FastQCFastQC Report
Fri 10 Feb 2017
ERR1631599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258299
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.13279184201255134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2810.10878865191115722No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2740.10607861431906433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2740.10607861431906433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGAG250.00548855329.63
TCTTTAG250.00548855329.61
ATACACA2300.025.7391337
GGTATCA2600.024.1923081
TAGATAA603.711906E-521.5833324
AAGGGGT450.003817901520.5555556
CTTTAGA450.003817901520.5555552
GGTTCCG555.1282684E-420.1818188
ATACCGT759.220239E-619.7333326
TACACAT759.220239E-619.73333237
TACCGTC759.220239E-619.7333327
GCGATGC1052.239176E-819.3809532
TCGCACG609.210409E-418.522
GATCGCA609.210409E-418.520
TGACAGT500.007020481418.56
GGAGTAT609.210409E-418.518
GTTCCGG609.210409E-418.59
CGTCGTA801.6087757E-518.510
GGGTTCC609.210409E-418.57
ACCGTCG801.6087757E-518.58
Produced by FastQC (version 0.11.5)