FastQCFastQC Report
Fri 10 Feb 2017
ERR1631600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212581
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.7206664753670365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13130.6176469204679628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11000.5174498191277678No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.2563728649314849No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3090.14535635828225477No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3000.14112267794393665No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2940.13830022438505793No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT2310.10866446201683122No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT2240.10537159953147271No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG2220.10443078167851312No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC2130.10019710134019502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGTA200.001838563137.03
TAATGTG200.001838563137.014
TCAGATA451.3182612E-424.6666662
GACGGCC451.3182612E-424.66666611
CGTCGTA451.3182612E-424.66666610
ACCGTCG451.3182612E-424.6666668
TACCGTC451.3182612E-424.6666667
CGTTATT551.8932164E-523.5454542
ATACCGT551.8932164E-523.5454546
GGCAGTC1500.023.4333348
GCATTCG400.001926040723.12522
GACCCGC652.6658017E-622.7692314
CCATGAC502.6920743E-422.210
TGACCCG502.6920743E-422.213
AGTCGGT1500.022.211
CAGTCGG1550.021.48387110
AACGAAC705.0697126E-621.14285714
TACCCTC450.0038157120.5555555
GCCTTAT1351.8189894E-1220.55555526
ATTCGTA450.0038157120.55555524