9.0
94	5.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	658666.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.793600766590984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.5767253193358	40.10453595719063
2	6.778485886605908	6.208225529864048
3	1.8007532744855697	2.4738892959277083
4	0.866088960273651	1.5864532830049378
5	0.5248353048655553	1.2017049209612651
6	0.38712390366439603	1.0636678494966947
7	0.26300047931940906	0.8430617265962561
8	0.22212817789869937	0.8137639278162668
9	0.15544907153258156	0.6406715449170254
>10	1.148667103716216	10.473466439447591
>50	0.15411409729346534	4.896800559257634
>100	0.10307415928041087	9.655639530720864
>500	0.013257126595551236	4.0937701796968495
>1k	0.004971422473331714	4.066906378050524
>5k	3.3142816488878095E-4	1.4266651721777477
>10k+	9.942844946663426E-4	10.450777704873973
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27659	4.199245140936378	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21548	3.2714608010736854	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19651	2.983454436694774	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9400	1.4271269505333508	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	2716	0.41234859549452985	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2634	0.39989919018136655	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2627	0.39883643606926733	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2562	0.38896800502834517	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2092	0.31761165750167764	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	2058	0.3124497089571953	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1842	0.2796561534981311	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1575	0.23911967522234331	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1424	0.21619455080420122	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1319	0.20025323912271167	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	1291	0.19600222267431444	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1253	0.19023298606577538	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1159	0.17596171656044185	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	1123	0.17049612398393116	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	1121	0.1701924799519028	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	965	0.14650824545368973	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	925	0.1404353648131223	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	915	0.13891714465298044	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	899	0.13648799239675344	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	877	0.13314790804444135	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	845	0.12828960353198737	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	836	0.1269232053878597	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	829	0.1258604512757604	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	807	0.1225203669234483	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	768	0.11659930829889505	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	758	0.11508108813875317	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	755	0.11462562209071062	No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAA	738	0.11204464781846946	No Hit
GTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	724	0.10991913959427085	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	701	0.10642723322594456	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	694	0.10536447911384525	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	691	0.1049090130658027	No Hit
CAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA	685	0.10399808096971759	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	669	0.1015689287134906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	1.5182201601418626E-4	0.0	0.0
7	0.0	0.0	1.5182201601418626E-4	0.0	0.0
8	0.0	0.0	1.5182201601418626E-4	0.0	0.0
9	0.0	0.0	1.5182201601418626E-4	1.5182201601418626E-4	0.0
10	0.0	0.0	1.5182201601418626E-4	0.0015182201601418625	0.0
11	0.0	0.0	1.5182201601418626E-4	0.010779363137007223	1.5182201601418626E-4
12	0.0	0.0	1.5182201601418626E-4	0.0242915225622698	1.5182201601418626E-4
13	0.0	0.0	1.5182201601418626E-4	0.028998005058709572	1.5182201601418626E-4
14	0.0	0.0	1.5182201601418626E-4	0.03932190214767424	1.5182201601418626E-4
15	0.0	0.0	1.5182201601418626E-4	0.055111391813149606	3.036440320283725E-4
16	0.0	0.0	1.5182201601418626E-4	0.09306689581669617	3.036440320283725E-4
17	0.0	0.0	1.5182201601418626E-4	0.12950417966010086	3.036440320283725E-4
18	0.0	0.0	1.5182201601418626E-4	0.14529366932557625	3.036440320283725E-4
19	0.0	0.0	1.5182201601418626E-4	0.16806697172770418	3.036440320283725E-4
20	0.0	0.0	1.5182201601418626E-4	0.19554675662627188	3.036440320283725E-4
21	0.0	1.5182201601418626E-4	1.5182201601418626E-4	0.24018242933444264	3.036440320283725E-4
22	0.0	1.5182201601418626E-4	1.5182201601418626E-4	0.32292542806217417	3.036440320283725E-4
23	0.0	3.036440320283725E-4	1.5182201601418626E-4	0.43542554192868616	3.036440320283725E-4
24	0.0	3.036440320283725E-4	1.5182201601418626E-4	0.5880066680229433	3.036440320283725E-4
25	0.0	3.036440320283725E-4	1.5182201601418626E-4	0.6434217038681214	3.036440320283725E-4
26	0.0	3.036440320283725E-4	1.5182201601418626E-4	0.7121970771225477	3.036440320283725E-4
27	0.0	3.036440320283725E-4	1.5182201601418626E-4	0.8133105397879957	3.036440320283725E-4
28	0.0	4.554660480425587E-4	1.5182201601418626E-4	1.1072379627914604	3.036440320283725E-4
29	0.0	4.554660480425587E-4	1.5182201601418626E-4	1.5888173975884592	3.036440320283725E-4
30	0.0	4.554660480425587E-4	1.5182201601418626E-4	2.4130591225294764	3.036440320283725E-4
31	0.0	4.554660480425587E-4	1.5182201601418626E-4	3.7530402358706842	3.036440320283725E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCTGG	20	0.0018413652	37.0	23
TTTAGAG	30	3.597824E-4	30.833334	2
TTTGAGT	30	3.597824E-4	30.833334	4
TTAAGCT	35	8.865358E-4	26.42857	4
ATATGCT	65	9.379073E-8	25.615385	35
TATGCTG	60	1.3359586E-6	24.666668	36
TTTGAGC	45	1.3223746E-4	24.666666	3
TATTGAC	125	0.0	23.68	30
TACCTTA	40	0.0019303161	23.125002	31
TAAGCTG	40	0.0019303161	23.125002	5
ATTGACT	135	0.0	21.925926	31
TTTGGAG	130	1.8189894E-12	21.346153	2
TTTACCA	45	0.0038241006	20.555555	2
TTTGTAG	45	0.0038241006	20.555555	2
TTTAAGC	45	0.0038241006	20.555555	3
CCAGAAC	55	5.1398895E-4	20.181818	3
GAACCAT	55	5.1398895E-4	20.181818	6
GTAGTAT	55	5.1398895E-4	20.181818	3
GGCAGTC	1360	0.0	19.996325	8
AGGGCAA	250	0.0	19.98	10
>>END_MODULE