##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631600.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	212581
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.020683880497316	34.0	33.0	34.0	31.0	34.0
2	33.15327804460417	34.0	33.0	34.0	31.0	34.0
3	33.21852846679619	34.0	33.0	34.0	31.0	34.0
4	36.46225203569463	37.0	37.0	37.0	35.0	37.0
5	36.44973915825027	37.0	37.0	37.0	35.0	37.0
6	36.507124343191535	37.0	37.0	37.0	35.0	37.0
7	36.520455732167974	37.0	37.0	37.0	35.0	37.0
8	36.52555966902028	37.0	37.0	37.0	35.0	37.0
9	38.35712975289419	39.0	39.0	39.0	37.0	39.0
10	38.325518273034746	39.0	39.0	39.0	37.0	39.0
11	38.382371895889094	39.0	39.0	39.0	37.0	39.0
12	38.351498958044225	39.0	39.0	39.0	37.0	39.0
13	38.3831998155997	39.0	39.0	39.0	37.0	39.0
14	39.89793537522168	41.0	40.0	41.0	38.0	41.0
15	39.87712918840348	41.0	40.0	41.0	38.0	41.0
16	39.8280702414609	41.0	40.0	41.0	38.0	41.0
17	39.832454452655696	41.0	40.0	41.0	38.0	41.0
18	39.820571923172814	41.0	40.0	41.0	38.0	41.0
19	39.83568145789135	41.0	40.0	41.0	38.0	41.0
20	39.79762067164987	41.0	40.0	41.0	38.0	41.0
21	39.77357807141748	41.0	40.0	41.0	38.0	41.0
22	39.701262107149745	41.0	40.0	41.0	37.0	41.0
23	39.63380546709254	41.0	40.0	41.0	37.0	41.0
24	39.601587159717944	41.0	40.0	41.0	37.0	41.0
25	39.60210460953707	41.0	40.0	41.0	37.0	41.0
26	39.47722044773521	41.0	40.0	41.0	36.0	41.0
27	39.41664589027241	41.0	39.0	41.0	36.0	41.0
28	39.33723615939336	41.0	39.0	41.0	35.0	41.0
29	39.26954431487292	41.0	39.0	41.0	35.0	41.0
30	39.17772519651333	41.0	39.0	41.0	35.0	41.0
31	39.09962320244989	41.0	39.0	41.0	35.0	41.0
32	39.00134536952973	41.0	39.0	41.0	35.0	41.0
33	38.88463221078083	41.0	38.0	41.0	35.0	41.0
34	38.8133840747762	41.0	38.0	41.0	35.0	41.0
35	38.6909789680169	40.0	38.0	41.0	35.0	41.0
36	38.595655303155034	40.0	38.0	41.0	35.0	41.0
37	38.49333665755641	40.0	38.0	41.0	35.0	41.0
38	38.38911285580555	40.0	38.0	41.0	35.0	41.0
39	38.26621381967344	40.0	38.0	41.0	35.0	41.0
40	38.124423161053905	40.0	37.0	41.0	35.0	41.0
41	37.980849652603005	40.0	37.0	41.0	34.0	41.0
42	37.799229470178425	40.0	37.0	41.0	34.0	41.0
43	36.974386233953176	40.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	1.0
18	3.0
19	7.0
20	10.0
21	28.0
22	69.0
23	101.0
24	146.0
25	253.0
26	385.0
27	500.0
28	769.0
29	1105.0
30	1464.0
31	1878.0
32	2247.0
33	2914.0
34	4281.0
35	6020.0
36	9424.0
37	20195.0
38	39509.0
39	121270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60511522666654	17.31434135694159	12.43384874471378	25.646694671678087
2	19.534671489926193	20.182894990615342	33.21651511659085	27.065918402867613
3	20.38799328256053	22.430508841335772	28.685536336737528	28.49596153936617
4	14.208231215395543	15.693312196292236	33.01612091391046	37.082335674401754
5	15.70836528193959	35.76001618206707	32.025439714744024	16.50617882124931
6	34.15168806243267	33.4202021817566	16.524054360455544	15.904055395355183
7	30.319266538401834	30.02526095935196	20.18759907988014	19.467873422366065
8	30.214835756723318	30.30562467953392	19.18515765755171	20.294381906191052
9	28.19442941749263	13.689840578414817	19.09860241507943	39.01712758901313
10	19.233609776979126	25.479229093851284	29.42501916916375	25.862141960005836
11	36.98213857306156	21.84014563860364	19.533730672073233	21.643985116261565
12	23.46634929744427	25.897422629491818	26.767679143479427	23.868548929584488
13	31.490584765336504	18.13708656935474	24.697409458041875	25.67491920726688
14	23.760825285420616	20.177250083497583	24.080232946500395	31.98169168458141
15	27.367450524741155	26.15473631227626	21.39702042985968	25.0807927331229
16	26.099228058951645	24.559579642583298	23.998381793292907	25.342810505172146
17	24.433980459213196	26.13027504809931	24.212417854841213	25.223326637846277
18	25.11136931334409	23.01851999943551	26.288802856323002	25.581307830897398
19	25.007879349518536	24.923676151678652	25.907301216947893	24.161143281854915
20	25.526269986499262	23.050037397509655	26.330669250779703	25.09302336521138
21	27.51609974550877	23.50821569190097	25.339988051613265	23.63569651097699
22	26.773324050597186	23.19727539149783	24.925087378458095	25.10431317944689
23	25.048804926122276	23.670506771536495	25.511216900851913	25.769471401489312
24	24.313555774034366	24.77408611305808	25.822157201255052	25.0902009116525
25	24.82536068604438	23.674740451874815	25.547908797117337	25.95199006496347
26	25.230382771743475	24.70822886335091	25.62364463428058	24.437743730625034
27	25.52956284898462	23.72977829627295	25.149002027462476	25.59165682727996
28	24.223707669076727	24.166788188972674	26.48496337866507	25.124540763285523
29	23.964041941659886	24.3732977076973	26.838240482451397	24.82441986819142
30	23.288534723234907	25.36350849793726	27.080501079588487	24.26745569923935
31	24.741157488204497	24.863463809089243	25.40584530132044	24.989533401385824
32	23.838442758289784	24.249109751106637	26.309500849088113	25.602946641515466
33	23.55760862918135	23.814451903039313	26.90880182142336	25.71913764635598
34	24.941551690884886	24.128214657001333	25.727134598106133	25.203099054007648
35	24.587804178172085	24.31496700081381	26.448741891326126	24.648486929687976
36	23.04062921898006	24.943433326590807	26.351837652471293	25.66409980195784
37	24.04401145916145	23.97862461838076	26.80107817725949	25.1762857451983
38	23.233026469910293	23.01710877265607	27.90465751878108	25.845207238652563
39	23.900066327658635	22.937139255154506	27.769179747954897	25.39361466923196
40	23.35251033723616	23.188337621894714	27.847267629750544	25.611884411118584
41	21.983149952253495	23.670036362610016	28.827129423607943	25.519684261528546
42	22.762147134504023	23.605119930755805	28.848297825299536	24.784435109440636
43	21.610115673555022	23.102252788348913	29.047280801200483	26.240350736895586
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	30.0
2	35.0
3	117.5
4	200.0
5	200.0
6	287.5
7	375.0
8	362.5
9	350.0
10	527.5
11	705.0
12	705.0
13	1170.0
14	1635.0
15	2411.5
16	3188.0
17	2764.5
18	2341.0
19	2341.0
20	2538.5
21	2736.0
22	2028.0
23	1320.0
24	1382.5
25	1445.0
26	1445.0
27	1620.5
28	1796.0
29	2162.0
30	2528.0
31	2984.5
32	3441.0
33	3441.0
34	4113.0
35	4785.0
36	5737.5
37	6690.0
38	7665.5
39	8641.0
40	8641.0
41	9566.5
42	10492.0
43	11310.0
44	12128.0
45	13125.0
46	14122.0
47	14122.0
48	15457.5
49	16793.0
50	16723.0
51	16653.0
52	16181.0
53	15709.0
54	15709.0
55	15217.5
56	14726.0
57	13976.0
58	13226.0
59	13021.5
60	12817.0
61	12817.0
62	12000.5
63	11184.0
64	10413.5
65	9643.0
66	8520.0
67	7397.0
68	7397.0
69	6395.0
70	5393.0
71	4687.0
72	3981.0
73	3047.5
74	2114.0
75	2114.0
76	1717.5
77	1321.0
78	1099.0
79	877.0
80	767.0
81	657.0
82	657.0
83	523.0
84	389.0
85	357.5
86	326.0
87	296.5
88	267.0
89	267.0
90	190.5
91	114.0
92	63.0
93	12.0
94	8.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	212581.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.40782984000165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.5076769640571	49.662179990064196
2	7.165216766180841	8.22679089759273
3	1.9808373210509358	3.411467156028505
4	1.0069112404806244	2.312183566299866
5	0.606170882032851	1.739947742485281
6	0.4600888218658345	1.584762047817639
7	0.2959807934192247	1.1894130517163668
8	0.2472766137498523	1.1356491012450665
9	0.20456530622125962	1.0569285261646113
>10	1.294895325657723	15.03217044750746
>50	0.15491066631150313	6.082072160269008
>100	0.07149723060530914	6.006822978044291
>500	9.930170917404049E-4	0.3106879114581132
>1k	0.0029790512752212142	2.248924423306893
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1532	0.7206664753670365	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1313	0.6176469204679628	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1100	0.5174498191277678	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	545	0.2563728649314849	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	309	0.14535635828225477	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	300	0.14112267794393665	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	294	0.13830022438505793	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	231	0.10866446201683122	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	224	0.10537159953147271	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	222	0.10443078167851312	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	213	0.10019710134019502	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	9.408178529595778E-4	0.0
13	0.0	0.0	0.0	0.0014112267794393666	0.0
14	0.0	0.0	0.0	0.0018816357059191556	0.0
15	0.0	0.0	0.0	0.003763271411838311	0.0
16	0.0	0.0	0.0	0.007056133897196833	0.0
17	0.0	0.0	0.0	0.009878587456075566	0.0
18	0.0	0.0	0.0	0.010819405309035144	0.0
19	0.0	0.0	0.0	0.01223063208847451	0.0
20	0.0	0.0	0.0	0.013171449941434089	0.0
21	0.0	0.0	0.0	0.015993903500312823	0.0
22	0.0	0.0	0.0	0.022109219544550078	0.0
23	0.0	0.0	0.0	0.027283717735827755	0.0
24	0.0	0.0	0.0	0.039043940897822475	0.0
25	0.0	0.0	0.0	0.04610007479501931	0.0
26	0.0	0.0	0.0	0.057860297957014035	0.0
27	0.0	0.0	0.0	0.09831546563427587	0.0
28	0.0	0.0	0.0	0.22250342222494013	0.0
29	0.0	0.0	0.0	0.38714654649286623	0.0
30	0.0	0.0	0.0	0.6270550989975586	0.0
31	0.0	0.0	0.0	1.1200436539483773	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATAGTA	20	0.0018385631	37.0	3
TAATGTG	20	0.0018385631	37.0	14
TCAGATA	45	1.3182612E-4	24.666666	2
GACGGCC	45	1.3182612E-4	24.666666	11
CGTCGTA	45	1.3182612E-4	24.666666	10
ACCGTCG	45	1.3182612E-4	24.666666	8
TACCGTC	45	1.3182612E-4	24.666666	7
CGTTATT	55	1.8932164E-5	23.545454	2
ATACCGT	55	1.8932164E-5	23.545454	6
GGCAGTC	150	0.0	23.433334	8
GCATTCG	40	0.0019260407	23.125	22
GACCCGC	65	2.6658017E-6	22.76923	14
CCATGAC	50	2.6920743E-4	22.2	10
TGACCCG	50	2.6920743E-4	22.2	13
AGTCGGT	150	0.0	22.2	11
CAGTCGG	155	0.0	21.483871	10
AACGAAC	70	5.0697126E-6	21.142857	14
TACCCTC	45	0.00381571	20.555555	5
GCCTTAT	135	1.8189894E-12	20.555555	26
ATTCGTA	45	0.00381571	20.555555	24
>>END_MODULE