FastQCFastQC Report
Fri 10 Feb 2017
ERR1631601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509113
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8300.16302864000722825No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC6960.13670835354822997No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC5880.11549498834247014No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC5770.11333436781225385No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC5370.10547756588419467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACT1300.027.0384624
GTCTAGA1250.023.681
TTACTAT400.001929717823.12521
CTAGAAC1600.021.968753
TAGACTA603.7210397E-521.5833345
ACTGATC957.1213435E-921.4210538
GGCACTA957.1213435E-921.42105312
TCTTACA1059.786163E-1021.1428592
GTTTAAA450.003822925320.5555551
GTGTACG450.003822925320.5555551
CTAGGAC450.003822925320.5555553
AGTCGGT450.003822925320.55555511
GTACGCC450.003822925320.5555553
ACTATTC1001.2854798E-820.3515
CTATTCA1001.2854798E-820.3516
CTAGACT555.137686E-420.1818184
AGACGAT656.892481E-519.9230777
AAGACGG1403.6379788E-1219.8214285
GGTATCA3100.019.6935481
TACACAT851.2425226E-619.58823437
Produced by FastQC (version 0.11.5)